Re: [ccp4bb] Any suggestions?

[Dr. Isabel De Moraes]

Dear all,

My apologies for not being able to see the pictures. I have attached them as 
PDFs here but not sure if you will be able to get the files.

Anyway, many thanks to all who have reply via ccp4bb or privately with 
suggestions.  Indeed the protein is purified from the natural source so there 
are numerous possibilities.

Kind regards,
Isabel







On 7 Jul 2017, at 12:57, Dr. Isabel De Moraes 
<isabel.de-mor...@diamond.ac.uk<mailto:isabel.de-mor...@diamond.ac.uk>> wrote:

Any suggestions regarding to the positive densities?

The crystallisation condition only has NaCl and CaCl



[cid:DD08D41F-CAB5-458D-BBD0-5DDCADC746FF@diamond.ac.uk]




[cid:B6BDEE5E-325C-4FEF-A7BC-B4F679D5315F@diamond.ac.uk]


Best regards,
Isabel




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-
Dr Isabel Moraes

Membrane Protein Laboratory  Group Leader

Diamond Light Source Ltd,
Harwell Science and Innovation Campus,
Oxfordshire, OX11 ODE, UK
-

[cid:b2df3ed8-aa8e-4a06-a691-8732bdbf96ce@fed.cclrc.ac.uk]

[ccp4bb] Any suggestions?

[Dr. Isabel De Moraes]

Any suggestions regarding to the positive densities?

The crystallisation condition only has NaCl and CaCl



[cid:DD08D41F-CAB5-458D-BBD0-5DDCADC746FF@diamond.ac.uk]




[cid:B6BDEE5E-325C-4FEF-A7BC-B4F679D5315F@diamond.ac.uk]


Best regards,
Isabel




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[ccp4bb] Research Technician for the Membrane Protein Laboratory (MPL) at Diamond Light Source and RCAH in Oxfordshire

[Dr. Isabel De Moraes]

Dear all,



Applications are invited for a full-time Research Technician position at the 
Membrane Protein Laboratory (MPL) located at the Diamond Light Source and at 
the Research Complex (Oxfordshire).

The MPL is Wellcome Trust funded facility created to allow scientists to 
determine medically relevant membrane protein structures more efficiently by 
combining recently developed high throughput technologies for protein 
production, crystallization, XFEL and EM. The MPL is located in laboratories 
around the ring at Diamond and also at the Research Complex at Harwell and 
equipped with state-of-the-art equipment.

The successful applicant will report directly to Dr Isabel Moraes, the MPL 
Group Leader. In the group he/she would assist in all aspects of the lab work, 
in particular repair and maintenance of crystallisation robots, assisting with 
training users in crystallisation with robots; production and crystallisation 
of membrane proteins. The technician will also be responsible for the general 
running of the laboratory, including placing orders, maintaining stocks of 
chemicals, preparing solutions, maintaining all the equipment and general 
laboratory maintenance. The applicant will also have a role in maintaining 
safety standards and documentation and have excellent communication skills.

For further details  about the position and how to apply please visit   
http://www.diamond.ac.uk/Careers/Vacancies/All/028_17_CH.html


Informal enquiries may be made to Dr Isabel Moraes, the MPL group leader 
(isabel.de-mor...@diamond.ac.uk)


Best regards,
Isabel Moraes

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Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom

[ccp4bb] Research Assistant for the Membrane Protein Laboratory (MPL) at Diamond Light Source and RCAH in Oxfordshire

[Dr. Isabel De Moraes]

Dear all,


Applications are invited for a full-time Research Assistant position at the 
Membrane Protein Laboratory (MPL) located at the Diamond Light Source and at 
the Research Complex (Oxfordshire).

The MPL is Wellcome Trust funded facility created to allow scientists to 
determine medically relevant membrane protein structures more efficiently by 
combining recently developed high throughput technologies for protein 
production, crystallization, XFEL and EM. The MPL is located in laboratories 
around the ring at Diamond and also at the Research Complex at Harwell and 
equipped with state-of-the-art equipment.

The successful applicant will report directly to Dr Isabel Moraes, the MPL 
Group Leader. In the group he/she will primarily assist the MPL users with 
molecular biology (cloning), expression in insect cell and mammalian, protein 
purification and/or crystallisation. In addition, the post holder should be 
familiar with all the facility equipment and also be involved in the in the 
day-to-day running of the laboratory, including equipment maintenance, ordering 
and health and safety procedures (Risk Assessments, GM forms and COOSH). The 
person will also be involved in sample preparation for EM and XFEL applications.

The successful candidate should have a Masters degree in biological sciences, 
or the equivalent in professional qualifications. In addition to good English 
verbal and written communication skills, methodical approaches to work and the 
ability to pay close attention to details is also desired.  You will also have 
good interpersonal and organisational skills as well as the ability to work as 
part of a team.

For further details  about the position and how to apply please visit   
http://www.diamond.ac.uk/Careers/Vacancies/All/027_17_CH.html


Informal enquiries may be made to Dr Isabel Moraes, the MPL group leader 
(isabel.de-mor...@diamond.ac.uk)


Best regards,
Isabel Moraes



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Re: [ccp4bb] Dimer in SDS-PAGE

[Dr. Isabel De Moraes]

Dear Amit,

You are working with a membrane protein and the use of SDS (harsh detergent)  
often makes these proteins to oligomerise.  Boiling your sample is not 
advisable, it might make it worse.

