Re: [ccp4bb] determining the domain for overexpression and crystallization

2011-03-17 Thread Xianhui Wu
fide bonds that would certainly help you in > initiating in the right direction. > > > > Best wishes > > Gauri > > > > On Mon, Mar 7, 2011 at 4:10 AM, Xianhui Wu wrote: > > Dear all, > > > > Before we try to study the crystal structure of a

[ccp4bb] determining the domain for overexpression and crystallization

2011-03-07 Thread Xianhui Wu
Dear all, Before we try to study the crystal structure of an unknown protein, we need to determine the sequence that can fold into a compact and stable 3D domain. What kinds of methods can we choose? -- Best regards, XH Wu

[ccp4bb] molecular replacement

2010-06-24 Thread Xianhui Wu
Dear All, I am trying to resolve a protein structure with the use of Molecular Replacement. However, some part of protein are overlap in the interface of homodimer. Would someone please give me suggestions? Thank you! -- Best regards, WuXH

Re: [ccp4bb] Unexplained density

2010-04-29 Thread Xianhui Wu
I think it could be zinc ion. On Fri, Apr 30, 2010 at 5:38 AM, Daniel Bonsor wrote: > Hello again > > I currently have some unexplained density in my structure. As you can > hopefully see from the images (see file), the density is dumbbell shaped. > Whatever it is, it is coordinated by Asp and G