Yes, samtools is open source, under the MIT/Expat License, see:

https://github.com/samtools/samtools/blob/develop/LICENSE

Peter

On Wed, Oct 12, 2016 at 6:19 PM, Apoorva Kulkarni
<akulka...@perthera.com> wrote:
> Hi,
> I wanted to use Variant Calling option of samtools.
> However I am confused on whether SAMTOOLS is an open source software as I am
> a commercial user.
> Eagerly waiting for reply,
> Thank you,
> Apoorva
>
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