I’m not sure why you would like to “see” a monomer in the gel but if you really 
would like to know what is the oligomeric state of you protein in solution, I 
would advise you to do a SEC-MALS (size exclusion chromatography - multi angle 
light scattering) run of your sample.

Best,
Isabel


-
Dr Isabel Moraes

Membrane Protein Laboratory  Group Leader

Diamond Light Source Ltd,
Harwell Science and Innovation Campus,
Oxfordshire, OX11 ODE, UK
-


On 21 Feb 2017, at 22:22, amit gaur 
> wrote:

Hi all,
  I am trying to purify a potassium ion channel from insect cell using 
baculovirus expression system. I am not seeing monomer of this protein in SDS 
instead a dimer appears.So,I increased DTT in SDS buffer but no change and 
dimer was intact. In size exclusion this protein appeared as a tetramer which 
is common oligomerizaton of potassium channel family with GYG motif. Can any 
body suggest what should I do in this case?

Thanks and regards,


--
Dr. Amit Gaur
Post Doctoral Researcher
PI: Dr. Ji-Fang Zhang
Thomas Jefferson University
1020, Locust Street, Suite 418
Philadelphia, PA 19107





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[ccp4bb] FREE Symposium on Membrane Protein Sample Preparation & Analysis

[Dr. Isabel De Moraes]

Dear CCP4 colleagues,



The Membrane Protein Laboratory (MPL) at the Diamond Light Source and GE 
Healthcare are organising a FREE Symposium on Membrane Protein Sample 
Preparation & Analysis.

 The symposium will take place on the 10th November, 9:30am at the Pickvance 
Lecture Theatre, Harwell Campus, Oxfordshire.

 Join us for a day of membrane protein discussion, technological insights and 
success stories.



Speakers include:

Prof. Roslyn Bill – Aston University

Dr Nicola Burgess-Brown – SGC Oxford

Prof Tim Dafforn – University of Birmingham

Dr Roger Dodd – University of Cambridge/Medimmune (TBC)

Dr James Errey – Heptares

Dr Iva Hopkins Navratilova - University of Glasgow

Prof Chris Tate – MRC LMB

Prof Bonnie Wallace - Birkbeck College

Prof Tony Watts – University of Oxford


For registration: 
https://supportcentral.ge.com/esurvey/takesurvey.asp?p=17778=3986083

Best regards,
Isabel Moraes



-
Dr Isabel Moraes

Membrane Protein Laboratory  Group Leader

Diamond Light Source Ltd,
Harwell Science and Innovation Campus,
Oxfordshire, OX11 ODE, UK
-


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Re: [ccp4bb] TM purification & crystallization

[Dr. Isabel De Moraes]

Dear Andre,

It is a small protein then, I recommend to do the purification in a small 
micelle detergent (e.g. OG). This regardless the  extraction done in DDM or DM 
or any other suitable detergent.

You can start by reading

Moraes, Isabel, et al. "Membrane protein structure determination—the next 
generation." Biochimica et Biophysica Acta (BBA)-Biomembranes 1838.1 (2014): 
78-87
(http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3898769/)

or

Moraes, Isabel & Margarida Archer. "Methods for the Successful Crystallization 
of Membrane Proteins." Structural Proteomics: High-Throughput Methods (2015): 
211-230


Best regards,
Isabel


Dr Isabel Moraes

Head of the Membrane Protein Laboratory

Diamond Light Source Ltd,
Harwell Science and Innovation Campus,
Oxfordshire, OX11 ODE, UK




On 28 Jul 2016, at 12:47, Andre Godoy 
<0b9d7671a1b6-dmarc-requ...@jiscmail.ac.uk>
 wrote:

Dear community

we are starting the study of a 20 kDa alpha transmembrane domain, and we were 
wondering if one could recommend any particular detergent (and its 
concentration) that won't influence the concentration step for crystallization. 
Tips for crystallization using detergents and LCP would also be helpful.

any comment would be appreciated

All the best,


Andre Godoy



Dr Isabel Moraes

Head of the Membrane Protein Laboratory

Diamond Light Source Ltd,
Harwell Science and Innovation Campus,
Oxfordshire, OX11 ODE, UK

http://www.diamond.ac.uk/Beamlines/Mx/MPL.html







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[ccp4bb] Position for a Research Technician at the Membrane Protein Laboratory at Diamond Light Source

[Dr. Isabel De Moraes]

