Dear all,
I am new for samtools that I am try to genrate index with  "samtools index -b 
bai_R1_trimmed20-3.bam” since I need the .bai format. But no matter -b is 
given, I always get .csi format index.

Alignment:
/local/prog/hisat2-2.1.0/hisat2 -x 
/mnt/scratch/bai010/ribo-seq/ribo_pipe/genome/TAIR -U bai_R1_trimmed20.fastq.gz 
-S bai_R1_trimmed20-3.sam -k 1 --min-intronlen 30 --max-intronlen 3000
sam to bam:
samtools sort -o bai_R1_trimmed20-3.bam bai_R1_trimmed20-3.sam

Anyone could help?
Bing



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