On Wed, Oct 31, 2018 at 10:02:12PM +0000, Yarza, Fauna wrote: > this error will be returned > Failed to open file ./samtools.7711.2619.tmp.0252.bam samtools sort: fail to > open "./samtools.7711.2619.tmp.0252.bam": Too many open files
You can reduce the number of files it attempts to merge from by increasing their size, which is governed by the samtools sort -m parameter. Try setting this to be something higher ("free -h" or "top" can give you an idea of how much you have on your system). Just be warned that this is the memory *per thread*, so if you specify multiple threads then it'll be much more than you think. James -- James Bonfield (j...@sanger.ac.uk) The Sanger Institute, Hinxton, Cambs, CB10 1SA -- The Wellcome Sanger Institute is operated by Genome Research Limited, a charity registered in England with number 1021457 and a company registered in England with number 2742969, whose registered office is 215 Euston Road, London, NW1 2BE. _______________________________________________ Samtools-help mailing list Samtools-help@lists.sourceforge.net https://lists.sourceforge.net/lists/listinfo/samtools-help