Hi all, We have an amplicon pipeline that uses mpileup output. We recently updated samtools from 0.1.19 to 1.1. When performing some validation on the pipeline, we found that there were a significant number of variants missing from the caller(Varscan 2.3.7) output. The only difference was the output from mpileup.
In the version 1.1, the coverage is greatly reduced and the base quality string is the same upto approx the middle (base quality string position ~1138); after that everything appears to be garbled. I’ve included outputs for one position, but it is pretty much the same for most positions. (see outputs below) I used the same command is for both versions: samtools mpileup -B -f /local/scratch/REFERENCE_DATA/GATK_Bundle/b37/human_g1k_v37_decoy.fasta -r 13:32911471-32911471 Sample.bam I’ve searched for an answer to the differences, but couldn’t find an explanation of why the outputs of v1.1 would be reduced and changed compared to 0.1.19. If there is an option to get similar output to v0.1.19, that would be great. Or, if it’s a bug it would need to be fixed. Any help or a point in the right direction would be appreciated. Thanks, Paul Paul Lott UC Davis Genome Center University of California, Davis Using samtools 0.1.19, we get the following output: [mpileup] 1 samples in 1 input files <mpileup> Set max per-file depth to 8000 13 32911471 G 3656 ,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,a,,..AA.....AAAAA.A.A.A.A.AAAA.AAA.A.AAA...AAAAAA..A..AAA.AA.AAA.A.A.A.AA..A....A.A.........A..A.A...A...AAA...AAA.....AA.....A.A.AA...A.A.A..AAAAAA...A.AA..A...AA..A....A.A.A..AAAA..AAA.A....A...A.A..AA..A.A..AA..A....A.AAA.....A..A....AAA..A.AAAAA..........A..AAA..A...A..AAAAA..A......A....A..AA...A.AAA.A....A..AAAAA.A.AA.AA....AA.A.A..A.AA..AA..A....AA.AAAAAA.....A.AAA.AA.AA.A..AAAAA...A.......AA..A.AAA...A.....A.....AAAA..A...AA.A...A..AA..A..AA..A.A..A..AA..AAA.AAA.....AAAAAAA.A.A.A...AA..A......AAAA.AAA.A.A..AAA.......