[ccp4bb] Postdoctoral Positions in Engineering Ion Channels and Transporters

[Francis Valiyaveetil]

PostdoctoralPositions in Engineering Ion channels and Transporters
  Two postdoctoral positions are availablein the Valiyaveetil group at 
Oregon Health and Science University in Portland,Oregon. The Valiyaveetil group 
investigates structure and function of ionchannels and transporters using a 
combination of unnatural amino acid mutagenesis,electrophysiology, structural 
biology and spectroscopy.  See references [Nat Commun, (2019), 9, 5055; PNAS 
(2019) 116,15939-15946] forexamples of recent research from the lab. The group 
is a part of the Program in Chemical Biology in the Departmentof Chemical 
Physiology and Biochemistry.

  The positions are suitable for highlymotivated candidates with a 
recent (less than 1 year) Ph.D. in Biochemistry, Biophysicsor a related 
discipline.  The first candidatewill investigate ion channels using unnatural 
amino acid mutagenesis, functionaland structural studies.  Candidates forthis 
position should have prior research experience in ion channels and have 
expertisein carrying out electrophysiological measurements using two-electrode 
voltage clampor patch clamp.  The second candidatewill investigate transporters 
using a combination of biochemical, spectroscopicand structural approaches.  
Candidatesfor this position should have prior experience in membrane protein 
biochemistryand prior expertise in structural approaches such as 
crystallography or cryo-EMor in fluorescence or EPR spectroscopy will be a 
plus.  These positions provides the candidates witha unique opportunity to 
enhance their skill set with the techniques for membraneprotein engineering 
using unnatural amino acid mutagenesis.  

  The Valiyaveetil lab is well equippedto carry out structural and 
functional studies of ion channels and transporters.  OHSU provides an 
excellent scientificenvironment.  Portland is consistentlyrated as one of the 
most livable cities in the US.  

  To apply send CV, a 1-2 pagedescription of graduate research and 
future research plans along with contactinformation for three references by 
email to: valiy...@ohsu.edu  





To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1

Re: [ccp4bb] E. coli BirA biotin ligase expression/purification

[radu]

Dear Gloria,

I think that the most economical biotinylation manner is in vivo, by
co-expression of a tagged protein with BirA. One can PCR amplify the BirA cDNA
from E. coli and clone it into plasmids for any expression system and
sub-cellular localization needed. Ready to use BirA expression plasmids are
also available on Addgene. Some extra biotin has to be added to culture medium
in my experience. Examples from our lab (HEK cells) are in PMID: 27418511 and
28817804.

Best wishes,

Radu


> Dear friends in crystallography,
> I know this seems unrelated, but it really isn't ... please forgive me. We
are trying to use the Avitag/BirA system to specifically biotinylate target
proteins in an economical manner.  Are any of you purifying the BirA enzyme
in your lab for biotinylation and if so can you help me figure out the right
plasmid/purification protocol?   Please let me know.  Thank you. Gloria
>  To
unsubscribe from the CCP4BB list, click the following link:
> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1


-- 
Radu Aricescu
MRC Laboratory of Molecular Biology
Francis Crick Avenue
Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44-(0)1223-267049
fax: +44-(0)1223-268305
www: http://www2.mrc-lmb.cam.ac.uk/group-leaders/a-to-g/radu-aricescu



To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1

Re: [ccp4bb] E. coli BirA biotin ligase expression/purification

[Olson, Linda]

Dear Gloria,

This is the basic protocol we follow.  Hope it helps

Arnaud Gautier and Marlon J. Hinner (eds.), Site-Specifi c Protein Labeling: 
Methods and Protocols, Methods in Molecular Biology,
vol. 1266, DOI 10.1007/978-1-4939-2272-7_12, © Springer Science+Business Media 
New York 2015

Linda



From: CCP4 bulletin board  on behalf of Gloria Borgstahl 

Sent: Monday, October 21, 2019 12:22 PM
To: CCP4BB@JISCMAIL.AC.UK 
Subject: [ccp4bb] E. coli BirA biotin ligase expression/purification

ATTENTION: This email originated from a sender outside of MCW. Use caution when 
clicking on links or opening attachments.

