Hi,

many samtools commands expect the input to be either name-sorted or
coordinate-sorted, respectively.

Is it guaranteed that the output of these commands is still in the same
sort-order as the input?

For example, when invoking a command like:

samtools fixmate [*-rpc*] [*-O* *format*]

*in.nameSrt.bam out.bam*
...is out.bam guaranteed to still be name-sorted?

Same for rmdup etc.

I am doing a process along the lines of fixmate --> rmdup --> index -->
flagstat and I am wondering if I have to sort again before each
intermediate step.

Thanks,
Anton

--
Version used:
samtools 1.3.1
Using htslib 1.3.1
Copyright (C) 2016 Genome Research Ltd.
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