To all who might be interested

We are seeking a full-time Research Technician to work at the Membrane Protein 
Laboratory (MPL) located at the Diamond Light Source in Oxfordshire.
The MPL at Diamond Light Source is a Wellcome Trust funded facility created in 
collaboration between Imperial College London and Diamond Light Source. This 
laboratory allows scientists to determine medically relevant membrane protein 
structures more efficiently by combining recently developed high throughput 
technologies for protein production/crystallisation and X-ray diffraction data 
collection system at Diamond.  The MPL is located in laboratories around the 
ring at Diamond and also at the Research Complex at Harwell.  MPL is equipped 
with state-of-the-art technology and equipment.
 The successful candidate will report directly to Dr Isabel Moraes, the MPL 
Group Leader.  In the group he/she would assist in all aspects of the lab work, 
in particular repairing and maintenance of crystallisation robots, assisting 
with training users in crystallisation with robots; production and 
crystallisation of membrane proteins. The technician will also be responsible 
for the general running of the laboratory, including placing orders, 
maintaining stocks of chemicals, preparing solutions, maintaining all the 
equipment and general laboratory maintenance. The applicant will also have a 
role in maintaining safety standards and documentation and have excellent 
communication skills.
 The successful candidate must have 2 A-Levels (or equivalent) in relevant 
subjects and experience in a technical or scientific role. A BSc in 
Biochemistry/Biology or equivalent is desirable. Practical experience with 
protein purification and laboratory-based research is essential while 
experience in Protein expression, Cell Culture and mutagenesis and cloning 
techniques is desirable. You also  must have excellent verbal and written 
communication skills, a methodical approach to work and the ability to pay 
close attention to details.  You will also have the ability to work with 
limited supervision, good interpersonal and organisational skills as well as 
the ability to work as part of a team.

These are full-time post available for up to 12 months in the first instance, 
with possibility of extension subject to funding.Informal enquiries may be made 
to Dr Isabel Moraes, the MPL group leader 
(i.mor...@imperial.ac.ukmailto:i.mor...@imperial.ac.uk). 
http://www.diamond.ac.uk/Beamlines/Mx/MPL.html

Closing date: 8 July 2014 (midnight BST)
To apply,  Job Description and Person Specification please refer to the 
following webpage
 
https://www4.ad.ic.ac.uk/OA_HTML/OA.jsp?page=/oracle/apps/irc/candidateSelfService/webui/VisVacDispPGakRegionApplicationId=821transactionid=1538103408retainAM=YaddBreadCrumb=Sp_svid=43842p_spid=1669138oapc=9oas=dHtHWqciP0_3C_VtnV-LXQ



Kind regards,
Isabel

Dr Isabel De Moraes, MRSC

Membrane Protein Laboratory
Diamond Light Source



Dr Isabel De Moraes, MRSC

Head of the Membrane Protein Laboratory

Diamond Light Source Ltd,
Harwell Science and Innovation Campus,
Chilton, Didcot, Oxfordshire,
OX11 ODE, UK







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Re: [ccp4bb] Royal Institution Lecture

[Dr. Isabel De Moraes]

Dear Stephen, 

Many thanks for this post.  

The lecture is really great, I would recommended it to all interested in the 
field (from beginners to seniors).

Best,
Isabel



Dr Isabel De Moraes, MRSC
 
Membrane Protein Laboratory
Diamond Light Source Ltd,
Harwell Science and Innovation Campus,
Chilton, Didcot, Oxfordshire,
OX11 ODE, UK 




On 3 Nov 2013, at 10:12, Curry, Stephen wrote:

 I hesitated to post this because crystallography is meat and drink to people 
 around here, but in case it might be useful for teaching, here is the video 
 of a lecture I gave on X-ray crystallography at the Royal Institution on 
 Friday 25th October. Warning: it lasts a full hour!
 
 http://www.richannel.org/seeing-things-in-a-different-light
 
 Stephen 




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Re: [ccp4bb] membrane protein optimization

[Dr. Isabel De Moraes]

Dear  Frank,

It is difficult to say something without the diffraction images but  probably 
they are detergent ( or lipid) crystals.  These crystals are in general soft 
and don't diffract well.

My suggestion is to know well what your crystals really are before any 
optimization.

Best,
Isabel

Dr Isabel De Moraes, MRSC

Membrane Protein Laboratory
Diamond Light Source Ltd,
Harwell Science and Innovation Campus,
Chilton, Didcot, Oxfordshire,
OX11 ODE, UK




On 23 Oct 2013, at 17:22, crystalboy wrote:

 Hi CCP4BB Forks,

 In recently I got a membrane protein crystal in the quite normal
 membrane protein crystallization conditions as other persons reported,
 like PEG400 16-19%, pH 6.0-7.5, with 50 mM MgCl2 (in my case) by using
 sitting drop method. These crystals are around 50-100 uM. They look
 like trapezoid crystal. My problem is all of my crystals have not
 diffraction in home source X-ray and just poor diffraction at
 Synchrotron (lower than 20 A). My crystals like to appear on the
 surface of the drop. Look like my crystals are quite light.  I had
 tried to use a needle to touch them. Unlike other protein crystal, my
 crystal looks like quite soft. When I touch it, it didn't crack, but
 was bend or mashed.  I had tried to do additive screen and detergent
 screen. It seems they are not useful.

 Do anyone have good ideas to optimization these crystals? Thanks for
 your suggestions.

 Frank
[cid:7839c9ff-b567-4b4b-b191-07dc6c335f34@fed.cclrc.ac.uk]




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Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom

 

[ccp4bb] PhD position at the Membrane Protein Laboratory (Diamond)

[Dr. Isabel De Moraes]

Applications are invited for a 3-year PhD position at Imperial College London 
which will be based in The Membrane Protein Laboratory (MPL) at Diamond Light 
Source, Didcot, Oxfordshire and the Centre for Structural Biology, Imperial 
College London. The successful applicant, for the position which is immediately 
available, will work on structural studies of membrane transport proteins using 
microfocus X-rays and X-ray Free Electron Laser beams.  The project “NanoMem” 
is supported by the Marie Curie Initial Training Network (European Commission 
Framework Seven Programme).
The MPL operates both as a facility for users and as a cutting edge research 
laboratory. It is a high-throughput protein crystallisation facility that aims 
to enhance the rate of success in the crystallisation of medically significant 
membrane proteins. Its location at Diamond Light Source facilitates 
high-throughput crystal screening and high quality data collection.

