Re: [ccp4bb] high purity imidazole
https://www.jiscmail.ac.uk/cgi-bin/webadmin?A2=ind1308L=ccp4bbP=R2552751=ccp4bb9=AJ=ond=No+Match%3BMatch%3BMatchesz=4 Includes updated table. BR -Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Oganesyan, Vaheh Sent: Dienstag, 11. Februar 2014 22:52 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] high purity imidazole Colleagues, In August of 2013 there was a tread regarding high purity Imidazole and someone posted list of products with absorption values at 280 nm and manufacturer. I can find most of the e-mails from that tread but not the one with list of Imidazoles. Has anyone saved that e-mail for future use and willing to share it with me? Vaheh Oganesyan, PhD Antibody development and protein engineering 1 MedImmune Way, Gaithersburg, MD 20878 www.medimmune.com To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation.
Re: [ccp4bb] off-topic Electron density map rendering..
-BEGIN PGP SIGNED MESSAGE- Hash: SHA1 Appalling if the program name is not mentioned in the manuscript. Best, Tim On 02/12/2014 03:08 AM, Francis Reyes wrote: Anyone know the program used to render the electron density maps for the fungal FAS in Figure 3c-3h from the paper Mueller, M., Jenni, S. Ban, N. Strategies for crystallization and structure determination of very large macromolecular assemblies. Curr Opin Struct Biol 17, 572–579 (2007).. The depth cueing is phenomenal. I'm thinking O.. I can't seem to get it right in Pymol. Thanks!, F - Francis E. Reyes PhD 215 UCB University of Colorado at Boulder Publications Citations: http://scholar.google.com/citations?user=FW4-CaQJhl=en -- - -- - -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A -BEGIN PGP SIGNATURE- Version: GnuPG v1.4.12 (GNU/Linux) Comment: Using GnuPG with Icedove - http://www.enigmail.net/ iD8DBQFS+zxcUxlJ7aRr7hoRArIAAJ9FUnn373PWm3uxef4x5LdivxfhBgCfawkH oE12Z6GXrEAAWV2UJKpKDYM= =b53q -END PGP SIGNATURE-
[ccp4bb] I/sigmaI or I/sigmaI
Dear all, Does the I/sigmaI in “Table 1” mean for I/sigmaI or I/sigmaI ? Thanks for your answer. Best wishes, Qixu Cai
Re: [ccp4bb] I/sigmaI or I/sigmaI
I/sigmaI On 12 Feb 2014, at 11:43, Cai Qixu caiq...@gmail.com wrote: Dear all, Does the I/sigmaI in “Table 1” mean for I/sigmaI or I/sigmaI ? Thanks for your answer. Best wishes, Qixu Cai
Re: [ccp4bb] off-topic Electron density map rendering..
You're absolutely right Tim, but unfortunately that's very common these days not to mention the rendering program and ignore the efforts of those who developed it. Boaz Boaz Shaanan, Ph.D. Dept. of Life Sciences Ben-Gurion University of the Negev Beer-Sheva 84105 Israel E-mail: bshaa...@bgu.ac.il Phone: 972-8-647-2220 Skype: boaz.shaanan Fax: 972-8-647-2992 or 972-8-646-1710 From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Tim Gruene [t...@shelx.uni-ac.gwdg.de] Sent: Wednesday, February 12, 2014 11:18 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] off-topic Electron density map rendering.. -BEGIN PGP SIGNED MESSAGE- Hash: SHA1 Appalling if the program name is not mentioned in the manuscript. Best, Tim On 02/12/2014 03:08 AM, Francis Reyes wrote: Anyone know the program used to render the electron density maps for the fungal FAS in Figure 3c-3h from the paper Mueller, M., Jenni, S. Ban, N. Strategies for crystallization and structure determination of very large macromolecular assemblies. Curr Opin Struct Biol 17, 572–579 (2007).. The depth cueing is phenomenal. I'm thinking O.. I can't seem to get it right in Pymol. Thanks!, F - Francis E. Reyes PhD 215 UCB University of Colorado at Boulder Publications Citations: http://scholar.google.com/citations?user=FW4-CaQJhl=en -- - -- - -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A -BEGIN PGP SIGNATURE- Version: GnuPG v1.4.12 (GNU/Linux) Comment: Using GnuPG with Icedove - http://www.enigmail.net/ iD8DBQFS+zxcUxlJ7aRr7hoRArIAAJ9FUnn373PWm3uxef4x5LdivxfhBgCfawkH oE12Z6GXrEAAWV2UJKpKDYM= =b53q -END PGP SIGNATURE-
Re: [ccp4bb] off-topic Electron density map rendering..
