Hi,
I am using bcftools-1.3.1/htslib-1.3.1/tabix* to index the dbNSFP file to be
used by the program SnpSift, using the following commands and am getting an
error:
$ (head -n 1 dbNSFP3.2c_variant.chr1 ; catdbNSFP3.2c_variant.chr* | grep -v
"^#" ) > dbNSFP3.2c.txt
# Compress using block-gzip algorithm
bgzip dbNSFP3.2c.txt
# Create tabix index
tabix -s 1 -b 2 -e 2 dbNSFP3.2c.txt.gz
The first two steps work fine, but the tabix gives me anerror:
[E::hts_idx_push] unsorted positions
tbx_index_build failed: dbNSFP3.2c.txt.gz
In the dbNSFP file I have multiple lines with same chromosome and position, is
that what is giving this error? How do I fix this issue?
Thanks,Arti
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