Re: [ccp4bb] Beamtime @ SLS
Sad, no Eiger. There might be teething problems. Richard On 11.04.2015, at 12:03, Meitian Wang meitian.w...@psi.chmailto:meitian.w...@psi.ch wrote: === SYNCHROTRON BEAM TIME FOR MACROMOLECULAR CRYSTALLOGRAPHY AT SLS === NOTE: Proposal call schedule has been changed from 3-call to 2-call per year. The current call is the first call in the new schedule. Proposal application deadline: Wednesday, April 15, 2015 Periods: July 1, 2015 - December 31, 2015 (Normal / Test proposals) July 1, 2015 - June 30, 2017 (Long-term proposals) Proposal submission: http://www.psi.ch/sls/px-beamlines-call-for-proposals Travel support: http://www.psi.ch/useroffice/sls-elisa-biostruct X06SA Beamline features (http://www.psi.ch/sls/pxi/pxi) - Undulator beamline with flux of 2x10^12 photons/sec at 12.4 keV (1?) and fully tunable from 6.0 to 17.5 keV (2.07 - 0.71 ?) - Focused beam size and divergency: 10x5 microns and 2.0x0.3 mrad - Variable beam size from 5 um to 100 um - In meso in situ serial crystallography with micro-beam and SLS PX software (DA+) - PILATUS 6M pixel detector enabling continuous and fine phi-sliced data acquisition with 20 bit dynamic range (see http://pilatus.web.psi.ch/ or www.dectris.comhttp://www.dectris.com/ for further information) X06DA Beamline features (http://www.psi.ch/sls/pxiii/pxiii) - Super-bending magnet beamline with flux of 5x10^1^1 photons/sec at 12.4 keV (1?) and fully tunable from 6.0 to 17.5 keV (2.07 - 0.71 ?) - Focused beam size and divergency: 80x45 microns and 2x0.5 mrad (with possibility to reduce horizontal divergency to 0.4 mrad) - Mini-hutch design for fast manual mounting - PILATUS 2M (60 Hz) - Multi-axis goniometer (PRIGO) for native-SAD phasing (T. Weinert, et al., Nature Methods, 12, 131, 2015) - New phasing protocols with energy interleaving and multi-orientation strategy - In-situ X-ray diffraction screening (with any SBS format plate) available during users shifts (R. Bingel-Erlenmeyer, et al., Crystal Growth Design 11, 916, 2011) X10SA Beamline features (http://www.psi.ch/sls/pxii/pxii) - Undulator beamline with flux of 2x10^12 photons/sec at 12.4 keV (1?) and fully tunable from 6.0 to 20 keV (2.07 - 0.62 ?) - Focused beam size and divergency: 50x10 microns and 0.6x0.1 mrad - Micro-beam with apertures (10 x 10, 30 x 30 micrometer) - PILATUS 6M pixel detector Best regards, The MX group at SLS __ Meitian Wang Swiss Light Source at Paul Scherrer Institut CH-5232 Villigen PSI - http://www.psi.ch/sls/ Phone: +41 56 310 4175
[ccp4bb] Beamtime @ SLS
=== SYNCHROTRON BEAM TIME FOR MACROMOLECULAR CRYSTALLOGRAPHY AT SLS === NOTE: Proposal call schedule has been changed from 3-call to 2-call per year. The current call is the first call in the new schedule. Proposal application deadline: Wednesday, April 15, 2015 Periods: July 1, 2015 - December 31, 2015 (Normal / Test proposals) July 1, 2015 - June 30, 2017 (Long-term proposals) Proposal submission: http://www.psi.ch/sls/px-beamlines-call-for-proposals Travel support: http://www.psi.ch/useroffice/sls-elisa-biostruct X06SA Beamline features (http://www.psi.ch/sls/pxi/pxi) - Undulator beamline with flux of 2x10^12 photons/sec at 12.4 keV (1Å) and fully tunable from 6.0 to 17.5 keV (2.07 - 0.71 Å) - Focused beam size and divergency: 10x5 microns and 2.0x0.3 mrad - Variable beam size from 5 um to 100 um - In meso in situ serial crystallography with micro-beam and SLS PX software (DA+) - PILATUS 6M pixel detector enabling continuous and fine phi-sliced data acquisition with 20 bit dynamic range (see http://pilatus.web.psi.ch/ or www.dectris.com for further information) X06DA Beamline features (http://www.psi.ch/sls/pxiii/pxiii) - Super-bending magnet beamline with flux of 5x10¹¹ photons/sec at 12.4 keV (1Å) and fully tunable from 6.0 to 17.5 keV (2.07 - 0.71 Å) - Focused beam size and divergency: 80x45 microns and 2x0.5 mrad (with possibility to reduce horizontal divergency to 0.4 mrad) - Mini-hutch design for fast manual mounting - PILATUS 2M (60 Hz) - Multi-axis goniometer (PRIGO) for native-SAD phasing (T. Weinert, et al., Nature Methods, 12, 131, 2015) - New phasing protocols with energy interleaving and multi-orientation strategy - In-situ X-ray diffraction screening (with any SBS format plate) available during users shifts (R. Bingel-Erlenmeyer, et al., Crystal Growth Design 11, 916, 2011) X10SA Beamline features (http://www.psi.ch/sls/pxii/pxii) - Undulator beamline with flux of 2x10^12 photons/sec at 12.4 keV (1Å) and fully tunable from 6.0 to 20 keV (2.07 - 0.62 Å) - Focused beam size and divergency: 50x10 microns and 0.6x0.1 mrad - Micro-beam with apertures (10 x 10, 30 x 30 micrometer) - PILATUS 6M pixel detector Best regards, The MX group at SLS __ Meitian Wang Swiss Light Source at Paul Scherrer Institut CH-5232 Villigen PSI - http://www.psi.ch/sls/ Phone: +41 56 310 4175
Re: [ccp4bb] Problem in optimization
Dear , If you want to get useful advice you have to give a bit more information. What did you try? Also, please do not attach large images to your message. Thanks and good luck, bert From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of 高艺娜 [gaoy...@cau.edu.cn] Sent: Saturday, April 11, 2015 11:00 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Problem in optimization Dear all I have tried a variety of methods on regular optimization of the crystal ,but all have failed,the 17 kDa protein crystal still have many nucleation and poor diffraction also poor resolution(the best 3.5－4.0Å） The crystallization conditions are: 0.1 M Tris pH-6.5, 0.2 M Sodium acetate 1.8 M Ammonium sulphate Could some one suggest me how to trouble shoot this problem? Best wishes,
Re: [ccp4bb] Problem in optimization
Additive screening At 2015-04-11 18:00:21, 高艺娜 gaoy...@cau.edu.cn wrote: Dear all I have tried a variety of methods on regular optimization of the crystal ,but all have failed,the 17 kDa protein crystal still have many nucleation and poor diffraction also poor resolution(the best 3.5－4.0Å） The crystallization conditions are: 0.1 M Tris pH-6.5, 0.2 M Sodium acetate 1.8 M Ammonium sulphate Could some one suggest me how to trouble shoot this problem? Best wishes,