The student will be co-supervised by Prof. So Iwata, Dr Isabel Moraes and Prof. 
Xiaodong Zhang at Imperial College. The student will be trained in 
state-of-the-art methods of membrane protein expression, purification, 
crystallization and structure determination using both synchrotron microfocus 
X-Ray beamlines and X-Ray free electron lasers.  The successful candidate, as a 
member of the Marie Curie Initial Training Network, will participate in 
collaborative experiments, secondments and training events across the network 
(The NanoMem network compromises ten laboratories from five European countries).

Studentship Details
Applicants should hold a Masters degree in Biochemistry or a related subject.
To apply: applications should include a cover letter describing relevant 
research experience to date, a CV, and the names and addresses of two referees.
These should be sent to Dr Isabel Moraes 
(i.mor...@imperial.ac.ukmailto:i.mor...@imperial.ac.uk) by email by 31th May 
2013.
This studentship is only available to applicants who are not UK citizens or 
have not lived in the UK for more than 12 months during the last three years.
For informal enquires please contact Dr Isabel Moraes  
(i.mor...@imperial.ac.ukmailto:i.mor...@imperial.ac.uk)




Dr Isabel De Moraes, MRSC

Head of the Membrane Protein Laboratory

Diamond Light Source Ltd,
Harwell Science and Innovation Campus,
Chilton, Didcot, Oxfordshire,
OX11 ODE, UK






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Diamond Light Source Limited (company no. 4375679). Registered in England and 
Wales with its registered office at Diamond House, Harwell Science and 
Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom

 

[ccp4bb] A FREE course in High-throughput Protein Production and Crystallisation

[Dr. Isabel De Moraes]

Dear All, 

A gentle reminder that the deadline for the  practical course in 
High-troughtput Protein Production and Crystallisation  is 20th January. 

For more details, please see below.

http://www.oppf.ox.ac.uk/OPPF/public/courses/oppfmpl2011.jsp

With best Regards,
Isabel




On 2 Dec 2010, at 15:52, Dr. Isabel De Moraes wrote:

 
 Dear Structural Biologists,
 
 The Oxford Protein Production Facility (OPPF-UK) and The Membrane Protein 
 Laboratory (MPL) at Diamond are offering a
 hands-on course in  High-throughput Protein Production and 
 Crystallization. 
 
 The course runs from 30th March to 7th April 2011 at and its aim is the 
 review of the state-of-the-art in HTP structural biology
 with an emphasis on methods to study complex and 'difficult' targets, 
 including membrane proteins.
 
 The course is FREE for participants from UK Academic Institutions. See flyer 
 attached.
 
 For more information please look into the following link: 
 http://www.oppf.ox.ac.uk/OPPF/public/courses/oppfmpl2011.jsp
 
 Deadline for applications is: 20th Janaury 2011.
 
 With Best Regards,
 Isabel Moraes
 
 
 
 
 Dr. Isabel De Moraes, MRSC
 
 Membrane Protein Laboratory Facilities Coordinator/Group Leader
 Membrane Protein Laboratory,
 Diamond Light Source Ltd,
 Harwell Science and Innovation Campus,
 Chilton, Didcot, Oxfordshire,
 OX11 ODE, UK
 
 Tel: 01235 778664
 email: isabel.de-mor...@diamond.ac.uk
 ---
 
 
 OPPF-MPL course poster.pdf




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Diamond Light Source Limited (company no. 4375679). Registered in England and 
Wales with its registered office at Diamond House, Harwell Science and 
Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom

 

Re: [ccp4bb] A FREE course in High-throughput Protein Production and Crystallisation

[Dr. Isabel De Moraes]

Dear All,

I would like to retract this post since the information is wrong.

My sincerely apologies. 

I will send the right information shortly.


Isabel





On 9 Jan 2013, at 11:16, Dr. Isabel De Moraes wrote:

 Dear All, 
 
 A gentle reminder that the deadline for the  practical course in 
 High-troughtput Protein Production and Crystallisation  is 20th January. 
 
 For more details, please see below.
 
 http://www.oppf.ox.ac.uk/OPPF/public/courses/oppfmpl2011.jsp
 
 With best Regards,
 Isabel
 
 
 
 
 On 2 Dec 2010, at 15:52, Dr. Isabel De Moraes wrote:
 
 
 Dear Structural Biologists,
 
 The Oxford Protein Production Facility (OPPF-UK) and The Membrane Protein 
 Laboratory (MPL) at Diamond are offering a
 hands-on course in  High-throughput Protein Production and 
 Crystallization. 
 
 The course runs from 30th March to 7th April 2011 at and its aim is the 
 review of the state-of-the-art in HTP structural biology
 with an emphasis on methods to study complex and 'difficult' targets, 
 including membrane proteins.
 
 The course is FREE for participants from UK Academic Institutions. See flyer 
 attached.
 