I believe that has been rendered in O, selecting a different line drawing mode you could get by hitting 'F3' a couple of times, IIRC. On 12 February 2014 12:05, Boaz Shaanan bshaa...@bgu.ac.il wrote: You're absolutely right Tim, but unfortunately that's very common these days not to mention the rendering program and ignore the efforts of those who developed it. Boaz Boaz Shaanan, Ph.D. Dept. of Life Sciences Ben-Gurion University of the Negev Beer-Sheva 84105 Israel E-mail: bshaa...@bgu.ac.il Phone: 972-8-647-2220 Skype: boaz.shaanan Fax: 972-8-647-2992 or 972-8-646-1710 From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Tim Gruene [t...@shelx.uni-ac.gwdg.de] Sent: Wednesday, February 12, 2014 11:18 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] off-topic Electron density map rendering.. -BEGIN PGP SIGNED MESSAGE- Hash: SHA1 Appalling if the program name is not mentioned in the manuscript. Best, Tim On 02/12/2014 03:08 AM, Francis Reyes wrote: Anyone know the program used to render the electron density maps for the fungal FAS in Figure 3c-3h from the paper Mueller, M., Jenni, S. Ban, N. Strategies for crystallization and structure determination of very large macromolecular assemblies. Curr Opin Struct Biol 17, 572-579 (2007).. The depth cueing is phenomenal. I'm thinking O.. I can't seem to get it right in Pymol. Thanks!, F - Francis E. Reyes PhD 215 UCB University of Colorado at Boulder Publications Citations: http://scholar.google.com/citations?user=FW4-CaQJhl=en -- - -- - -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A -BEGIN PGP SIGNATURE- Version: GnuPG v1.4.12 (GNU/Linux) Comment: Using GnuPG with Icedove - http://www.enigmail.net/ iD8DBQFS+zxcUxlJ7aRr7hoRArIAAJ9FUnn373PWm3uxef4x5LdivxfhBgCfawkH oE12Z6GXrEAAWV2UJKpKDYM= =b53q -END PGP SIGNATURE- -- Dr Jon Agirre York Structural Biology Laboratory / Department of Chemistry University of York, Heslington, YO10 5DD, York, England http://www.york.ac.uk/chemistry/research/ysbl/people/research/jagirre/ +44 (0) 1904 32 8253
[ccp4bb] *conference reminder* 3rd annual CCP-BioSim conference - Frontiers of Biomolecular Simulation - University of Edinburgh - 21st-23rd May 2014
As advertised at the Study Weekend, CCPBioSim is the sister CCP in biomolecular simulation. The annual conference will take place in Edinburgh in May, see details below. Cheers Martyn Dear Colleagues, The third annual CCP-BioSim conference will be held at the John McIntyre conference centre, Edinburgh First, from Wednesday 21st to Friday 23rd May 2014. The aim of the event is to bring together computational and experimental scientists with an interest in developing and applying biomolecular simulation techniques to their work. The deadline for submission of abstracts for a contributed oral or poster presentation is March the 10th. Further information regarding registration, schedule, abstract submission and other matters is available at: http://www.ccpbiosim.ac.uk/?q=annualconfs/conf2014 Confirmed Invited Speakers * Alexandre Bonvin, Bijvoet Center for Biomolecular Research, Utrecht, Netherlands * Cecilia Clementi, Rice University, Department of Chemistry, USA * Michael Gilson, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, USA * Bert de Groot, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany * Frauke Gräter, Heidelberg Institute of Theoretical Studies, Germany * Gerhard Hummer, Max Planck Institute of Biophysics, Frankfurt am Main, Germany * Michael Mazanetz, Evotec UK Ltd. , UK * Irina Tikhonova, School of Pharmacy, Queen's University Belfast, UK -- Dr. Julien Michel, Royal Society University Research Fellow Room 263 School of Chemistry University of Edinburgh West Mains Road Edinburgh, EH9 3JJ United Kingdom phone: +44 (0)131 650 4797tel:%2B44%20%280%29131%20650%204797 http://www.julienmichel.net/ - -- Scanned by iCritical.
