On Tue, Oct 25, 2022 at 04:25:32PM +0200, Carla Mariner Llicer wrote: > I'm using Samtools version 1.3.1. I was analysing some illumina paired-end
Please use a modern version. 1.3 is 6 year old, with many bug fixes and improvements made since then. This particular issue was fixed in 1.13. However, also see wish-list item https://github.com/samtools/samtools/issues/1354 for potential issues still associated with random selection vs by-quality. James -- James Bonfield (j...@sanger.ac.uk) The Sanger Institute, Hinxton, Cambs, CB10 1SA -- The Wellcome Sanger Institute is operated by Genome Research Limited, a charity registered in England with number 1021457 and a company registered in England with number 2742969, whose registered office is 215 Euston Road, London, NW1 2BE. _______________________________________________ Samtools-help mailing list Samtools-help@lists.sourceforge.net https://lists.sourceforge.net/lists/listinfo/samtools-help