 For more information please look into the following link: 
 http://www.oppf.ox.ac.uk/OPPF/public/courses/oppfmpl2011.jsp
 
 Deadline for applications is: 20th Janaury 2011.
 
 With Best Regards,
 Isabel Moraes
 
 
 
 
 Dr. Isabel De Moraes, MRSC
 
 Membrane Protein Laboratory Facilities Coordinator/Group Leader
 Membrane Protein Laboratory,
 Diamond Light Source Ltd,
 Harwell Science and Innovation Campus,
 Chilton, Didcot, Oxfordshire,
 OX11 ODE, UK
 
 Tel: 01235 778664
 email: isabel.de-mor...@diamond.ac.uk
 ---
 
 
 OPPF-MPL course poster.pdf
 
 
 
 
 -- 
 
 This e-mail and any attachments may contain confidential, copyright and or 
 privileged material, and are for the use of the intended addressee only. If 
 you are not the intended addressee or an authorised recipient of the 
 addressee please notify us of receipt by returning the e-mail and do not use, 
 copy, retain, distribute or disclose the information in or attached to the 
 e-mail.
 
 Any opinions expressed within this e-mail are those of the individual and not 
 necessarily of Diamond Light Source Ltd. 
 
 Diamond Light Source Ltd. cannot guarantee that this e-mail or any 
 attachments are free from viruses and we cannot accept liability for any 
 damage which you may sustain as a result of software viruses which may be 
 transmitted in or with the message.
 
 Diamond Light Source Limited (company no. 4375679). Registered in England and 
 Wales with its registered office at Diamond House, Harwell Science and 
 Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom
 
 
 
 
 
 
 
 
 




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This e-mail and any attachments may contain confidential, copyright and or 
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are not the intended addressee or an authorised recipient of the addressee 
please notify us of receipt by returning the e-mail and do not use, copy, 
retain, distribute or disclose the information in or attached to the e-mail.

Any opinions expressed within this e-mail are those of the individual and not 
necessarily of Diamond Light Source Ltd. 

Diamond Light Source Ltd. cannot guarantee that this e-mail or any attachments 
are free from viruses and we cannot accept liability for any damage which you 
may sustain as a result of software viruses which may be transmitted in or with 
the message.

Diamond Light Source Limited (company no. 4375679). Registered in England and 
Wales with its registered office at Diamond House, Harwell Science and 
Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom

 

[ccp4bb] EMBO Practical Course in “High throughput Protein Production and Crystallization

[Dr. Isabel De Moraes]

Dear All, 

A gentle reminder that the deadline for the  practical course in 
High-troughtput Protein Production and Crystallisation  is 31st January. 

For more details, please see below.

For information: http://events.embo.org/13-htp-protein/index.html
For Registration:http://events.embo.org/13-htp-protein/application.html

With best Regards,
Isabel





On 14 Oct 2012, at 13:09, Dr. Isabel De Moraes wrote:

 Apologies for the previous email,  almost impossible to read.  Hopefully, 
 this one looks better.
 
 
 Dear All,
 
 We are happy to announce an EMBO Practical Course in  “High throughput 
 Protein Production and Crystallisation”
 
 The overall aim of the course will be to review the state-of-the-art in HTP 
 structural biology with an emphasis on methods to study complex targets, 
 including membrane proteins.
 
 The course is aimed at early career post-doctoral scientists and PhD students 
 in their second or third year of study.
 
 
 Practical training will be provided in:
 Structural bioinformatics
 High throughput cloning  expression in E. coli and mammalian cells
 Protein purification and biophysical characterisation
 High throughput protein crystallization
 High throughput data collection at synchrotrons
 
 Speakers:
 Lawrence Kelley  - Imperial College London
 David Drew  -  Imperial College London
 Imre Berger  - EMBL  Grenoble
 Chris Tate - University of Cambridge
 Naomi Chayen Imperial College London
 Opher Gileadi - University of Oxford
 Renaud Vincentelli - AFMB, Marseille
 Rob Meijers - EMBL, Hamburg
 Robert Gilbert - University of Oxford
 Scott Lesley - Genomics Institute of the Novartis Research Foundation- USA
 Joanne Nettleship - University of Oxford
 Louise Bird - University of Oxford
 Jon Diprose - University of Oxford
 Martin Walsh - Diamond Light Source
 Isabel De Moraes - Imperial College, London
 
 
 Venue: Research Complex at Harwell – UK
 Date: 15-23 May 2013
 Registration deadline: JANUARY 31ST, 2013
 Total Number of Participants limited to: 20 participants
 For information: http://events.embo.org/13-htp-protein/index.html
 For Registration: http://events.embo.org/13-htp-protein/application.html
 
 The organizers
 Ray Owens (OPPF-UK)
 Isabel Moraes (MPL)
 Martin Walsh (Diamond)
 
 
 
 
 -- 
 
 This e-mail and any attachments may contain confidential, copyright and or 
 privileged material, and are for the use of the intended addressee only. If 
 you are not the intended addressee or an authorised recipient of the 
 addressee please notify us of receipt by returning the e-mail and do not use, 
 copy, retain, distribute or disclose the information in or attached to the 
 e-mail.
 
 Any opinions expressed within this e-mail are those of the individual and not 
 necessarily of Diamond Light Source Ltd. 
 
