Another question is POS is the 1-based leftmost POSition in the case of both 
reverse and forward strand or is the 1-based RIGHT most POSition in case of 
reverse strand?

Look forward to your reply,




On Sunday, June 1, 2014 8:26 PM, carol white <wht_...@yahoo.com> wrote:
 


Hi,
Just wanted to check that if a sam file is viewed by samtools -X, if there is 
no value in the 2nd column, the strand direction is forward (+) since u is 
unmapped and r is reverse (-)?

Cheers,

Carol
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