On 12-01-24 08:39 AM, Regina Kettering wrote:
We have a Honeybee system but do not usually use
proteases. The biggest problem we have found is that if
anything precipitates in the tips they have to be washed
very well, usually wit
Hi Horacio,
we have a Cartesian Honeybee and perform trypsin-containing trials by setting
up drops with a dedicated protein+trypsin tip, which we then wash extensively
with water, 6 M guanidinium hydrochloride, then water, isopropanol and again
water.
hth,
ciao,
Sebastiano
On Jan 24, 2012, at
would also
alleviate the protease problem.
Regina
From: Horacio Botti
To: CCP4BB@JISCMAIL.AC.UK
Sent: Tuesday, January 24, 2012 7:12 AM
Subject: [ccp4bb] Crystallization robot and trypsin
Dear all
We may use a Honeybee 963 robot to screen crystallization
Dear all
We may use a Honeybee 963 robot to screen crystallization conditions for
trypsin-containing protein samples and we are worried about robot
contamination by residual protease.
How do you normally clean robots when using this kind of sample? Your
suggestions/recommendations will be appr