 Diamond Light Source Ltd. cannot guarantee that this e-mail or any 
 attachments are free from viruses and we cannot accept liability for any 
 damage which you may sustain as a result of software viruses which may be 
 transmitted in or with the message.
 
 Diamond Light Source Limited (company no. 4375679). Registered in England and 
 Wales with its registered office at Diamond House, Harwell Science and 
 Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom
 
 
 
 
 
 
 
 
 




-- 

This e-mail and any attachments may contain confidential, copyright and or 
privileged material, and are for the use of the intended addressee only. If you 
are not the intended addressee or an authorised recipient of the addressee 
please notify us of receipt by returning the e-mail and do not use, copy, 
retain, distribute or disclose the information in or attached to the e-mail.

Any opinions expressed within this e-mail are those of the individual and not 
necessarily of Diamond Light Source Ltd. 

Diamond Light Source Ltd. cannot guarantee that this e-mail or any attachments 
are free from viruses and we cannot accept liability for any damage which you 
may sustain as a result of software viruses which may be transmitted in or with 
the message.

Diamond Light Source Limited (company no. 4375679). Registered in England and 
Wales with its registered office at Diamond House, Harwell Science and 
Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom

 

Re: [ccp4bb] Crystallography on BBC Radio 4 next week

[Dr. Isabel De Moraes]

Deal All,

Like many others, I also would like to encourage to listen the  “In Our Time” 
BBC Radio 4 program next week as part of the Bragg Centenary Celebration.

Melvyn will have Prof Mike Glazer (Oxford), Dr Chris Hammond (Leeds) and Prof 
Judith Howard (Durham) as guests.

A subject not to be missed to those who like to called of crystallographers.

Isabel Moraes




On 22 Nov 2012, at 14:28, Martin Walsh wrote:

Martyn, your selling in our time short...I'd say it's always b*$($ brilliant! 
-interesting to see who his guests will be next week to discuss the history of 
crystallography.
Bragg is the chancellor of Leeds university so it all fits rather well!
M


-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Martyn 
Winn
Sent: 22 November 2012 14:13
To: ccp4bb
Subject: Re: [ccp4bb] Crystallography on BBC Radio 4 next week

I'd just like to comment that In Our Time is usually pretty good. I caught the 
end of this morning's program on the Borgias. After hearing about murder, 
nepotism and incest, it was a bit disorientating to hear Melvyn announce 
crystallography as the next subject :)

m

-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Peter Keller
Sent: 22 November 2012 13:52
To: ccp4bb
Subject: Re: [ccp4bb] Crystallography on BBC Radio 4 next week

Dear all,

I have had several queries about this off-list, so here are some
clarifications.

I don't know if Melvyn Bragg is related to WL and WH Bragg. I doubt
it, but if he is maybe he will say. (Bragg is a fairly common name in
English.)

The normal subject matter of In Our Time is humanities-based (i.e.
art, history, philosophy etc.), but they deal with scientific or
mathematical subjects occasionally.

There are two live broadcasts, at 09:00 and 21:30 UK time. The later
one is a shortened version (edited from about 42 mins to about 28), so
better to listen to the earlier one if you can.

There are three options for listening other than the terrestrial radio
broadcast. All should work outside the UK (restrictions on using the
BBC iPlayer overseas mostly apply only to television, or on the radio
to certain special broadcasts such as some sporting events).

(i) Live on the internet at the times above: go to the programme page
at http://www.bbc.co.uk/programmes/b01p0s9s and click on the
LISTEN
icon (with a picture of a small loudspeaker) towards the upper right
of the page.

(ii) Using iPlayer after the second broadcast has finished. Go to the
programme page at http://www.bbc.co.uk/programmes/b01p0s9s and click
on the Listen now button on the left of the picture illustrating the
programme. In principle this should become available within a few
minutes of the end of the second broadcast (i.e. soon after 22:00 UK
time), but sometimes there is a longer delay of up to an hour. It
won't expire, so you can always listen on another day.

(iii) Download a podcast in mp3 format from
http://www.bbc.co.uk/podcasts/series/iot. This will become available
sometime after the second broadcast has finished, but it could be a
day or two afterwards. These podcasts also don't expire or disappear:
you can download them years after the original broadcasts.

Options (ii) and (iii) will get you the full rather than the shortened
version of the programme.

Regards,
Peter.

On Thu, 2012-11-22 at 10:30 +, Peter Keller wrote:
Dear all,

On Thursday next week, the BBC radio program In Our Time will be
discussing the history of crystallography. The link to the program
is http://www.bbc.co.uk/programmes/b01p0s9s. Unfortunately, it
doesn't
say
who the guests are, but they usually manage to find people with
something
interesting to say, even if the presenter Melvyn Bragg's grasp of
scientific
material isn't always that great.

I think that from outside the UK it is possible to listen live from
the link
above, and that a day or two after the broadcast a podcast will be
available
to download from http://www.bbc.co.uk/podcasts/series/iot.

Regards,
Peter.


--
Peter Keller Tel.: +44 (0)1223
353033
Global Phasing Ltd., Fax.: +44 (0)1223
366889
Sheraton House,
Castle Park,
Cambridge CB3 0AX
United Kingdom




-- 

This e-mail and any attachments may contain confidential, copyright and or 
privileged material, and are for the use of the intended addressee only. If you 
are not the intended addressee or an authorised recipient of the addressee 
please notify us of receipt by returning the e-mail and do not use, copy, 
retain, distribute or disclose the information in or attached to the e-mail.

