Hi Alexandre,
If you are interested in model-based bioregionalisation there are several attractive options available in R. With colleagues, I have been trying to develop and implement model-based bioregionalisation to maintain species information and uncertainty throughout the modelling process. Here are a few papers which might be of interest to you. Model-based bioregionalisation perspective piece: https://academic.oup.com/bioscience/article/70/1/48/5670754?login=true Comparison of model-based methods: https://besjournals.onlinelibrary.wiley.com/doi/abs/10.1111/2041-210X.13447 Vegetation classification (bioregionalisation) using Regions of Common Profiles. https://onlinelibrary.wiley.com/doi/abs/10.1111/jbi.13088 [https://onlinelibrary.wiley.com/cms/asset/2bdffdcb-f397-4884-a522-117552992254/jbi.2017.44.issue-12.cover.gif]<https://onlinelibrary.wiley.com/doi/abs/10.1111/jbi.13088> Simultaneous vegetation classification and mapping at large spatial scales<https://onlinelibrary.wiley.com/doi/abs/10.1111/jbi.13088> Aim Multivariate mixture models offer the ability to streamline the typically multi�\stage process of vegetation classification and mapping into a single, simultaneous analytical step. Our aim is to ... onlinelibrary.wiley.com [https://besjournals.onlinelibrary.wiley.com/cms/asset/660a90cf-3de4-44bc-aac9-6b796528810d/mee3.v11.10.cover.gif]<https://besjournals.onlinelibrary.wiley.com/doi/abs/10.1111/2041-210X.13447> Determining marine bioregions: A comparison of quantitative approaches - Hill - 2020 - Methods in Ecology and Evolution - Wiley Online Library<https://besjournals.onlinelibrary.wiley.com/doi/abs/10.1111/2041-210X.13447> Areas that contain ecologically distinct biological content, called bioregions, are a central component to spatial and ecosystem�\based management. We review and describe a variety of commonly used... besjournals.onlinelibrary.wiley.com There is code available for the second paper here: https://github.com/Hillna-IMAS/Bioregion_Methods/tree/v1.1 The RCP approach can be implemented using the RCPmod package (on cran) or the updated ecomix package https://github.com/skiptoniam/ecomix which is still in development - but should be on cran soon. ecomix has all the functionality of RCPmod, but with some additional functions to make interpretation of these models a little easier. Anyway, I hope your bioregionalisation work goes well. I'd be happy to help with ecomix related questions if you decide to apply such methods. Cheers, Skip. Dear R-Sig Colleagues, I am performing a bioregionalization of the Cerrado woody flora in central Brazil. I am following the steps Produce a dissimilarity matrix from species x site matrix Perform a NMDS on the dissimilarity matrix Interpolate each NMDS axis with Kriging Partition the stacked interpolated rasters with K-means I am willing to change the k-means by the ISO DATA method implemented in ArcGis, which takes an initial number of groups (the same used for the k-means classification). The ISODATA algorithm has some further refinements by splitting and merging of clusters. Clusters are merged if either the number of members (pixel) in a cluster is less than a certain threshold or if the centers of two clusters are closer than a certain threshold. Clusters are split into two different clusters if the cluster standard deviation exceeds a predefined value and the number of members (pixels) is twice the threshold for the minimum number of members. It implements a set of rule-of-thumb procedures that have been incorporated into an iterative classification algorithm. Many of the steps used in the algorithm are based on the experience obtained through experimentation. The ISODATA algorithm is a modification of the k-means clustering algorithm and is supposed to overcome the disadvantages of k-means. The classification produced preserved the main patterns found in kmeans while reducing the number of groups. However, I have not found anything in the literature of bioregionalization using ISO DATA, only kmeans. Do you know of any reasons I should not use ISO DATA for this aim? Thank you very much in advance for any inputs, Best, Alexandre -- Dr. Alexandre F. Souza Professor Associado Chefe do Departamento de Ecologia Universidade Federal do Rio Grande do Norte CB, Departamento de Ecologia Campus Universit��rio - Lagoa Nova 59072-970 - Natal, RN - Brasil lattes: lattes.cnpq.br/7844758818522706<http://lattes.cnpq.br/7844758818522706> http://www.esferacientifica.com.br<http://www.esferacientifica.com.br> https://www.youtube.com/user/alexfadigas<https://www.youtube.com/user/alexfadigas> http://www.docente.ufrn.br/alexsouza<http://www.docente.ufrn.br/alexsouza> orcid.org/0000-0001-7468-3631<http://orcid.org/0000-0001-7468-3631> <http://www.docente.ufrn.br/alexsouza<http://www.docente.ufrn.br/alexsouza>> Dr. Skipton Woolley IMAS, University of Tasmania. Ph: 0419 990 927 University of Tasmania Electronic Communications Policy (December, 2014). This email is confidential, and is for the intended recipient only. 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