AAA..A.AA..AAA..A...A.A...AA.AA...AA..A.A...A...A.AA.A..AA.AAAA.AA.AAAA...AA..AAAA.A...AA.....AA...A....A.A..A.A.A.AAA.AA.A........AAAA.A.AA.AA..AA..AAA..A..A.A.......A..AA.A...A..AAAA..AA.A..A.A...A.A...A......AA..A.A...A......AAA.A.A..A....AA..AAA.AAA.A.A...AA..AA..A.AA..AA.AAA..AA...AAAAAA.A.A......A....A.A.AA..A...A...A.A..A.A..AA...A....A.....AA.A..AA....A.A..AA.A.AA......A.AA..A.AAAAAAAA....AA..A.A..AAAAA.A.A...A.A..A....A..AA..AAAAA..AAAAA.A.A...A..A..A.A....AA.AA..A..A...A..A.AAAA.A.......A.A...A..A....AAAAA.AAAA....A.....AA....A.AAAA...A.A...AA....AAAA..AA....AAAA.A.....AAA.A.A.AA.A.AA....A.AA.AA...A.A............A...AA..AA..AA..AA...A..A..A..A.A.....AA...A..A.A..AAA.A.A.A..A....AA.A..AA..A....A...A.A...AAAA.A.A.AA.AAAAAA...AA.....AAA.AA.,.A.,,,,,,,,,,,,,,,,,,,,,aa,,,,a,,,aa,aa,aa,,,,,,,,a,a,,a,,,aaa,a,,,,,,a,,a,,aaa,a,a,a,aaa,aa,,aaa,,,aaaa,,a,,aaa,aaaa,a,a,a,aa,,a,aa,,,,,aa,a,,a,,,aaa,,aa,,,,,aa,,,aa,a,a,a,a,aa,,,aa,,a,,,,,a,,,a,,aaa,aaa,,a,,a,,a,a,a,aa,,,,a,aa,,,,a,,,,aaa,aaa,,,,,,,,a,,aa,,a,,,aaaaa,,a,,,,,,a,,aa,,,,aa,,,,,,aaa,a,a,aaa,,,,aa,a,,a,aa,,a,,a,,,aa,,,,aaaa,aa,aa,a,,aaaaa,,,,,aa,a,aaa,,a,,a,,,,aaaa,,,,aa,,,,a,,aa,a,,aa,,a,aa,,aa,,aaa,aaa,,,,aaaa,a,,a,,,aa,,,,,,aaa,aaa,,,aa,,,,,,aa,a,a,aa,,aaa,,a,,aa,,,aa,aa,,,a,,,aa,a,,,,aaa,aaaaa,a,aaa,,,aa,,aaa,a,,,a,,,,a,,a,,,,a,,aa,aa,aa,a,,,,,,a,aaaa,a,aa,a,aa,,,,aaa,a,aa,a,,a,,,a,a,,,,a,,,,,,a,,,,,,a,,,aa,a,,a,,,aa,,aaaa,a,a,,a,a,,a,aa,,aa,a,,aa,aaaa,,,,,,,,,a,,aa,,,a,a,,aa,a,,,,,,a,,,,,aa,,a,a,,aa,aa,,,,,a,,a,aaa,,a,a,a,,aaaa,a,,,a,,,a,,,a,,,,aaa,aaaa,a,a,aa,a,,,aa,aa,,,a,,aaaa,a,,,,,,a,,,,,a,,,aa,,,,,,aa,,,,aaaaa,a,a,,,,,aa,aa,,,aa,a,aa,a,a,aa,a,aa,,a,aaa,,,,,,,a,,aa,,aa,,aa,,a,,,a,a,a,,,,aa,,a,,a,a,,aaa,aaa,a,,aaa,,aa,,a,,,,a,aa,aaaa,a,aaaaaa,a,,,,aaa,a,,,aa,a,a,a,,a,a,,,a,,,,,,a,,,,,aaa,a,,,,a,,,,a,,,,aa,,,,,a,t,a,,,,a,,aaaa,,aaa,a,,,,a,,a,a,,,a,a,a,a,aa,,aa,aa,aaaa,aa,,,aaa,aaa,,,a,aa,,,aaaa,,a,,a,,a,,,,a,a,a,a,,,,,a,,aaa,,,,a,,a,aa,,,a,,,,,a,,,,,a,