Hi Martin, A polynomial baseline correction in the direct dimension is essential. You'll see this in the baseplane noise if you drop the contour levels all the way down - certain horizontal rows will be slightly below and other above the average level of zero. Note that if you perform any extraction in the spectra to discard areas of pure white noise, you should do this at the very end after the polynomial baseline correction. The reason is because the baseline correction will operate on and significantly benefit from the data you throw away. I would also highly recommend using peak heights rather than volumes, as molecules exhibit their most interesting dynamics for peaks most often in the random coil region. These interesting spins often have peak overlap, and volume integration is much less accurate in such cases. I hope some of this helps.
Regards, Edward On 3 July 2012 11:36, Martin Ballaschk <[email protected]> wrote: > Dear relax-users, dear Edward, > > is a baseline correction recommended when processing spectra for relaxation > analysis? > > To my understanding, with baseline correction, peak volumes will be more > accurate, which is important for quantitative analysis. But won't weak > signals be distorted and hence bias introduced? > > So far, I've always performed a baseline correction. > I'd be interested in your opinion on this. > > Cheers > Martin > _______________________________________________ > relax (http://www.nmr-relax.com) > > This is the relax-users mailing list > [email protected] > > To unsubscribe from this list, get a password > reminder, or change your subscription options, > visit the list information page at > https://mail.gna.org/listinfo/relax-users _______________________________________________ relax (http://www.nmr-relax.com) This is the relax-users mailing list [email protected] To unsubscribe from this list, get a password reminder, or change your subscription options, visit the list information page at https://mail.gna.org/listinfo/relax-users
