Re: [ccp4bb] Co crystalization with less soluble ligand.
Hi Frank, heme becomes near completely insoluble when it dimerizes. Fatty acids are also very insoluble. It is quite hard to get them into HSA, as far as I remember. Cheers, Tim On Wed, 23 Jun 2021 10:33:42 +0100 Frank von Delft wrote: > And then of course, you need to decide whether you at all care to > know anything about a compound that is so insoluble that it needs > that kind of treatment :) > > On 23/06/2021 09:52, hoh wrote: > > Hi > > > > As total insolubility does not exist, I regularly use another > > method, which is to deposit a grain of the ligand directly into the > > drop. > > > > In this case, we no longer control the concentration. The goal is > > to regularly freeze crystals (1 hour, 2 days, 1 week ..). Depending > > on the results, it is possible to adjust the time. If after one > > hour, the crystal no longer diffracts, redo the experience by > > freezing at 10s, 30s, 2mn ..). If a blob appears, frozen after 1 > > week. This technique needs 2 things > > , time and several exploitable crystals in the drop. > > > > > > FH > > > > > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 > > This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a > mailing list hosted by www.jiscmail.ac.uk, terms & conditions are > available at https://www.jiscmail.ac.uk/policyandsecurity/ -- -- Tim Gruene Head of the Centre for X-ray Structure Analysis Faculty of Chemistry University of Vienna Phone: +43-1-4277-70202 GPG Key ID = A46BEE1A To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/ pgpiKZin1MEqF.pgp Description: OpenPGP digital signature
Re: [ccp4bb] Co crystalization with less soluble ligand.
And then of course, you need to decide whether you at all care to know anything about a compound that is so insoluble that it needs that kind of treatment :) On 23/06/2021 09:52, hoh wrote: Hi As total insolubility does not exist, I regularly use another method, which is to deposit a grain of the ligand directly into the drop. In this case, we no longer control the concentration. The goal is to regularly freeze crystals (1 hour, 2 days, 1 week ..). Depending on the results, it is possible to adjust the time. If after one hour, the crystal no longer diffracts, redo the experience by freezing at 10s, 30s, 2mn ..). If a blob appears, frozen after 1 week. This technique needs 2 things , time and several exploitable crystals in the drop. FH To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
[ccp4bb] Co crystalization with less soluble ligand.
Hi As total insolubility does not exist, I regularly use another method, which is to deposit a grain of the ligand directly into the drop. In this case, we no longer control the concentration. The goal is to regularly freeze crystals (1 hour, 2 days, 1 week ..). Depending on the results, it is possible to adjust the time. If after one hour, the crystal no longer diffracts, redo the experience by freezing at 10s, 30s, 2mn ..). If a blob appears, frozen after 1 week. This technique needs 2 things , time and several exploitable crystals in the drop. FH -- François Hoh Centre de Biochimie Structurale, UMR 5048 CNRS, UMR 1054 INSERM 29, rue de navacelles 34090 Montpellier Cedex, France. Phone: +33 467 417 706 Fax: +33 467 417 913 -- This message has been scanned for viruses and dangerous content by MailScanner, and is believed to be clean. To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