Re: [ccp4bb] high purity imidazole
Thanks to Debanu Das, Pedro J. B. Pereira and Bernhard Rupp for pointing me in the right direction. https://www.jiscmail.ac.uk/cgi-bin/webadmin?A2=ind1308L=ccp4bbP=R2552751=ccp4bb9=AJ=ond=No+Match%3BMatch%3BMatchesz=4 Regards, Vaheh Oganesyan www.medimmune.com To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation.
Re: [ccp4bb] Sister CCPs
It would be so nice to have a 'sister CCP' for questions aboud wet-lab problems that have nothing to do with CCP4 or crystallographic computing, The is clearly a big need for it, and those of us who try to keep out of wet-labs would not have to wade though it all. George On 02/12/2014 03:05 PM, Martyn Winn wrote: As advertised at the Study Weekend, CCPBioSim is the sister CCP in biomolecular simulation. The annual conference will take place in Edinburgh in May, see details below. Cheers Martyn -- Prof. George M. Sheldrick FRS Dept. Structural Chemistry, University of Goettingen, Tammannstr. 4, D37077 Goettingen, Germany Tel. +49-551-39-33021 or -33068 Fax. +49-551-39-22582
[ccp4bb] Workshop - Biocrystallography for the high-throughput era -July 1-5, 2014, Bialystok, Poland
Dear Colleagues, I would like to announce a workshop on Biocrystallography for the high-throughput era. The workshop is addressed to students, postdocs and young researchers, who are engaged in structural studies of macromolecules. The aim is to familiarize the participants with modern methods of biocrystallography as applied to structural studies of proteins and their complexes, including bioinformatics approaches. The workshop will consist of hands-on practical sessions supported by lectures. All students are encourage to bring their own protein or diffraction data. Additionally, a poster session will be held during the workshop, and the participants are encourage to present their own research projects. The workshop will take place in the Institute of Chemistry, University of Bialystok and 3Trio Hotel, starting on July 1st, 2014, and ending on July 5th. Application deadline: March 15, 2014 The topics will include: * bioinformatics approaches prior to crystallographic studies * high-throughput protein expression, purification and crystallization * efficient X-ray diffraction data collection and processing * phasing and structure determination * model refinement and validation Confirmed Invited Speakers and Tutors: * Matthias Bochtler, International Institute of Molecular and Cell Biology, Warsaw, PL * Zbyszek Dauter, National Cancer Institute, Argonne, USA * Miroslaw Gilski, A. Mickiewicz University, Poznan, PL * Mariusz Jaskolski, A. Mickiewicz University, Poznan, PL * Robert Jedrzejczak, Midwest Center for Structural Genomics, Argonne, USA * Wladek Minor, University of Virginia, Charlottesville, USA * Boguslaw Nocek, Midwest Center for Structural Genomics, Argonne, USA Detailed information can be found on the workshop web page: http://biocrystallography.org.pl We look forward to welcoming you to Bialystok Dr. Krzysztof Brzezinski Institute of Chemistry University of Bialystok Hurtowa 1 15-399 Bialystok Poland
