[ccp4bb] 2 postdoctoral positions- structural biology of ubiquitin signaling

[Shaun Olsen]

Dear Colleagues,
 
My laboratory is seeking to fill two postdoctoral positions that are 
immediately available for highly motivated biochemists/structural biologists 
interested in studying molecular mechanisms of signaling by ubiquitin and 
ubiquitin-like proteins.  

Posttranslational modification of proteins by ubiquitin and ubiquitin-like 
modifiers occurs through the sequential activities of a cascade of enzymes and 
a major focus of the laboratory is to understand the structural basis by which 
this enzymatic machinery functions.  We address these issues using a 
multidisciplinary approach that combines X-ray crystallography, 
biochemical/biophysical techniques, and cell-based studies.  Candidates should 
hold a PhD in a relevant field with demonstrated expertise in X-ray 
crystallography and/or the biochemical analysis of protein ubiquitination.

MUSC is well-equipped for all aspects of X-ray crystallography, including 
institutional membership of the SER-CAT facility at the Advanced Photon Source. 
 We have a vibrant and growing program in structural biology and work in a 
highly collaborative environment. The University is located in historic 
Charleston, SC where the weather is beautiful year-round.  There are several 
beaches within minutes of the city and the mountains are only three hours away. 
 In addition to these amenities, the art, history, food, and festivals make 
Charleston a fantastic place to work and live.

Interested candidates should e-mail a cover letter, CV, and the names of three 
references to olse...@musc.edu and apply online at 
http://careers.pageuppeople.com/756/cw/en-us/job/510945/univ-postdoctoral-scholar-biochemistry-molecular-biology.

The positions will remain open until filled.
 
Best Regards,

-Shaun

-
Shaun K. Olsen, PhD
Assistant Professor
Dept. of Biochemistry & Molecular Biology
Medical University of South Carolina
Charleston, SC 29425
olse...@musc.edu

List of Publications-- 
https://www.ncbi.nlm.nih.gov/myncbi/browse/collection/48468424/?sort=date=descending



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[ccp4bb] Postdoc position to investigate the structural biology of ubiquitin ligases

[Sonja Lorenz]

Postdoc Position
Structural Mechanisms of Ubiquitin Ligases in Disease

We invite applications for a postdoc position in the research group of Dr. 
Sonja Lorenz at the Rudolf Virchow Center for Experimental Biomedicine, 
Würzburg, Germany. The Lorenz lab explores the structural mechanisms of 
ubiquitin ligases and their functions in tumorigenesis and infectious disease 
(see eLife 2017;6:e21036). We combine cryo-EM and X-ray crystallography with 
biophysical, biochemical, and cell biological analyses.

The Rudolf Virchow Center is a highly competitive, international research 
institute with outstanding infrastructure. The Lorenz lab has access to 
state-of-the-art instrumentation in cryo-EM, X-ray crystallography, and 
high-field NMR, and an extensive range of modern biophysical and cell 
biological equipment. We are integrated into a stimulating research environment 
that includes the Biocenter, the Research Center for Infectious Diseases, the 
University Hospital, the Max Planck Research Group in Systems Immunology, and 
the Helmholtz Institute for RNA-based Infection Research among others.

Würzburg has a particularly strong expertise in ubiquitin research, as 
exemplified by our DFG-funded Research Training Group “Understanding 
Ubiquitylation”: http://www.uni-wuerzburg.de/grk2243/home/ 


Moreover, the Lorenz lab is involved in a DFG-research unit “Therapeutic 
windows” and the Mildred-Scheel Center for Cancer Research, which is funded by 
the Deutsche Krebshilfe.
https://www.biozentrum.uni-wuerzburg.de/for2314/startseite/ 

https://www.biozentrum.uni-wuerzburg.de/msnz/startseite/ 


Since 2017, our lab also benefits from the EMBO Young Investigator Program.

Applicants should have strong expertise in protein X-ray crystallography, 
biochemistry, and biophysical techniques and a particular interest in cryo-EM.

English language skills are essential; knowledge of German is not required.
The position can start immediately or upon mutual agreement. Salary will be 
according to the German TVL scale. In case of equivalent qualifications, 
disabled applicants will be preferentially considered.
Würzburg is located in a beautiful, wine-growing area in proximity to Frankfurt 
(1 hour by train) and Munich (2 hours by train).

Applications should be sent to Sonja Lorenz by email and include a letter of 
motivation detailing research interests, expertise, and career aims; a CV; and 
the contact information for at least two references. Review of applications 
will begin immediately. The application deadline is 31 December 2018.

