[ccp4bb] Apple X11 10.5
Several people have emailed me about X11 in OS X 10.5. Because I pre- ordered it, I haven't received it yet, so I have only gotten to enjoy the migraines vicariously. Hence, i can't really help. Meanwhile, this looks informative: http://lists.apple.com/archives/X11-users/2007/Oct/msg00065.html
Re: [ccp4bb] X-tallography on OS X aka 10.5 Leopard
Juergen Bosch wrote: > @Bill, > > how about your experience with 10.5 ? Dear Apple Customer, Apple is pleased to report that a shipment for the following order is on its way to you. The following products shipped on 10/26/2007. Product # Product Description QtyExt Price __ MB021Z/A MAC OS X 10.5 RETAIL-INT1 I guess I will know in a few weeks. So much for "pre-ordering." Sort of like "pre-boarding" the aircraft. It will still arrive 2 hours late. Bill
[ccp4bb] Is phophorylation possible in E. coli expression system?
Tyrosine phosphorylation has been known in many bacteria since the late 90s. See for example, Ilan, O., Bloch, Y., Frankel, G., Ullrich, H., Geider, K. & Rosenshine, I. (1999). Protein tyrosine kinases in bacterial pathogens are associated with virulence and production of exopolysaccharide. EMBO J 18, 3241–3248. _ Mark A. Saper, Ph.D. Associate Professor of Biological Chemistry Biophysics, University of Michigan 930 N University Ave Ann Arbor MI 48109-1055 U.S.A. [EMAIL PROTECTED](734) 764-3353fax (734) 764-3323 http://www.biochem.med.umich.edu/biochem/research/profiles/saper.html
Re: [ccp4bb] Is phophorylation possible in E. coli expression system?
However, if your protein is not a protein tyrosine kinase, you may check your western condition. Yes, this is an essential control. Use lots of Lambda protein phosphatase to "dephosporylate" your protein and us the resulting material as a negative control in Western. My experience is that phosphorylations of over-expressed proteins in E. coli are always auto-phosphorylation. Should be the case when there is tons of overexpression. But to be fair, bacteria (and E.coli) do possess tyrosine kinases. It's still possible that one of them does work on some sites found in your protein. Dima
[ccp4bb] Summary: Is phophorylation possible in E. coli expression system?
Dear All: Thanks for all your replies to this. Phosphorylation is possible in E. coli. One article of particular interest is Mol Cell Proteomics 2007 Eprint by Macek B, Gnad, F, Soufu B, Kumar C, Olsen JV, Mijakovic, I and Mann M. Many others have pointed out that it is possible and many likely residues such as Asp, Thr/Ser, His and Tyr are candidates for phosphorylation. To those who wanted to know whether the protein I am working on is a kinase: No, it is not. Thank you all again Subbu
[ccp4bb] X-tallography on OS X aka 10.5 Leopard
Hi all, just a quick draft report on 10.5 and our beloved programs. Upgraded an existing 10.4.10 ( by the way you will need ~5 GB to upgrade your existing system), no clean install. Coot 0.31 does not work (need to upgrade anyway, more sometime this weekend, seems to have troubles with libtiff.3.dylib) ccp4i 6.0.x works ccp4 works (tested just some stuff which was hanging out on my local disc with appropriate data e.g. Refmac, DM, Phaser, Molrep, Arp/Warp) Pymol works Dino works Sharp not tested yet XDS works CNS 1.1 does not work yet hkl2map as front end to ShelX does not work correctly, shelx by itself seems to work OK, need to check this The Uppsala suite works fine The usual stuff, Word, Endnote,Kaleidagraph, Ragtime, Keynote, Excel, Filemaker works Fink, not tried yet Except of having a weird behaviour of X11 e.g copy & paste with middle mouse button emulation via Alt+mousebutton does not work, don't have an external mouse around to test if the real middle button actually works. But in Pymol using the trackpad only, works fine, no differences, so I guess something is broken with my X11. @Bill, how about your experience with 10.5 ? Other than that it's snappier and fancier, Jürgen -- Jürgen Bosch University of Washington Dept. of Biochemistry, K-426 1705 NE Pacific Street Seattle, WA 98195 Box 357742 Phone: +1-206-616-4510 FAX: +1-206-685-7002 Web: http://faculty.washington.edu/jbosch
