Dear all, I am new for samtools that I am try to genrate index with "samtools index -b bai_R1_trimmed20-3.bam” since I need the .bai format. But no matter -b is given, I always get .csi format index.
Alignment: /local/prog/hisat2-2.1.0/hisat2 -x /mnt/scratch/bai010/ribo-seq/ribo_pipe/genome/TAIR -U bai_R1_trimmed20.fastq.gz -S bai_R1_trimmed20-3.sam -k 1 --min-intronlen 30 --max-intronlen 3000 sam to bam: samtools sort -o bai_R1_trimmed20-3.bam bai_R1_trimmed20-3.sam Anyone could help? Bing _______________________________________________ Samtools-help mailing list Samtools-help@lists.sourceforge.net https://lists.sourceforge.net/lists/listinfo/samtools-help