Lab homepages:
http://virchow.uni-wuerzburg.de/lab_pages/slorenz/ 

https://www.uni-wuerzburg.de/en/rvz/research/research-groups/lorenz-group/ 


For applications and questions, please contact Sonja Lorenz
E-mail: sonja.lor...@virchow.uni-wuerzburg.de 



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Re: [ccp4bb] Large scale insect cell expression

[radu]

Hi Joseph,

Apologies for not answering your question really :-) But, unless you plan to
focus on very peculiar insect cell proteins, I would suggest considering HEK
cells. See PMID: 30455477 for one of several possible systems. Cheaper,
easier, wave bag-friendly... Overall better than insect, especially for
membrane and secreted proteins. All vectors are with Addgene, early passage
cells from ATCC.

Best wishes,

Radu

-- 
Radu Aricescu
MRC Laboratory of Molecular Biology
Francis Crick Avenue
Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44-(0)1223-267049
fax: +44-(0)1223-268305
www: http://www2.mrc-lmb.cam.ac.uk/group-leaders/a-to-g/radu-aricescu

> Dear ccp4 community,
>
>
> I'll soon start my own lab and together with other colleagues, would like to
> establish a facility to do large scale insect cell culture for
> crystallography. Has anyone had any experience with the wave bag systems for
> this purpose (for instance from GE)? I have heard that culture infection can
> be a problem with this system.
>
>
> Any advice or other helpful tips in this regard would be much appreciated.
>
>
> Best regards,
>
> Joseph.
>
>
>
>
> Joseph S. Brock | PhD
> Researcher
> Drew Lab
> Department of Biochemistry and Biophysics
> Stockholm University
>
> 
>
> To unsubscribe from the CCP4BB list, click the following link:
> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
>



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[ccp4bb] Large scale insect cell expression

[Joseph Brock]

Dear ccp4 community,


I'll soon start my own lab and together with other colleagues, would like to 
establish a facility to do large scale insect cell culture for crystallography. 
Has anyone had any experience with the wave bag systems for this purpose (for 
instance from GE)? I have heard that culture infection can be a problem with 
this system.


Any advice or other helpful tips in this regard would be much appreciated.


Best regards,

Joseph.




Joseph S. Brock | PhD
Researcher
Drew Lab
Department of Biochemistry and Biophysics
Stockholm University



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Re: [ccp4bb] CCP4 Study Weekend: late addition and last chance to register - Registration extended to midnight Wednesday 12th December (tomorrow)!

[Karen McIntyre - UKRI STFC]

Due to problems with the website, we have extended registration to close at 
midnight tomorrow.

Regards
 
Karen McIntyre
CCP4 Study Weekend Local Organising Committee
Science & Technology Facilities Council
Scientific Computing Department - CCP4
RCaH 1.22
 
Tel +44 (0) 1235 44 5790
Fax  +44 (0) 1235 56 7720

. @ccp4_mx

STFC is part of UK Research and Innovation 
For more information visit https://stfc.ukri.org/
 



**Please note that I only work mornings until 1.30pm**

-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Randy Read
Sent: 07 December 2018 09:02
To: ccp4bb
Subject: [ccp4bb] CCP4 Study Weekend: late addition and last chance to register

The CCP4 Study Weekend on "Integrated, rational molecular replacement" will be 
held at the East Midlands Conferenced Centre in Nottingham, from 8th to 10th 
January 2019.  There are still some places left, but registration will close 
very soon, on 10th December.

We're pleased to announce a late addition to the programme, which couldn't be 
publicised until the results of the CASP13 protein folding challenge had been 
announced.  As you may have heard (if you read tech websites or the Guardian 
newspaper) the people from DeepMind have applied their deep learning algorithms 
to the protein folding problem and have done extremely well in folding the 
protein targets in CASP13.  Andrew Senior, the lead of the AlphaFold team that 
achieved this result, will be attending the Study Weekend to speak about their 
algorithms and their potential applications.

To see the rest of our exciting programme and to find registration information, 
please go to the meeting website: http://www.cvent.com/d/sgq8q6/6X.

Best wishes,

Isabel Usón, Ronan Keegan and Randy Read
--
Randy J. Read
Department of Haematology, University of Cambridge
Cambridge Institute for Medical Research  Tel: + 44 1223 336500
Wellcome Trust/MRC Building   Fax: + 44 1223 336827
Hills RoadE-mail: rj...@cam.ac.uk
Cambridge CB2 0XY, U.K.   www-structmed.cimr.cam.ac.uk



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