On 9 Jan 2017, at 19:05, Madolyn Macdonald <mm...@udel.edu> wrote:
> I am trying to calculate the mapping statistics of a BAM file. I am using 
> SAMtools 1.3.1 and am able to get statistics on the BAM file using samtools 
> flagstat. However, when I try samtools stats, which I would like to use, I 
> get the following error:
> 
> [W::bam_hdr_read] EOF marker is absent. The input is probably truncated.
> [E::inflate_block] inflate failed: progress temporarily not possible, or in() 
> / out() returned an error
> [E::bgzf_read_block] inflate_block error -1
> [E::bgzf_read] bgzf_read_block error -1 after 0 of 4 bytes
> Failure while decoding file

Samtools flagstat and stats use the same code to read BAM files, so it would be 
surprising if one but not the other produced these messages when reading the 
same file.  Please show us the complete command lines you are using for both 
flagstat and stats.  After samtools stats has given those errors, does a 
subsequent samtools flagstat still work or does it produce similar messages?

    John

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