On 1 November 2016 at 22:12, Mahdi, Sam <sam.mahdi....@my.csun.edu> wrote: > Hello everyone, > > I had a quick question regarding running the single model free run (m4). > For some proteins we only have data at only one field strength, so I know I > can't use the d'Auvergene protocol (needs a minimum of 4), so I was > thinking I could run the all models free model. The problem is, I know > models m6-m9 are models for another field strength, but since I don't have > data at another field strength, could I just remove them? Or will relax > just not run m6-9 since I've only loaded one field strength?
Hi Sam, The protocol I designed for iteratively optimising and fine tuning the coupled diffusion tensor + model-free optimisation and model selection problem will only work with 2 or more fields. If you would like to understand this problem, please read: d'Auvergne E. J., Gooley P. R. (2007). Set theory formulation of the model-free problem and the diffusion seeded model-free paradigm. Mol. Biosyst., 3(7), 483-494. (http://dx.doi.org/10.1039/b702202f) From this, hopefully you will understand why the NMR field will not take any results from a single field strength analysis seriously. Note that this has been the case since the mid 1990's. Regards, Edward _______________________________________________ relax (http://www.nmr-relax.com) This is the relax-users mailing list relax-users@gna.org To unsubscribe from this list, get a password reminder, or change your subscription options, visit the list information page at https://mail.gna.org/listinfo/relax-users