Hi Thiagp

The format, including the last 3 columns is described here: 
http://samtools.sourceforge.net/pileup.shtml

Best,
Devon

____________________________________________
Devon Ryan, Ph.D.
Email: dpr...@dpryan.com
Tel: +49 (0)178 298-6067
Molecular and Cellular Cognition Lab
German Centre for Neurodegenerative Diseases (DZNE)
Ludwig-Erhard-Allee 2
53175 Bonn, Germany

On Aug 4, 2014, at 9:48 PM, Thiago M. Venancio wrote:

> Hi all,
> 
> I ran the samtools mpileup on a set of mapped reads (in bam format). The 
> output gave me something like that:
> 
> supercontig_0   30      T       2       ^g.^g,  BH
> supercontig_0   31      G       2       .,      AG
> supercontig_0   32      C       2       .,      BH
> supercontig_0   1689    T       4       .,^g.^g,        GBA5
> supercontig_0   1690    A       4       .,.,    GBAD
> supercontig_0   1691    A       4       .,.,    BAAF
> supercontig_0   1692    C       4       .$,$.,  EA?C
> 
> ​I inspected all the documentation I could find over the past few hours and 
> was unable to find ​a complete explanation for this output. I understand the 
> first three columns, but not the other three.
> 
> Can anyone point me the appropriate documentation ?
> 
> Thanks in advance.
> Thiago
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