aa,aaaaa,a,,a,,,,,,,,,,,,,,aa,a,a,a,, GGGGGGGGGGGEGGGGGGGGGGGGGGGGGGGGGGG<GGGGGGG<GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGFGGGGGGGGGGGGGGFGFGFGGGGGGGGGGGGGGGGGGGGGGGFGGGGGGGGGGGGGGGGGGGGGGGGGGGGFGGGFGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGDGGGGGGGGGGGGGGGGGGGGGGGGGGFGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGFGGGGGGGGGGGGGGGGGGGGGGGGGFGGGGGGGGGGGGGGGGGGGFGGGGGGGGGGGGEGGGGGEGGGGGFEGGGFGGGGFGGGFGGGG<GGGGGGGGGGGGEGGFGGGGGGFGGGGGGGGFGGGGGGGGGGGFGGGGGCGGGGGGGGGGGGFGGFFGGGGFGGGGGFGGGGGGGGGGGGGGGGGGGGFGGGGGGGGGGGGGGGGGFGGGGGGGGGFGGGGGGGGGGGGGGGGGGGGGGFGGGGGGEGGGGGGGGGGGGGGGGGFGGGGGGGGGGGGGGGGGGFGGGGGGGGGGGGGGGFGGGGGGGGFGGGGGGGGGGGGGGGGGGGGGGEGGGGGGGGFGGGGGGGGGGGGGGGGGGGEGFGGGGGGGFGGGGGGGGGGGGGGGGGFGGGGDGGGGGGGGGGGG@GGGGGGGGGGGGGGGGGFFGGGGGGEGGGGGGGGGGGGGGCFGGCGGGGGFGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGFGGGGGGGFFGGGGFGGFGGEGGGGGGGGGGAEFFGFGGC<FGGGDDGGEGGGGGGGDFG8GGGGGGGGGGGGGFGGGGGFGGGGGGFFEGGGEGG@F8FGGGGCFGGFGGGFGGGFFFG8GEFGGGGGCGGGGGFGFGGGGGFGGGCGGGGGG@@FDGGGEGECGGGC8GGGCG<FFFGGGGAGGFGGGGFGGGGGFCGGGGGGGGGFGDGGGGEGGFFFEGGGFC@GGGG8GGG8GDFGEFGGFGGFGGF8GGGGFGGGGAFGEGEFGCFFFCCGDGGGGEGFGFGG?GGGFFGC8FGDGGDFFC8GFGC<GEFFG<GC8GG@GFFDGGFF88G<F@FGGGGF@GF<GFFFGGFFD<E@G8GGGGGGEGFGGGGGGGGCGGGGGGGGGGGGGGGGGGAGGFGGGDGGGGGGGGGGGGGGGGGGGEGGAGGGGGGGGGGFGGG9FGGGGG@CG@GGEGGGGGGGGGGGECGGGDGGGGGGGGGGGGFGGGGGFFGGGGBGGGGGGGGGGGGFGGGGGG<GGGGGGGG?GGGGGEGFGGGDG9GGFGGGGGGGGDGGFGGGGGGGGGGGCGGGGGGGGGFGDGG@GGGGGGFGGCGGEGGFFDGGG<GGGGGGGGCGGF<GGGFGGFGGGGGGGGGFGGFFGGGGFGGGGGGCGGEGGGGGGGGGGGGDEGGGGGGFCGGGGGGGGGGFGGGGGGFGGGGGGDGGFGGGGFF9FE9FGGGGFFFGGGGCFGGFGFGGGDGGGGFGGGGGGGGGGGGGGG5GGGGGGAGEGG8GGGGGGGGFGGCGGGGGGGGGGGGFGGFGGGEGGFGGGGGGGFGGGGGGGGGGDGGGGGGGGGGGGGGGGGEGGG<DGGGGGGGGGGGDGGGGGFGGFG9GGGGGGGAGGGGGGFFEGGGGGGGGGGGCGFGG<G9GGGGGGGGGCGGGGGGGGGGGGG9CFGGGGFGGGFGGFGGEGFGGGGFGFGGG<GGGGGGG9<FDGGGFGGFGGGGGGGGGGGGGEGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG<FGEGGGGFGDGGGGGAGAGGCG9FGGGGG<F<GGGFGGGGGFGGGGGAGGCGGGGGGGGGGG@GCGGGGDGGF9GGFGGGGGGFGGGGGCGGGFGGGGCGF<GGFGGGGGGGGEGDFGGGGGGGFGFGFGGGGGGGGGGGGGGGFAGGGGGGFGGGGGGCFGGGFGGGGGFGFE<GGGGGGGGG9FGGGGFGFFGGGGGG<FGFFGGCGGEGGGGGCGGFG@GGGFFGGGGGFGGGGGFGGGFGGGGFCGGGGGGGGGGGGGGGGFGGGDGGGFGGGGGGG@GGGFGGGGGFGGFGGFGFGFFGGGGGGGGGGGGEGGGGGGGG?GDGFGFGGCG9GGGGGGGFGGGC9GFGGFGGGGGGGGGGGAGGCGGGGGGCG9FGGGGGFFGGFG<GFGAGFFCGGG@GGGGFGCFGGGGGGFGGGGGGGGCGGGGGGGG9FGDEGGG9GGG9GGGG?GGFGFGGGGGGGGGGFGGGGGGGGGDAGFGGGGGGFFGGGFGGGGG?DGGCGGGGGGGGGGGGGGGDGG9FGEGGGGGGGFGGGGGGG<GGGGGGGGGDGAGGGFGGGFAGFGGGFGGGGFDGGGGGGGGGGGDGGFG9G=GGGGGGGGFGGGGGGGGGGGGGGGGFCF9FGGCFGCFGG8FFFCGCGCDG<FFGGFGFFGFGGGCCGGEFECFGGGGDGGGGGDCCGFGGGEFGFGGGFGGGFCGCGDD8GGGFGGF8FFGGFGGF=DGGGCFGFGGFGGG8GGGFGGGGGGGCGGFFGGFFDGG8GGG6FGDGFGEGCEGGGGFGDGGGG8GGG8GGEGGGGGGGFFGGGCDFGFFGGGGGFDGGGGGFFG8FGGFDGGGGGGEGGGG@8DGFGGGF8GGGGFGGGGDDFEGGGGGGGGGGGFGG8GDFGGGGGFEGFGGCGGGGGGGGFFG?GG=?G=GGGGGCGGGGFFGFGGGGFGG?GFGGFGGFGDF8FFGGGFFGGFGGDDFG?FGGGGGFGGGGE8G8GGGFGGDEFGGGFGGGFGGGGFFFDGGGGDEFGGGGGGFGFDFFF@GGGGGGGGFCGGGGGG8FFDGGDGF8GGCGGGGGFGDGGG8GGGCGGCD=GGGG8GGGGGFGGGCFCGEFGGG=GGGFGGGGFGGFGGGDGGGG=FG@DGDGG?FCFGG8GGGFGCF8GDFGF8B@GEF@GEGFGDGGGFGGGEFGFGFGCGFGGFGGFFFGGGGGGFG=CCFGGFGEGFGGFFGGGFFEGFGFGGDGG?GGGFGGDGDGFGGDGGGGGGGGGFFCGGGG=GGGFGFFFFGGGDGCGFGGGGGGF8GGGGEGGFGGDGGFGGF8GGFF?GGGFFGFGGGGGGGGGG=GFGGGGGFFGDGCGGFGG8GGGGDCFGGGGFGGGGGFGGG8G8CGGFGFGGFGDGFGGGGGG8G8G8GG8DGFGGFFFGDCGFGBG@GG8GGGFGGGGGGGGFFGFFFGGGFGGDGFG8FGFGGGG@G8DFGGEGGGFGGFGCFGGGGGGFG@GGGFDGGEDGGGEGEGFFF=GFGC?GGG8GGGGGCFDG=GGGGGGGGGGGGFGGGGGGGCGGGEF8FGFF=GFFGG8GGGFDGGFFDGGFFGGGFG>GGGFFFG8GGGFFGGFG8FG?GGGFG=GGFGGGGGGGDGGCGFFGD<DGFGGGFGG=GGGGGG8GGGGGGGGDGFG?=FGGG@GGFGFFGGC8GFGFGDGF8GFGBGE8FFFG8@?GGFEGG?F@GD@=8FFDG8G=GF=8F@FFDG8GGF8G=G88GE=G@C8@@GDFFGDGGGFGF?DCG@=G8?DGGGGDFDFG8G@?