[ccp4bb] Postdoctoral position at the MRC Protein Phosphorylation Ubiquitylation Unit, UK
MRC Protein Phosphorylation and Ubiquitylation Unit, Dundee, UK Postdoctoral Scientist Position (Reference number: LS0323) A postdoctoral position is available to study the structural basis of how different polyubiquitin modifications are used as distinct cellular signals. Using X-ray crystallography and other structural methods you will study the molecular mechanisms underlying the decoding of different ubiquitin signals and how signalling enzymes are regulated by ubiquitylation. Broadly, the lab uses an interdisciplinary approach to understand the underlying biochemical and structural mechanisms in ubiquitin signalling, ultimately to discover new signalling nodes and pathways regulated by different ubiquitin modifications. I am looking for an enthusiastic and highly motivated individual with experience in protein crystallography. You should have a PhD in a relevant subject and a desire to understand mechanisms of signal transduction and regulation by post translational modifications. This position is available for 3 years and the salary will be on the University of Dundee Grade 7 scale (£29,541 - £36,298). To apply for this position please visit: http://www.lifesci.dundee.ac.uk/vacancies For more information about the position and informal enquiries contact me (Yogesh Kulathu) y.kula...@dundee.ac.ukmailto:y.kula...@dundee.ac.uk Best wishes Yogesh Yogesh Kulathu MRC Protein Phosphorylation Ubiquitylation Unit University of Dundee College of Life Sciences The Sir James Black Centre Dow Street Dundee DD1 5EH Scotland Office:+44 1382 388163 Lab:+44 1382 3886401 Fax:+44 1382 223778 Website: http://www.ppu.mrc.ac.uk/research/?pid=1001 The University of Dundee is a registered Scottish Charity, No: SC015096
[ccp4bb] Postdoctoral position at The Institute of Cancer Research, Chelsea - London
Following on a BBSRC New Investigator Award, a post-doctoral position is available immediately in the laboratory of Dr. Alessandro Vannini, Division of Structural Biology at The Institute of Cancer Research in London (www.icr.ac.uk/alessandrovannini). We are looking for highly motivated and creative individuals with a strong interest in structural characterization of large multi-subunit complexes. We currently employ single particle electron microscopy analysis and x-ray crystallography, as well as biochemical and biochemical analysis, to elucidate the role of the RNA Polymerase III transcription apparatus in cancer and other fundamental cellular processes. The Division of Structural Biology has managed facilities for protein crystallography (Bruker Microstar and CCD detector and crystallisation robots), cryo-electron microscopy (FEI Tecnai F20 and T12), and protein production with expertise in multi-subunit expression (insect cell, yeast and bacterial expression, including a 60 L fermentor). The Division is also well equipped with equipment for biophysical analysis (e.g. ITC, fluorescence, multi-angle light scattering). Applicants should possess a PhD (or equivalent) in biochemistry or molecular biology with a sound knowledge of multi-subunit complex production and purification. Experience in purification of multi-subunit protein complexes and yeast genetics is essential for this post. Experience in protein crystallography and/or single particle electron microscopy and protein production in insect cells (MultiBac) would be desirable, but individuals with a strong background in yeast genetics and/or biochemistry of multi-subunit complexes wishing to learn structural biology techniques will be taken into serious consideration. Appointment will be on Fixed Term Contract for 2 years in the first instance, with a starting salary in the range of £28,087 to £33,318 p.a. inclusive (based on previous post-doctoral experience). Informal enquiries to alessandro.vann...@icr.ac.uk and applications must be submitted in line with the instructions on our website: http://www.icr.ac.uk/jobs/index.shtml, quoting reference 1336320. Closing date June 20th 2013. Dr. Alessandro Vannini Team Leader, Division of Structural Biology The Institute of Cancer Research Chester Beatty Laboratories 237 Fulham Road London, SW3 6JB United Kingdom
[ccp4bb] Post-doc position available at University of Texas Medical Branch
A post-doctoral position is available in Dr. Kay Choi’s laboratory at the University of Texas Medical Branch for either an X-ray crystallographer or a Biochemist to study the structure and mechanism of viral replication complexes. We use a combination of X-ray crystallography, cryo-electron microscopy, protein chemistry, and bioinformatics to determine the structure of viral replication machinery and to develop antiviral therapeutics. The X-ray crystallography resources include two X-ray area detector systems, a Phoenix crystallization robot, a Minstrel crystal imaging robot, and an Alchemist liquid handling robot. The cryoEM facility includes two JOEL 200 keV cryoEM microscopes for high-resolution imaging work, FEI vitrobot, and a BSL-3 room for viral and pathogen work. Applicants should possess a PhD in structural biology or biochemistry with a sound knowledge of protein purification. Individual with a biochemistry background who wishes to gain expertise in structural biology is encouraged to apply. Interested parties should send a cover letter, a CV and a list of 2-3 references to Dr. Kyung (Kay) Choi via e-mail at kyc...@utmb.edumailto:kyc...@utmb.edu. Kay Choi Assistant Professor Department of Biochemistry and Molecular Biology 6.614C Basic Science phone : 409-747-1402 The University of Texas Medical Branch fax : 409-747-1404 301 University Blvd. email : kay.c...@utmb.edumailto:kay.c...@utmb.edu Galveston, TX 77555-0647
[ccp4bb] how to solve crystal structure using published EM structure
Hi All, The EM structure of a complex was published at 8A. If we can collect crystal data of this complex. How to get the phase of this crystal with the EM structure? Thank you. Sincerely, Lisa
Re: [ccp4bb] how to solve crystal structure using published EM structure
You can use it as an MR model in Molrep for example. Or are you asking where to get the actual EM data from ? That would be here: http://www.ebi.ac.uk/pdbe/emdb/ Jürgen On May 18, 2013, at 8:32 PM, LISA wrote: Hi All, The EM structure of a complex was published at 8A. If we can collect crystal data of this complex. How to get the phase of this crystal with the EM structure? Thank you. Sincerely, Lisa .. Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-2926 http://lupo.jhsph.edu