Re: [ccp4bb] I/sigmaI or I/sigmaI
How did people get I/sigmI when using HKL2000? Ron On Wed, 12 Feb 2014, Phil Evans wrote: I/sigmaI On 12 Feb 2014, at 11:43, Cai Qixu caiq...@gmail.com wrote: Dear all, Does the I/sigmaI in “Table 1” mean for I/sigmaI or I/sigmaI ? Thanks for your answer. Best wishes, Qixu Cai
Re: [ccp4bb] I/sigmaI or I/sigmaI
Phil Jeffrey has a small program to calculate avg. I/sig for Table 1 including for resolution ranges. Jackie Vitali On Wed, Feb 12, 2014 at 11:20 AM, Ronald E Stenkamp stenk...@u.washington.edu wrote: How did people get I/sigmI when using HKL2000? Ron On Wed, 12 Feb 2014, Phil Evans wrote: I/sigmaI On 12 Feb 2014, at 11:43, Cai Qixu caiq...@gmail.com wrote: Dear all, Does the I/sigmaI in Table 1 mean for I/sigmaI or I/sigmaI ? Thanks for your answer. Best wishes, Qixu Cai
[ccp4bb] השב: Re: [ccp4bb] Sister CCPs
Dear George, I'm not sure it's a good idea since the wet work and coputational work are quite often linked. For example preparation of heavy atom derivatives and handling the data, to which bb would you send questions related to this subject? To both bb's? Wouldn't that make life even more complicated in terms of handling morebb's. Just wondering. Cheers, Boaz הודעה מקורית מאת George Sheldrick gshe...@shelx.uni-ac.gwdg.de תאריך: 12/02/2014 18:03 (GMT02:00) אל CCP4BB@JISCMAIL.AC.UK נושא Re: [ccp4bb] Sister CCPs It would be so nice to have a 'sister CCP' for questions aboud wet-lab problems that have nothing to do with CCP4 or crystallographic computing, The is clearly a big need for it, and those of us who try to keep out of wet-labs would not have to wade though it all. George On 02/12/2014 03:05 PM, Martyn Winn wrote: As advertised at the Study Weekend, CCPBioSim is the sister CCP in biomolecular simulation. The annual conference will take place in Edinburgh in May, see details below. Cheers Martyn -- Prof. George M. Sheldrick FRS Dept. Structural Chemistry, University of Goettingen, Tammannstr. 4, D37077 Goettingen, Germany Tel. 49-551-39-33021 or -33068 Fax. 49-551-39-22582
Re: [ccp4bb] I/sigmaI or I/sigmaI
phenix.merging_statistics gives it also. And I think a recent post by Z.O. indicated the current version of the HKL suite gives I/sig(I) and new statistics including CC1/2 or CC*. Jacqueline Vitali wrote: Phil Jeffrey has a small program to calculate avg. I/sig for Table 1 including for resolution ranges. Jackie Vitali On Wed, Feb 12, 2014 at 11:20 AM, Ronald E Stenkamp stenk...@u.washington.edu mailto:stenk...@u.washington.edu wrote: How did people get I/sigmI when using HKL2000? Ron On Wed, 12 Feb 2014, Phil Evans wrote: I/sigmaI On 12 Feb 2014, at 11:43, Cai Qixu caiq...@gmail.com mailto:caiq...@gmail.com wrote: Dear all, Does the I/sigmaI in “Table 1” mean for I/sigmaI or I/sigmaI ? Thanks for your answer. Best wishes, Qixu Cai
Re: [ccp4bb] השב: Re: [ccp4bb] Sister CCPs
