[ccp4bb] Postdoctoral position in the Lee lab at Duke University School of Medicine
A NIH-funded postdoctoral research position is open in the laboratory of Dr. Seok-Yong Lee at Duke University School of Medicine. The successful candidate will have the opportunity to explore the molecular-level mechanisms of the machinery that underpin membrane transport processes that are critical to human health. The Lee Lab's research is currently centered around three main areas of membrane transport: calcium permeation in somatosensation, drug/metabolite transport, and polysaccharide transport in microbial cell wall synthesis. The types of questions we ask are: what is the molecular basis of temperature and irritant sensing and why is mint cooling and wasabi spicy? How do fungi and bacteria construct their intricate cell walls, and how can these mechanisms be targeted by drugs? How are nutrients and drugs taken up into cells, and how can we improve drug specificity and potency? To probe these questions the lab employs a diverse range of techniques, including cryo-electron microscopy (cryo-EM), biochemistry, biophysics, chemical biology, and computational approaches. Recent notable publications from the lab include: - Yin et al., Science, 2022 - Wright, Fedor, et al., Nature, 2022 - Ren et al., Nat. Struct. & Mol. Biol., 2022 - Kwon et al., Nat. Commun., 2022 - Kwon et al., Nat. Struct. & Mol. Biol., 2021 For more information, visit the Lee Lab website: https://sites.duke.edu/leelab/ This position presents an exceptional opportunity for skilled biochemists or structural biologists with an interest in connecting structural biology to membrane biology. Candidates will benefit from joining a diverse and highly collaborative research group with excellent training opportunities and research facilities. A strong background in either cryo-EM or membrane protein biochemistry is advantageous, but not mandatory. To apply, please submit the following materials to seok-yong@duke.edu: - Curriculum vitae or resume including a list of publications and contact information for three references - Statement of research interests Durham, home to Duke University, is consistently ranked as one of the best places to live in the United States with lush countryside and driving distance to the Atlantic Ocean and Appalachian Mountains. For more information on the city, consult the recent U.S. News and World Report rating: https://realestate.usnews.com/real-estate/slideshows/25-best-places-to-live-in-the-us To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
[ccp4bb] Postdoc position at Duke University
Dear all, We are recruiting a postdoc. A NIH-funded post-doctoral research position is available in the laboratory of Seok-Yong Lee at the Duke University School of Medicine to study the structural and mechanistic basis of membrane transport processes. The lab’s current research focuses on three membrane transport processes: Ca2+ permeation in somatosensation, drug/metabolite transport, and polysaccharide transport in microbial cell wall synthesis. The Lee Lab applies a broad multidisciplinary approach to examine these processes using structural, biochemical, biophysical, chemical biology, and computational methods. The successful applicant will work on advancing our mechanistic understanding of somatosensation into broader biological contexts. Recent publications from the lab are Yin et al., *Science*, 2022 <https://www.science.org/doi/10.1126/science.add1268> Wright, Fedor, et al., *Nature*, 2022 <https://www.nature.com/articles/s41586-022-05168-0> Ren et al., *Nat. Struct. & Mol. Biol.* 2022 <https://www.nature.com/articles/s41594-022-00791-x> Kwon et al., *Nat. Communs.* 2022 <https://www.nature.com/articles/s41467-022-30602-2> Kwon et al., *Nat. Struc. & Mol. Biol*. 2021 <https://www.nature.com/articles/s41594-021-00616-3> Suo et al., *Neuron*, 2020 <https://doi.org/10.1016/j.neuron.2019.11.023> Yin et al., *Science*, 2019 <https://doi.org/10.1126/science.aav9334> Further information can be found on the Lee lab website ( https://sites.duke.edu/leelab/). This is a great opportunity for talented biochemists/structural biologists interested in linking structural biology to sensory neuroscience who would enjoy being embedded in a scientifically diverse and highly collaborative group. A strong background in either structural biology (cryo-EM or X-ray) or membrane protein biochemistry is a plus, but not required. Interested candidates should send their application material, including CV/resume, list of publications, a statement of research interests as well as the contact information of three references, to seok-yong@duke.edu To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
[ccp4bb] Postdoc opportunity
Dear colleagues, Please pass this ad along any one who might be interested. Seok-Yong Applications are invited for a postdoctoral fellow position in the laboratory of Seok-Yong Lee at Duke University School of Medicine to work on the structure and mechanisms of ion channels and transporters. Please see our web page for more details on our research program: https://sites.duke.edu/leelab/ <https://sites.duke.edu/leelab/> Candidates must have obtained a Ph.D. degree within a year and possess a strong background in protein biochemistry (protein expression and purification) and structural biology (single particle cryo-electron microscopy or X-ray crystallography). Additional expertise in membrane protein biochemistry is a plus point. Strong verbal and written communication skills are highly desirable. The Lee lab is fully equipped for structural studies of membrane proteins and has frequent access to the 300 kV Titan Krios TEM with K3 and Falcon III detectors in the Duke University. If you are interested, please email your curriculum vitae, a cover letter describing your research experience and interests and contact information for 2 references to seok-yong@duke.edu <mailto:seok-yong@duke.edu> To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
[ccp4bb] Postdoctoral position in membrane protein structural biology
Dear colleagues, Please pass this ad along any one who might be interested. Seok-Yong Applications are invited for a postdoctoral fellow position in the laboratory of Seok-Yong Lee at Duke University School of Medicine to work on the structure and mechanisms of ion channels and transporters. Please see our web page for more details on our research program: https://sites.duke.edu/leelab/ <https://sites.duke.edu/leelab/> Candidates must have obtained a Ph.D. degree within the past three years and possess a strong background in protein biochemistry (protein expression and purification) and structural biology (single particle cryo-electron microscopy or X-ray crystallography) and be highly organized. Additional expertise in either membrane protein biochemistry or cryo-EM are plus. Strong verbal and written communication skills are highly desirable. The Lee lab is fully equipped for structural studies of membrane proteins and has frequent access to the 300 kV Titan Krios TEM with K3 and Falcon III detectors in the Duke University. If you are interested, please email your curriculum vitae, a cover letter describing your research experience and interests and contact information for 2 references to seok-yong@duke.edu To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
[ccp4bb] local coordinate uncertainty / SFCHECK questions
Hi All, We have recently solved several structures of a membrane protein in slightly different conformations at low resolutions (3.9-4.2 A). We would like to see that these structures reflect truly different conformations and to what extent these structures are discernible. To answer this question, we would need to estimate local and global coordinate uncertainty of these structures to see if the local/global conformational differences of the structures are bigger than the local/global coordinate uncertainty. I am wondering if there is any good way/program to show local coordinate uncertainty. I found that using SFCHECK I can get (i) amplitude of displacement of atom from electron density and (ii) correlation coefficient per residue. I have following questions. 1)Can we use this amplitude of displacement as local coordinate uncertainly? If not, is there a way to use as this displacement amplitude to get an estimate of the local coordinate error? 2)The output regarding this plot in SFCHECK is somewhat difficult to understand, as it shows a bar graph with multiples of sigma per residue. What does it mean by those residues with no sigma? Do these residues have too much or too less errors? 3)Is there a way to convert the output as a text file so that I can plot it myself? 4)Any recommendation with other programs that can produce local coordinate uncertainty per residue? Any advice would be greatly appreciated. Thank you in advance. All the best, Seok-Yong Associate Professor of Biochemistry Duke University School of Medicine USA