Any opinions expressed within this e-mail are those of the individual and not 
necessarily of Diamond Light Source Ltd. 

Diamond Light Source Ltd. cannot guarantee that this e-mail or any attachments 
are free from viruses and we cannot accept liability for any damage which you 
may sustain as a result of software viruses which may be 

[ccp4bb] EMBO Practical Course in “High throughput Protein Production and Crystallization

[Dr. Isabel De Moraes]

Apologies for the previous email,  almost impossible to read.  Hopefully, this 
one looks better.


Dear All,

We are happy to announce an EMBO Practical Course in  “High throughput Protein 
Production and Crystallisation”

The overall aim of the course will be to review the state-of-the-art in HTP 
structural biology with an emphasis on methods to study complex targets, 
including membrane proteins.

The course is aimed at early career post-doctoral scientists and PhD students 
in their second or third year of study.


Practical training will be provided in:
Structural bioinformatics
High throughput cloning  expression in E. coli and mammalian cells
Protein purification and biophysical characterisation
High throughput protein crystallization
High throughput data collection at synchrotrons

Speakers:
Lawrence Kelley  - Imperial College London
David Drew  -  Imperial College London
Imre Berger  - EMBL  Grenoble
Chris Tate - University of Cambridge
Naomi Chayen Imperial College London
Opher Gileadi - University of Oxford
Renaud Vincentelli - AFMB, Marseille
Rob Meijers - EMBL, Hamburg
Robert Gilbert - University of Oxford
Scott Lesley - Genomics Institute of the Novartis Research Foundation- USA
Joanne Nettleship - University of Oxford
Louise Bird - University of Oxford
Jon Diprose - University of Oxford
Martin Walsh - Diamond Light Source
Isabel De Moraes - Imperial College, London


Venue: Research Complex at Harwell – UK
Date: 15-23 May 2013
Registration deadline: JANUARY 31ST, 2013
Total Number of Participants limited to: 20 participants
For information: http://events.embo.org/13-htp-protein/index.html
For Registration: http://events.embo.org/13-htp-protein/application.html

The organizers
Ray Owens (OPPF-UK)
Isabel Moraes (MPL)
Martin Walsh (Diamond)




-- 

This e-mail and any attachments may contain confidential, copyright and or 
privileged material, and are for the use of the intended addressee only. If you 
are not the intended addressee or an authorised recipient of the addressee 
please notify us of receipt by returning the e-mail and do not use, copy, 
retain, distribute or disclose the information in or attached to the e-mail.

Any opinions expressed within this e-mail are those of the individual and not 
necessarily of Diamond Light Source Ltd. 

Diamond Light Source Ltd. cannot guarantee that this e-mail or any attachments 
are free from viruses and we cannot accept liability for any damage which you 
may sustain as a result of software viruses which may be transmitted in or with 
the message.

Diamond Light Source Limited (company no. 4375679). Registered in England and 
Wales with its registered office at Diamond House, Harwell Science and 
Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom

 

[ccp4bb] EMBO Practical Course in “High throughput Protein Production and Crystallization

[Dr. Isabel De Moraes]

Dear All,
We are happy to announce an EMBO Practical Course in
“High throughput Protein Production and Crystallization”
The overall aim of the course will be to review the state-of-the-art in HTP 
structural biology with an emphasis on methods to study complex targets, 
including membrane proteins. The course is aimed at early 
career post-doctoral scientists and PhD students in their second or third year 
of study.
Practical training will be provided in: 


 Structural 
bioinformatics
High throughput cloning  expression in E. coli and mammalian cells
Protein purification and biophysical characterisation
High throughput protein crystallization
High throughput data collection at synchrotrons
Speakers:
Lawrence Kelley  - Imperial College London
David Drew  -  Imperial College London
Imre Berger  - EMBL  Grenoble
Chris Tate - University of Cambridge
Naomi Chayen Imperial College London
Opher Gileadi - University of Oxford
Renaud Vincentelli - AFMB, Marseille
Rob Meijers - EMBL, Hamburg
Robert Gilbert - University of Oxford
Scott Lesley - Genomics Institute of the Novartis Research Foundation- USA
Joanne Nettleship - University of Oxford
Louise Bird - University of Oxford
Jon Diprose - University of Oxford
Martin Walsh - Diamond Light Source
Isabel De Moraes - Imperial College, London

Venue: Research Complex at Harwell - UK
Registration deadline: JANUARY 31ST, 2013
Total Number of Participants limited to: 20 participants
For information: http://events.embo.org/13-htp-protein/index.html   

For Registration: 
http://events.embo.org/13-htp-protein/application.html

http://events.embo.org/13-htp-protein/application.htmlThe organizers
Ray Owens (OPPF-UK)
Isabel Moraes (MPL)
Martin Walsh (Diamond)






-- 

This e-mail and any attachments may contain confidential, copyright and or 
privileged material, and are for the use of the intended addressee only. If you 
are not the intended addressee or an authorised recipient of the addressee 
please notify us of receipt by returning the e-mail and do not use, copy, 
retain, distribute or disclose the information in or attached to the e-mail.