Dear friends in crystallography,
I know this seems unrelated, but it really isn't ... please forgive me.
We are trying to use the Avitag/BirA system to specifically biotinylate target 
proteins in an economical manner.  Are any of you purifying the BirA enzyme in 
your lab for biotinylation and if so can you help me figure out the right 
plasmid/purification protocol?   Please let me know.  Thank you.  Gloria



To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1



To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1

[ccp4bb] E. coli BirA biotin ligase expression/purification

[Gloria Borgstahl]

Dear friends in crystallography,
I know this seems unrelated, but it really isn't ... please forgive me.
We are trying to use the Avitag/BirA system to specifically biotinylate
target proteins in an economical manner.  Are any of you purifying the BirA
enzyme in your lab for biotinylation and if so can you help me figure out
the right plasmid/purification protocol?   Please let me know.  Thank you.
Gloria



To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1

Re: [ccp4bb] off topic: microscope in glove box

[Artem Evdokimov]

Short version: It should be OK, especially since the vacuum is transient
and not particularly 'strong*' :)

Long version: if this is an older microscope there may be further
delamination of optically bonded components if air is already admitted
between glass planes (i.e. the optical cement is worn and old). Also some
of the fancier models may have pneumatic balance elements for gross motion
of the optical column - those may experience pressure differentials above
their maximum tolerances. Finally, and very unlikely you may have a
situation where multiple optical elements are sealed together in a single
tube with air trapped between them - if the 'vent' is blocked (by e.g. old
grease or something) then these may pop.

https://www.olympus-lifescience.com/en/microscope-resource/primer/anatomy/oculars/


But the short version is right 99% of the time.

Artem

*"Professor Hubert Farnsworth
: Well, it's a space ship,
so I'd say anywhere between zero and one."
https://www.imdb.com/title/tt0584455/characters/nm0921942

- Cosmic Cats approve of this message


On Mon, Oct 21, 2019 at 10:00 AM Guenter Fritz <
guenter.fritz.phenix.c...@gmail.com> wrote:

> Dear all,
>
> I want to put one of our microscopes into the glove box. Does anybody
> know whether some parts of the microscope optics do not like vacuum in
> the air lock ?
>
> Thanks and best regards, Guenter
>
> 
>
> To unsubscribe from the CCP4BB list, click the following link:
> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
>



To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1

[ccp4bb] off topic: microscope in glove box

[Guenter Fritz]

Dear all,

I want to put one of our microscopes into the glove box. Does anybody 
know whether some parts of the microscope optics do not like vacuum in 
the air lock ?


Thanks and best regards, Guenter



To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1

[ccp4bb] PhD position in Structural Biology - Biozentrum, University of Basel; Switzerland

[Urs Jenal]

PhD student, 100%, Structure-function analysis of toxin-antitoxin systems in 
the human pathogen Pseudomonas aeruginosa.
The Biozentrum of the University of Basel is one of the world’s leading Life 
Sciences Institutes with over 30 research groups and 500 employees that 
research how molecules and cells create life, spanning the scale from atoms to 
organisms. Founded in 1971, the Biozentrum has been the birth place of many 
fundamental discoveries in biology and medicine, spawning several Nobel 
Laureates.
A PhD position is available at the Biozentrum within an SNSF-funded project on 
«The role of toxin/antitoxin modules in Pseudomonas aeruginosa phenotypic 
heterogeneity and antibiotic tolerance». The project aims at reaching a 
molecular and cellular understanding of phenotypic heterogeneity in populations 
of P. aeruginosa and how this contributes to virulence and persistence of this 
important human pathogen.
We are seeking excellent and highly motivated candidates with interest and 
training in structural biology and biochemistry. Successful applicants will be 
working in an interdisciplinary team of researchers investigating mechanisms of 
virulence and persistence of the human pathogen P. aeruginosa. The team 
includes experts in molecular microbiology, computational biology and 
structural biology. The new team member will be responsible for the biochemical 
and structural analysis of toxins and will probe their interaction with the 
respective antitoxins.
We offer an outstanding and highly interactive scientific environment, 
state-of-the-art technology platforms, competitive salaries, and opportunities 
for advanced training. The new team member will be joining the international 
Biozentrum PhD program, a community of over 130 young scientists from around 
the world, with a strong mentoring program and access to a post-graduate 
teaching program. The position is available immediately and is funded for four 
years.
Basel is a cosmopolitan and multicultural city at the heart of Europe. 
Bordering three countries, Switzerland, Germany, and France, Basel provides a 
high standard of living with a thriving cultural atmosphere. The Basel area is 
Europe’s most important Life Science hub with many small and medium-size 
biotech companies as well as global pharmaceutical players.
Please send your application (cover letter, CV, diplomas and contact 
information of two referees) to Prof. Urs Jenal, Biozentrum, University of 
Basel, Klingelbergstrasse 70, 4056 Basel, Switzerland, e-mail: 
urs.je...@unibas.ch. For further information, 
please see 
www.biozentrum.unibas.ch/jenal or 
contact urs.je...@unibas.ch.





