George, Crystallization, cryoprotection are wet but important steps before data collection. Which topics would you like to exclude? Enrico. On Wed, 12 Feb 2014 17:32:47 +0100, Boaz Shaanan bshaa...@bgu.ac.il wrote: Dear George, I'm not sure it's a good idea since the wet work and coputational work are quite often linked. For example preparation of heavy atom derivatives and handling the data, to which bb would you send questions related to this subject? To both bb's? Wouldn't that make life even more complicated in terms of handling more bb's. Just wondering. Cheers, Boaz הודעה מקורית מאת George Sheldrick gshe...@shelx.uni-ac.gwdg.de תאריך: 12/02/2014 18:03 (GMT+02:00) אל CCP4BB@JISCMAIL.AC.UK נושא Re: [ccp4bb] Sister CCPs It would be so nice to have a 'sister CCP' for questions aboud wet-lab problems that have nothing to do with CCP4 or crystallographic computing, The is clearly a big need for it, and those of us who try to keep out of wet-labs would not have to wade though it all. George On 02/12/2014 03:05 PM, Martyn Winn wrote: As advertised at the Study Weekend, CCPBioSim is the sister CCP in biomolecular simulation. The annual conference will take place in Edinburgh in May, see details below. Cheers Martyn -- Enrico A. Stura D.Phil. (Oxon) ,Tel: 33 (0)1 69 08 4302 Office Room 19, Bat.152, Tel: 33 (0)1 69 08 9449Lab http://www-dsv.cea.fr/ibitecs/simopro/ltmb/cristallogenese LTMB, SIMOPRO, IBiTec-S, CE Saclay, 91191 Gif-sur-Yvette, FRANCE http://scholar.google.com/citations?hl=enuser=Kvm06WIoPAsCpagesize=100sortby=pubdate http://www.chem.gla.ac.uk/protein/mirror/stura/index2.html e-mail: est...@cea.fr Fax: 33 (0)1 69 08 90 71
Re: [ccp4bb] Sister CCPs
Don't know... I'm quite happy to wade through the wet stuff, keeps me in touch with the stuff that people actually struggle with. On 12/02/2014 15:59, George Sheldrick wrote: It would be so nice to have a 'sister CCP' for questions aboud wet-lab problems that have nothing to do with CCP4 or crystallographic computing, The is clearly a big need for it, and those of us who try to keep out of wet-labs would not have to wade though it all. George On 02/12/2014 03:05 PM, Martyn Winn wrote: As advertised at the Study Weekend, CCPBioSim is the sister CCP in biomolecular simulation. The annual conference will take place in Edinburgh in May, see details below. Cheers Martyn
Re: [ccp4bb] Sister CCPs
On 12/02/14 15:59, George Sheldrick wrote: It would be so nice to have a 'sister CCP' for questions aboud wet-lab problems that have nothing to do with CCP4 or crystallographic computing, The is clearly a big need for it, and those of us who try to keep out of wet-labs would not have to wade though it all. FWIW, the remit of CCP4BB, held at jiscmail-central, is describes as: /The CCP4BB mailing list is for discussions on the use of the CCP4 suite, and macromolecular crystallography in general./ Thus wet-lab questions are not off-topic (not that anyone recently described them as such). Having said that, Jiscmail mailing lists are easy to set-up (providing that you can reasonably expect that the mailing list will improve knowledge sharing within the UK centered academic community) and relatively low maintenance. I, for one, would not be entirely unhappy to miss out on questions about lysis. Paul.
