Dear Samtools Support,

When I submit the following command I got the following error messages:

bcftools +fixref GWA1MHCregionforHRC.vcf.gz  -Ov -o 
GWA1MHCregionforHRCfixref.vcf -- -d -f All_20151104.vcf.gz
[fai_load] build FASTA index.
[fai_build_core] different line length in sequence '(null)'.
Failed to load the fai index: All_20151104.vcf.gz

The All_20151104.vcf.gz and the All_20151104.vcf.gz.tbi files were downloaded 
based on the information from the fixref plugin webpage:
wget 
ftp://ftp.ncbi.nih.gov/snp/organisms/human_9606_b146_GRCh37p13/VCF/All_20151104.vcf.gz
wget 
ftp://ftp.ncbi.nih.gov/snp/organisms/human_9606_b146_GRCh37p13/VCF/All_20151104.vcf.gz.tbi


I re-built the All_20151104.vcf.gz.tbi file using the command: tabix -p vcf  
All_20151104.vcf.gz and re-submitted the above command and still got the same 
error messages. How should I proceed with this?

Thank you very much in advance!

Ying

Ying Jin, M.D., Ph.D.
Senior Instructor,
Human Medical Genetics and Genomics Program
Department of Pediatrics
University of Colorado School of Medicine
12800 E. 19th Ave., Rm. 3400, mail stop 8300
Aurora, CO 80045 USA

Tel. 303-724-3110
Fax 303-724-3100
Email ying....@ucdenver.edu<mailto:ying....@ucdenver.edu>




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