Hi Troels, The -fn REV part is needed for certain acquisition modes. I copied the script contents for one such spectrum. This option can be removed for most 2D experiments though. Looking closely at the script I have noticed that there are a few things wrong with it or at least not ideal for a relaxation analysis. For example the extract function should be performed at the very end. In this case it does not matter as a polynomial baseline correction is not performed in the indirect dimension but, if it were to be added, performing the data trimming after a polynomial correction results in a better baseline. I must have copied the wrong script. I'll look into this and change the script contents to one of the scripts I use for processing spectra for standard R1, R2 or NOE spectra from back in my PhD days.
Cheers, Edward On 25 August 2013 18:45, Troels Emtekær Linnet <tlin...@gmail.com> wrote: > Hi. > > I was looking the in the manual, at page: 52 > 5.2.2 Spectral processing > > There is an example of processing script for NMRPipe. > It mentions the line: > | nmrPipe -fn REV \ > > which is for reversing the spectrum, since the CPMG element in a pulse > sequence will make the magnetization end up negative. > > According to the manual: > http://spin.niddk.nih.gov/NMRPipe/ref/nmrpipe/rev.html > > The recommendation is to do a negative Fourier Transform > > | nmrPipe -fn FT -neg \ > > Is there a special reason for | nmrPipe -fn REV \ > > Best > Troels Emtekær Linnet > > _______________________________________________ > relax (http://www.nmr-relax.com) > > This is the relax-users mailing list > relax-users@gna.org > > To unsubscribe from this list, get a password > reminder, or change your subscription options, > visit the list information page at > https://mail.gna.org/listinfo/relax-users _______________________________________________ relax (http://www.nmr-relax.com) This is the relax-users mailing list relax-users@gna.org To unsubscribe from this list, get a password reminder, or change your subscription options, visit the list information page at https://mail.gna.org/listinfo/relax-users