Dear Adam and colleagues The enzyme human glutathione reductase (hGR) binds dyes such as safranin and xanthene, which are analogous to methylene blue (aka IZIT) with respect to their heterocyclic ring structures, in a cavity deep at the dimer interface. Safranin and xanthene are also inhibitors of hGR and crystal structures of complexes of GR with safranin and xanthene have been reported: Karplus et al. 1989. Eur J Biochem. 178(3):693-703. Savvides & Karplus 1996. Journal of Biological Chemistry 271(14):8101-7.
Given the general resemblance of these compounds to methylene blue and just to satisfy my curiosity at the time, I soaked methylene-blue into crystals of GR and collected good data to 2 angs. I was basically able to confirm that methylene blue bound specifically in the cavity at the dimer interface just like the other two compounds. However, this work has remained unreported. best regards Savvas On 11 Nov 2011, at 05:34, adam andres wrote: > > > Hi crystallographers > > Has anyone actually collected data on a crystal that > has been treated with Izit dye? If so did you > see any structural effects or know any structure that present this dye? > > Cheers > > Adam Campos >