[ccp4bb] Postdoc Position on Structural Biology of Membrane Proteins in Brazil
Dear colleagues, I intend to implement work on structural biology (by X-ray crystallography) of membrane proteins in my lab, more specifically on the subset of inner mitochondrial membrane carriers. For this, I would like to take this opportunity and invite anybody who is prospecting a postdoc in the field, and for the second semester of 2014, to consider coming to Brazil. My laboratory is located at the Brazilian National Laboratory for Biosciences (LNBio), which is hosted in the same campus as the Brazilian Synchrotron Light Source (LNLS) as well as the third-generation synchrotron source Sirius, currently under construction. LNBio has an exceptional infrastructure dedicated to all aspects of structural biology. The campus is located in Campinas, about 1 hour driving, inland from the city of São Paulo. Brazilian research grants to principal investigators seldom allow the payment of salaries to postdocs or students, so as a rule fellowships or scholarships have to be applied on demand to the funding agencies, as in this case. Specifically, the PD fellowship proposal I advertise will be presented - in the form of a research project - to the funding agency which has been funding my lab since its establishment (about 4 years ago), as a joint application by myself and the chosen candidate. The time frame for analysis is around 100 days from submission. If approved, the PD fellowship is granted for 24 months and can be renewed for an extra 12 months. The stipend is currently R$ 5.908,80 or approximately USD 2,500.00, tax-free. According to the agency rules, on demand PD fellowship applications that receive a favorable assessment from ad hoc reviewers will be put forward for a monthly comparative analysis session, which in my experience, takes especially into consideration the previous experience of the candidate in the field; thus, a publication record in structural biology and/or membrane proteins may grant considerable advantage to the candidate. So, if yourself or anybody you know would like to consider this opportunity, please, do not hesitate to contact me, sending a copy of your CV and the name of a reference scientist to whom I could ask for recommendation. I will be more than happy to provide further details on every aspect of this position (including the research project, the fellowship, my institution or myself) to serious, motivated and talented candidates. The funding agency requires that the candidate should have concluded a doctorate less than seven years before the beginning of the PD fellowship, which would be around August or September 2014. Thank you for your attention, Andre LB Ambrosio LNBio, CNPEM - Brazil
Re: [ccp4bb] Sister CCPs
From the point of view of one of the wet lab personnel, picking the brains of the experienced keeps us from reinventing the wheel at intervals. We really appreciate the insight you can offer when we venture into unknown territories, and we are grateful when you take the time to share. I don't think there is an email system in existence that allows anyone to totally screen the incoming mail. Often the content is fairly clear from the subject line for sorting in this system. My thanks, Carol Kolar Carol Kolar, Researcher and Lab Manager LTC 10736 987696 Nebraska Medical Center Omaha, NE 68198-7696 From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Paul Emsley [pems...@mrc-lmb.cam.ac.uk] Sent: Wednesday, February 12, 2014 12:22 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Sister CCPs On 12/02/14 15:59, George Sheldrick wrote: It would be so nice to have a 'sister CCP' for questions aboud wet-lab problems that have nothing to do with CCP4 or crystallographic computing, The is clearly a big need for it, and those of us who try to keep out of wet-labs would not have to wade though it all. FWIW, the remit of CCP4BB, held at jiscmail-central, is describes as: /The CCP4BB mailing list is for discussions on the use of the CCP4 suite, and macromolecular crystallography in general./ Thus wet-lab questions are not off-topic (not that anyone recently described them as such). Having said that, Jiscmail mailing lists are easy to set-up (providing that you can reasonably expect that the mailing list will improve knowledge sharing within the UK centered academic community) and relatively low maintenance. I, for one, would not be entirely unhappy to miss out on questions about lysis. Paul. The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.
Re: [ccp4bb] Sister CCPs
It's not an e-mail bulletin board, but Researchgate seems to be quite popular for wet lab questions. IMO the QA section of the social network is a bit messy. That said, the quality seems to improve gradually. Cheers, Robbie Sent from my Windows Phone Van: Paul Emsley Verzonden: 12-2-2014 19:23 Aan: CCP4BB@JISCMAIL.AC.UK Onderwerp: Re: [ccp4bb] Sister CCPs On 12/02/14 15:59, George Sheldrick wrote: It would be so nice to have a 'sister CCP' for questions aboud wet-lab problems that have nothing to do with CCP4 or crystallographic computing, The is clearly a big need for it, and those of us who try to keep out of wet-labs would not have to wade though it all. FWIW, the remit of CCP4BB, held at jiscmail-central, is describes as: /The CCP4BB mailing list is for discussions on the use of the CCP4 suite, and macromolecular crystallography in general./ Thus wet-lab questions are not off-topic (not that anyone recently described them as such). Having said that, Jiscmail mailing lists are easy to set-up (providing that you can reasonably expect that the mailing list will improve knowledge sharing within the UK centered academic community) and relatively low maintenance. I, for one, would not be entirely unhappy to miss out on questions about lysis. Paul.
