Hi Troels,

The -fn REV part is needed for certain acquisition modes.  I copied
the script contents for one such spectrum.  This option can be removed
for most 2D experiments though.  Looking closely at the script I have
noticed that there are a few things wrong with it or at least not
ideal for a relaxation analysis.  For example the extract function
should be performed at the very end.  In this case it does not matter
as a polynomial baseline correction is not performed in the indirect
dimension but, if it were to be added, performing the data trimming
after a polynomial correction results in a better baseline.  I must
have copied the wrong script.  I'll look into this and change the
script contents to one of the scripts I use for processing spectra for
standard R1, R2 or NOE spectra from back in my PhD days.

Cheers,

Edward



On 25 August 2013 18:45, Troels Emtekær Linnet <tlin...@gmail.com> wrote:
> Hi.
>
> I was looking the in the manual, at page: 52
> 5.2.2 Spectral processing
>
> There is an example of processing script for NMRPipe.
> It mentions the line:
> | nmrPipe -fn REV \
>
> which is for reversing the spectrum, since the CPMG element in a pulse
> sequence will make the magnetization end up negative.
>
> According to the manual:
> http://spin.niddk.nih.gov/NMRPipe/ref/nmrpipe/rev.html
>
> The recommendation is to do a negative Fourier Transform
>
> | nmrPipe -fn FT -neg \
>
> Is there a special reason for | nmrPipe -fn REV \
>
> Best
> Troels Emtekær Linnet
>
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