To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1

[ccp4bb] PhD and postdoc position – Humboldt-Universität Berlin Germany

[Holger Dobbek]

Dear colleagues,

We are looking for a PhD candidate (m/f/d) and a Postdoctoral researcher 
(m/f/d) to strengthen our structural biology/biochemistry team at the 
Humboldt-Universität zu Berlin (HU).

Our research focuses on the biochemical principles how archaea and bacteria use 
carbon monoxide (CO) and carbon dioxide (CO2) as carbon and/or energy source. 
We use protein crystallography in combination with protein 
chemistry/spectroscopy and kinetics to study the structure, mechanism and 
assembly of the (multi)complex metalloenzymes at the heart of these molecular 
energy conversions.

Our laboratory has state-of-the-art facilities for protein production, 
biochemical characterisation, and protein crystallisation/x-ray 
crystallography. HU is a member of the Joint Berlin MX-Laboratory, enabling 
direct access to the local synchrotron facility (BESSY II, Berlin).

For more details see: 
https://www.biologie.hu-berlin.de/en/gruppenseiten-en/struktbio 


Postdoctoral position:
You should have a university degree and PhD in biochemistry, chemistry, 
biophysics or biology. Expertise in either x-ray crystallography, preparative 
(recombinant) protein production and/or functional characterisation is 
essential. Previous research experience should be documented through 
peer-reviewed publications (at least one as first author).
The focus of your contribution to the research projects will be on 
cofactor-dependent enzymatic reactions, protein crystallography and enzymology. 
You will contribute to the lectures by assisting in the design, organisation 
and supervision of lectures, seminars and practical courses. These tasks will 
allow you to acquire and train skills for further academic qualifications.

PhD position:
You should have a university degree in biochemistry, chemistry, biophysics or 
biology. Experience in biochemical, biophysical and structural biology methods, 
especially in the characterisation of enzymes is welcome.
You will work in the research project “Fe/S-double-cubane-cluster: a new 
cofactor in biology (DCCP)” within the priority program “Fe/S-clusters for 
life” (https://www.ironsulfurforlife.de ). 
Your tasks are the purification and the biochemical and structural 
characterization of oxygen-sensitive metalloenzymes in order to elucidate the 
role of Fe/S-double cubane-clusters.

Both positions are funded for 3 years (German salary level E 13 TV-L HU for 
postdoctoral fellow, 0,65 E 13 TV-L for PhD fellow). Please submit your 
application (letter of application, CV and two references) preferentially 
electronically (a single PDF file not exceeding 10 MB) with a subject line 
“AN/300/19” for the postdoctoral position and “DR/187/19” for the PhD position. 
Deadline for application is 30.10.2019.

Email contact: holger.dob...@biologie.hu-berlin.de 


Holger Dobbek
Structural Biology/Biochemistry
Humboldt-Universität zu Berlin
Germany




To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1

Re: [ccp4bb] CCP4 Computational Scientist

[David Waterman]

Dear all,

Here's a reminder that CCP4 is looking for a computational scientist to
investigate corrections for electron diffraction data. Job details are
below.

Best wishes

-- David


On Mon, 23 Sep 2019 at 13:20, Eugene Krissinel 
wrote:

> Dear All,
>
> CCP4 is looking for computational scientist to work on a research project
> in Electron Diffraction. The Project is funded by BBSRC UK, and will be
> conducted in CCP4 Core Group based in Rutherford Appleton Laboratory, UK.
>
> For full job details and direct links to online application portal, please
> visit one of the following web-sites:
>
> https://www.topcareer.jobs/Vacancy/irc250548_10011.aspx
> https://findajob.dwp.gov.uk/details/3026333
> https://brightrecruits.com/jobs/ccp4-computational-scientist
>
> All informal enquires may be sent to eugene.krissi...@stfc.ac.uk
>
> Kind regards,
> Eugene Krissinel.
>
>
>
>
>
>
> --
>
> To unsubscribe from the CCP4BB list, click the following link:
> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
>



To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1