Re: [ccp4bb] screw axes /system. absenses and phaser/MR solutions

2018-08-08 Thread Eleanor Dodson 
What is the tNCS vector?

Eleanor

On 8 August 2018 at 22:06, Kajander, Tommi A 
wrote:

> Dear Eleanor, no tNCS is present here..(2 mols). I think its probably the
> somewhat incomplete low resolution (90% ish) that causes problems
> somewhere... which again follows from bit poir crystal quality in some
> directions - overall data is not great...
>
> Best,
> Tommi
>
>
> Kohteesta: Eleanor Dodson
> Lähetetty: keskiviikko 8. elokuuta klo 17.46
> Aihe: Re: [ccp4bb] screw axes /system. absenses and phaser/MR solutions
> Vastaanottaja: Kajander, Tommi A
> Kopio: CCP4BB@JISCMAIL.AC.UK
>
>
> How many molecules in the asymmetric unit? and is there a
> non-crystallographic translaton?
>
> Eleanor
>
> On 8 August 2018 at 15:29, Kajander, Tommi A 
> wrote:
>
> Hi,
> Any clues why the followting happens: pointless (and just looking at the
> XDS output) clearly tells there is one screw axis in P-ortorhombic (P2212)
> yet phaser gives the best Z-scores in P22121. (...I suspect this may be to
> do with twinning - might be monoclinic twiined still though now processes
> very well in P222.)
>
> If i run the mtz after XDSCONV (ie F2MTZ) via pointless (instead of
> directly after XDS) it also suggests this - but i suppose i am not suppose
> to run merged data via pointless.
>
> Thanks for comments,
>
> Tommi
>
> 
>
> To unsubscribe from the CCP4BB list, click the following link:
> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
>
>
>
>
>



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Re: [ccp4bb] screw axes /system. absenses and phaser/MR solutions

2018-08-08 Thread Kajander, Tommi A 
Dear Eleanor, no tNCS is present here..(2 mols). I think its probably the 
somewhat incomplete low resolution (90% ish) that causes problems somewhere... 
which again follows from bit poir crystal quality in some directions - overall 
data is not great...

Best,
Tommi


Kohteesta: Eleanor Dodson
Lähetetty: keskiviikko 8. elokuuta klo 17.46
Aihe: Re: [ccp4bb] screw axes /system. absenses and phaser/MR solutions
Vastaanottaja: Kajander, Tommi A
Kopio: CCP4BB@JISCMAIL.AC.UK


How many molecules in the asymmetric unit? and is there a non-crystallographic 
translaton?

Eleanor

On 8 August 2018 at 15:29, Kajander, Tommi A 
mailto:tommi.kajan...@helsinki.fi>> wrote:
Hi,
Any clues why the followting happens: pointless (and just looking at the XDS 
output) clearly tells there is one screw axis in P-ortorhombic (P2212)
yet phaser gives the best Z-scores in P22121. (...I suspect this may be to do 
with twinning - might be monoclinic twiined still though now processes very 
well in P222.)

If i run the mtz after XDSCONV (ie F2MTZ) via pointless (instead of directly 
after XDS) it also suggests this - but i suppose i am not suppose to run merged 
data via pointless.

Thanks for comments,

Tommi



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Re: [ccp4bb] screw axes /system. absenses and phaser/MR solutions

2018-08-08 Thread Eleanor Dodson 
How many molecules in the asymmetric unit? and is there a
non-crystallographic translaton?

Eleanor

On 8 August 2018 at 15:29, Kajander, Tommi A 
wrote:

> Hi,
> Any clues why the followting happens: pointless (and just looking at the
> XDS output) clearly tells there is one screw axis in P-ortorhombic (P2212)
> yet phaser gives the best Z-scores in P22121. (...I suspect this may be to
> do with twinning - might be monoclinic twiined still though now processes
> very well in P222.)
>
> If i run the mtz after XDSCONV (ie F2MTZ) via pointless (instead of
> directly after XDS) it also suggests this - but i suppose i am not suppose
> to run merged data via pointless.
>
> Thanks for comments,
>
> Tommi
>
> 
>
> To unsubscribe from the CCP4BB list, click the following link:
> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
>



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[ccp4bb] screw axes /system. absenses and phaser/MR solutions

2018-08-08 Thread Kajander, Tommi A 
Hi,
Any clues why the followting happens: pointless (and just looking at the XDS 
output) clearly tells there is one screw axis in P-ortorhombic (P2212)
yet phaser gives the best Z-scores in P22121. (...I suspect this may be to do 
with twinning - might be monoclinic twiined still though now processes very 
well in P222.)

If i run the mtz after XDSCONV (ie F2MTZ) via pointless (instead of directly 
after XDS) it also suggests this - but i suppose i am not suppose to run merged 
data via pointless.

Thanks for comments,

Tommi 



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[ccp4bb] Poster Abstract deadline, 17th August: 10th International Workshop on Radiation Damage to Biological Samples. Brookhaven National Laboratory, 13-14th September 2018

2018-08-08 Thread Elspeth Garman 
10th International Workshop on Radiation Damage to Biological Samples. 
Brookhaven National Laboratory, 13-14th September 2018: 
https://www.bnl.gov/rd10/

The Poster Abstract deadline is 17th August.
Come and discuss your results with others interested in Radiation Damage!
Registration for the Workshop closes on 31st August.

The Workshop will address essential questions and challenges of radiation 
damage to biological samples during their examination with ionizing radiation. 
The workshop will cover various X-ray and electron scattering techniques, from 
crystallography to imaging, and offers ample opportunities for information 
exchange and discussion among researchers from around the globe.
Sessions will cover:
1) Basic Understanding of Radiation Damage Mechanisms
2) Biological Studies Affected by Radiation Damage
3) Practical Aspects of Reducing Radiation Damage at Synchrotrons
4) Damage at New Sources - XFEL and 4th generation synchrotrons
5) Radiation Damage in Complementary Fields including Biological Imaging

Best wishes
Elspeth Garman
On behalf of the Organising Committee

Elspeth F. Garman,
Professor of Molecular Biophysics,
Senior Kurti Fellow, Brasenose College, University of Oxford
Postal address:
  Laboratory of Molecular Biophysics,
  Department of Biochemistry,
  University of Oxford,  Tel: (44)-1865-613297
  South Parks Road,  FAX: (44)-1865-613201
  OXFORD, OX1 3QU, U.K. E-mail: 
elspeth.gar...@bioch.ox.ac.uk
http://www.bioch.ox.ac.uk/aspsite/index.asp?sectionid=garman
 -





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[ccp4bb] upcoming VIB conference on Structural Biology

2018-08-08 Thread Savvas Savvides 
Dear colleagues,

I am taking the liberty of bringing to your attention the VIB's bi-annual 
conference on Structural Biology, which is set to take place in the very 
accessible (and beautiful) Brussels, Belgium September 20-21st 2018.

We have put together a very exciting program covering many facets of structural 
biology.

Please take a look at:
https://vibconferences.be/event/structural-dynamics-in-cellular-communication-2nd-edition
 

 

The early-bird registration deadline is on August 10th.

On behalf of the organizers,
Savvas


---
Savvas Savvides
VIB Center for Inflammation Research
Dept. of Biochemistry & Microbiology, Ghent University
Technologiepark 927, B-9052 Ghent (Zwijnaarde), Belgium
+32 (0)472 928 519 (mobile) ; +32 9 331 36 60 (office) ; Skype: 
savvas.savvides_skype
http://www.vib.be/en/research/scientists/Pages/Savvas-Savvides-Lab.aspx 




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