[ccp4bb] השב: Re: [ccp4bb] Sister CCPs
The wet lab questions on ccp4bb are crystallography related in most if not all cases, aren't they? I'm quite sure that people send more general wet lab questions to other bb's. Boaz הודעה מקורית מאת Robbie Joosten robbie_joos...@hotmail.com תאריך: 12/02/2014 21:22 (GMT02:00) אל CCP4BB@JISCMAIL.AC.UK נושא Re: [ccp4bb] Sister CCPs It's not an e-mail bulletin board, but Researchgate seems to be quite popular for wet lab questions. IMO the QA section of the social network is a bit messy. That said, the quality seems to improve gradually. Cheers, Robbie Sent from my Windows Phone Van: Paul Emsley Verzonden: 12-2-2014 19:23 Aan: CCP4BB@JISCMAIL.AC.UK Onderwerp: Re: [ccp4bb] Sister CCPs On 12/02/14 15:59, George Sheldrick wrote: It would be so nice to have a 'sister CCP' for questions aboud wet-lab problems that have nothing to do with CCP4 or crystallographic computing, The is clearly a big need for it, and those of us who try to keep out of wet-labs would not have to wade though it all. FWIW, the remit of CCP4BB, held at jiscmail-central, is describes as: /The CCP4BB mailing list is for discussions on the use of the CCP4 suite, and macromolecular crystallography in general./ Thus wet-lab questions are not off-topic (not that anyone recently described them as such). Having said that, Jiscmail mailing lists are easy to set-up (providing that you can reasonably expect that the mailing list will improve knowledge sharing within the UK centered academic community) and relatively low maintenance. I, for one, would not be entirely unhappy to miss out on questions about lysis. Paul.
[ccp4bb] Postdoc position in chromosome cryo-ET at the National University of Singapore
Dear Colleagues, A postdoctoral research fellow position is available in the Centre for BioImaging Sciences at the National University of Singapore, Singapore. The candidate will work with my team to study chromatin in eukaryotic cells using electron cryotomography (cryo-ET). The goal is to determine how chromatin structure couples to transcription, genome integrity, and development. The Centre of BioImaging Sciences is equipped with state-of-the-art instrumentation, including a Titan Krios equipped with a Falcon direct detector. These imaging facilities are supported by excellent staff and computational resources. The candidate should have experience with X-ray crystallography and/or electron microscopy (either biological or materials science). S/he should be highly motivated and must have a strong research record as demonstrated by publications. Interested candidates should send a cover letter, CV, and three recommendation letters to Lu Gan (l...@anaphase.org). Cheers, -Lu -- Lu Gan Assistant Professor Department of Biological Sciences National University of Singapore 14 Science Drive 4 S1A, Lvl 2 Singapore 117543 www.anaphase.org Tel: (65) 6516 8868 Fax: (65) 6776 7882
[ccp4bb] Rmerge of Images
Dear Crystallographers, Where can I find the definition of the R vs batch reported in scaling? Specifically I am wondering whether it is cumulative (each new frame versus all previous ones pooled together) or something else, and also how this metric can have any meaning on early frames when one has measured each reflection only once (in p1, this would be 180 degrees). I am wondering about its efficacy as a radiation-damage metric. JPK *** Jacob Pearson Keller, PhD Looger Lab/HHMI Janelia Farms Research Campus 19700 Helix Dr, Ashburn, VA 20147 email: kell...@janelia.hhmi.org ***
