Hi Rana,
you are almost there. For the first one I'd add (if at all) 5% PEG200
and for the second I'd mix in 10% PEG200.
Only very big crystals might need even more cryoprotectant.
Best, Matthias
-
Dr. Matthias Zebisch
Division of Structural Biology,
Wellcome Trust Centre for Human Genetics,
University of Oxford,
Roosevelt Drive,
Oxford OX3 7BN, UK
Phone (+44) 1865 287549;
Fax (+44) 1865 287547
Email matth...@strubi.ox.ac.uk
Website http://www.strubi.ox.ac.uk
-
On 2/8/2014 12:44 PM, rana ibd wrote:
Dear CCP4
Does anyone know a good cryoprotection condition for these two
conditions I would be grateful
The first condition:
10% PEG 2, 20%PEG MME 550. 0.03M of NPS ( NPS is sodium nitrate,
disodium hydrogen phosphate, and ammonium sulfate), 0.1MMES/Imadizole
pH 6.5
The second condition:
20% PEG 3350, 0.2M sodium iodide or 0.2M sodium acetate
Thank you
Rana