@GFDFGDEFGGDDCFGG6FFGG?FG=GD=GG8DFFCGFDG@@8=8B@F8CGG=GDGDGC@DGFG8EGG@CDFDF6G8GF Using Samtools 1.1, we get the following output: 13 32911471 G 2474 ,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,a,,..AA.....AAAAA.A.A.A.A.AAAA.AAA.A.AAA...AAAAAA..A..AAA.AA.AAA.A.A.A.AA..A....A.A.........A..A.A...A...AAA...AAA.....AA.....A.A.AA...A.A.A..AAAAAA...A.AA...A...AA..A...A.A.A..AAAA..AAA.A....A...A.A..AA..A.A..AA..A....A.AAA.....A..A....AAA..AAAAAA..........A..AAA..A...A..AAAAA..A......A....A..AA...A.AAA.A....A..AAAA.A.AA.AA....AA.A.A..A.AA..AA..A....AA.AAAAAA.....A.AAA.AA.AA.A..AAAAA...A.......AA..A.AAA...A.....A.....AAAA..A...AA.A...A..AA..A..AA..A.A..A..AA..AAA.AAA.....AAAAAAA.A.A.A...AA..A......AAAA.AAA.A.A..AAA.......AAA..A.AA..AAA..A...A.A...AA.AA...AA...AA...A...A.AA.A..AA.AAAA.AA.AAAA...AA..AAAA.A...AA.....AA...A....A.A..A.A.A.AAA.AA.A........AAAA.A.AA.AA..AA..AAA..A..A.A.......A..AA.A...A..AAAA..AA.A..A.A...A.A...A......AA..A.A...A......AAA.A.A..A....AA..AAA.AAA.A.A...AA..AA..A.AA..AA.AAA..AA...AAAAAA.A.A......A....A.A.AA..A...A...A.A..A.A..AA...A....A.....AA.A..AA....A.A..AA.A.AA......A.AA..A.AAAAAAAA....AA..A.A..AAAAA.A.A...A.A..A....A..AA..AAAAA..AAAAA.A.A...A..A..A.A....AA.AA..A..A...A..A.AAAA.A.......A.A...A..A....AAAAA.AAAA....A.....AA....A.AAAAA...A.A...AA....AAAA..AA....AAAA.A.....AAA.A.A.AA.A.AA....A.AA.AA...A.A...........A...AA..AA..AA..AA...A..A..A..A.A.....AA...A..A.A..AAA.A.A.A..A....AA.A..AA..A....A...A.A...AAAA.A.A.AA.AAAAAA...AA.....AAA.AA..A.,,,,,,,,,,,,,,,,,,,,aa,,aa,a,aaa,,,aaa,aa,,a,a GGGGGGGGGGGEGGGGGGGGGGGGGGGGGGGGGGG<GGGGGGG<GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGFGGGGGGGGGGGGGGFGFGFGGGGGGGGGGGGGGGGGGGGGGGFGGGGGGGGGGGGGGGGGGGGGGGGGGGGFGGGFGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGDGGGGGGGGGGGGGGGGGGGGGGGGGGFGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGFGGGGGGGGGGGGGGGGGGGGGGGGGFGGGGGGGGGGGGGGGGGGGFGGGGGGGGGGGGEGGGGGEGGGGGFEGGGFGGGGFGGGFGGGG<GGGGGGGGGGGGEGGFGGGGGGFGGGGGGGGFGGGGGGGGGGGFGGGGGCGGGGGGGGGGGGFGGFFGGGGFGGGGGFGGGGGGGGGGGGGGGGGGGGFGGGGGGGGGGGGGGGGGFGGGGGGGGGFGGGGGGGGGGGGGGGGGGGGGGFGGGGGGEGGGGGGGGGGGGGGGGGFGGGGGGGGGGGGGGGGGGFGGGGGGGGGGGGGGGFGGGGGGGGFGGGGGGGGGGGGGGGGGGGGGGEGGGGGGGGFGGGGGGGGGGGGGGGGGGGEGFGGGGGGGFGGGGGGGGGGGGGGGGGFGGGGDGGGGGGGGGGGG@GGGGGGGGGGGGGGGGGFFGGGGGGEGGGGGGGGGGGGGGCFGGCGGGGGFGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGFGGGGGGGFFGGGGFGGFGGEGGGGGGGGGGAEFFGFGGC<FGGGDDGGEGGGGGGGDFG8GGGGGGGGGGGGGFGGGGGFGGGGGGFFEGGGEGG@F8FGGGGCFGGFGGGFGGGFFFG8GEFGGGGGCGGGGGFGFGGGGGFGGGCGGGGGG@@FDGGGEGECGGGC8GGGCG<FFFGGGGAGGFGGGGFGGGGGFCGGGGGGGGGFGDGGGGEGGFFFEGGGFC@GGGG8GGG8GDFGEFGGFGGFGGF8GGGGFGGGGAFGEGEFGCFFFCCGDGGGGEGFGFGG?GGGFFGC8FGDGGDFFC8GFGC<GEFFG<GC8GG@GFFDGGFF88G<F@FGGGGF@GF<GFFFGGFFD<E@G8GmmmmmjiFmlimmmjjX^fmmmlmml^lmllemmldmlllcjjmmilmml^mmlmmm^mmmlEmmAmmmmmimmlllmmmWklRjmmdZmfmmhmjm\lmjmilmiiikmjGmmclmjmmmm^lmmjm^dlmGkmemmmmmmmmlcmillmmmil<GRmmllmml[mmmjjjFmmmDm_mlFlmm^GmmjDllejmGmmmmmmmRgmmmmmlf^jlkDlm^mml^RmlmmhlmklGllij^jbmGlmmm^fmmlbmmmlmllmm^mjlmmmlmlFllkmllmimfmmikikmmmmmllmemml`PefmmcmlimmmimGmmmklRmmmmllmmemGOlmllmmlli9k\^kmmmmFQlimllNbmmkmFmRjglmellmmmmlmmmmk^mmmc[m^mmjmfmkmj\lmmmlmmmkmmflmmmmmllljmmimmljklhmmlmmmlmmlilllfmmmmmGjmmmlimmmmmm^lljmmkjmlaim^mmimlmmmmimjjmRlmm]m_^mimmij]mmmGmmlFPmmmmmlfmmilCilklbm_cmmmlmmmmCmlmmlmmmjmmmmP_Qlfj^lmjmleRkil\mkm^mmlmllmiSl^mjhlm^Sgcmkl>fmjmlmGjmmimlmRmEmklilmlm^^imm?mlmmlfmmlllmRGmmmmml6FfkRmcidiilmmmlgmAkmilNlmmllm<l<mmfFmllmk>mll^mfmGCmmelmmmmlmm@mfjmlmbmGF\mmFmGmmmjlRmmllemmmemmlm_mi^mmkmmllllmmkjfbmjimlmmlmkmFmmll^mmmmkmmlmmFAjmmlmmhmll^mmikclldmimmllll\blmmmmmmmm^FmmmllmFQcmlmmm<lfllmkhlmhmmmimiRRFGemm^llmmmmjhlmmmmjlmklmmmm]hilmmm^mm^kl?ilGmljllgmlmllmmjmll@mmmlmm^mR]mGFmm]jlllFmmGllljmlmjiklmhmfmmjJmjmli]mmhm^mmmmmmmllllh^mFilklmlmmfmlmmjgmli^m^lmlhm_lGmfmkkFmmlfSjQlAmll_kmm@mlmmkmeFlmmRmmmdmmlmfmlmCmmmjljGm_lkfhmm^_mkm]mmcfellblbmlmiemmm^mlm^mmmRimGDflljjljmmbkmmlmmmmmejmmimlmmmmmmmiGmmmkjllXk^klml^mlclmllmmm^bmmljmmlljjmfllllmmmlblFdmmlmllll[mmmllmjfmGlj^l]lUmbeGmmRmmllcmmlmiRmGmGmmmmjFCF^kmbFGCFGG8FFFCGCGCDG<FFGDFGEF8GDD8FGF<=F8DFFGGGDG8 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