Any opinions expressed within this e-mail are those of the individual and not 
necessarily of Diamond Light Source Ltd. 

Diamond Light Source Ltd. cannot guarantee that this e-mail or any attachments 
are free from viruses and we cannot accept liability for any damage which you 
may sustain as a result of software viruses which may be transmitted in or with 
the message.

Diamond Light Source Limited (company no. 4375679). Registered in England and 
Wales with its registered office at Diamond House, Harwell Science and 
Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom

 

[ccp4bb] Symposium in Membrane Protein Crystallisation - UK

[Dr. Isabel De Moraes]

Dear All,

The Membrane Protein Laboratory (MPL) at Diamond Light Source is organising a 
Symposium in Membrane Protein Crystallisation on the 27th of September at the 
Research Complex at Harwell - UK

The registration is now open:
http://www.rc-harwell.ac.uk/symposium-in-membrane-protein-crystallization

There are a maximum of 75 places and the registration will be closed once all 
the places are taken.
Program
Symposium in Membrane Protein Crystallization
9:45 - 10:15arrival/registration
10:15 - 10:30   welcome
Session 1   Chair: Prof. Steve Mathews - Imperial College London
10:30 -11:00Introduction to Membrane Protein Crystallization
Isabel Moraes - Diamond - MPL
11:00 -11:30Stabilising Membrane Protein Samples for Crystallization
Paul Thaw and Jeanette Hobbs - Molecular Dimensions
11:30 - 12:00   lipidic Cubic Phase Technologies for Membrane Protein 
Structural Studies
Vadim Cherezov - The Scripps Research Institute - USA
12:00 - 12:30   Membrane Protein Nano and Macro Crystallization using Lipidic 
Sponge Phases
Linda Johansson - University of Gothenburg - Sweden
12:30 - 13:30   lunch
Session 2   Chair: Prof. Thomas Sorensen - Diamond Light Source
13:30 - 14:00   The Use of Bicelles in Membrane Protein Crystallization
Jeff Abramson - University of California, Los Angeles - USA
14:00 - 14:30   Current Trends in Alpha Helical Membrane Protein 
crystallization - The Rationale Behind MemGold, MemGold2 and MemAdvantage 
Screens
Simon Newstead - University of Oxford
14:30 - 15:00   Advances in Automated Imaging and Liquid Handling to Support 
Membrane Protein Research
Olivia Sleator - Rigaku
15:00 - 15:30   Crystallizing GPCRs for Drug Discovery
João Dias - Heptares
15:30 - 16:00   Break
session 3   Chair: Dr Alex Cameron - Imperial College London
16:00 - 16:30   Imaging Protein Crystals with Ultrafast Lasers
Ellen Gualtieri - Formulatrix
16:30 - 17:00   Exploiting Microfocused X-rays for Challenging Problems in MX
Robin Owen - Diamond Light Source
17:00 - 17:30   BLEND - The systematic Scaling and Merging of Multiple Datasets 
in Macromolecular crystallography
James Foadi - Diamond - MPL
17:30   Closing remarks



Sponsors
 
[http://www.diamond.ac.uk/Home/Events/Symposium-in-Membrane-Protein-Crystallization/mainColumnParagraphs/01/text_files/file2/molecular_dim1.jpg]
 http://www.moleculardimensions.com/  
[http://www.ttplabtech.com/assets/default/Site/en/img/logo.gif] 
http://www.ttplabtech.com/
 [http://www.formulatrix.com/images/formulatrix.png] 
http://www.formulatrix.com/   
[http://www.diamond.ac.uk/Home/Events/Symposium-in-Membrane-Protein-Crystallization/mainColumnParagraphs/01/text_files/file3/generon.png]
 http://www.generon.co.uk/
 
[http://www.diamond.ac.uk/Home/Events/Symposium-in-Membrane-Protein-Crystallization/mainColumnParagraphs/01/text_files/file/dibanner.png]
 http://www.douglas.co.uk/   
[http://www.rigaku.com/sites/default/files/Rigaku_0.jpg] 
http://www.rigaku.com/
 [http://www.rc-harwell.ac.uk/assets/site_files/img/header_logo.gif] 
http://www.rc-harwell.ac.uk/

[http://www.diamond.ac.uk/Home/Events/Symposium-in-Membrane-Protein-Crystallization/mainColumnParagraphs/01/text_files/file0/WellcomeTrust.png]http://www.wellcome.ac.uk/




[http://www.diamond.ac.uk/Home/Events/Symposium-in-Membrane-Protein-Crystallization/mainColumnParagraphs/01/text_files/file1/Image1.png]http://diamond.ac.uk/
 
[http://www3.imperial.ac.uk/2007templates/images/logo_imperial_college_london.png]
 http://www3.imperial.ac.uk/

We would be delighted to meet you at the symposium.


With best Regards,
Isabel Moraes

-
Dr. Isabel De Moraes, MRSC

Membrane Protein Laboratory Facility Co-ordinator
Membrane Protein Laboratory
Diamond Light Source Ltd,
Chilton, Didcot, Oxfordshire,
OX11 ODE, UK

Tel (direct): 01235 778664
--

--
This e-mail and any attachments may contain confidential, copyright and or 
privileged material, and are for the use of the intended addressee only. If you 
are not the intended addressee or an authorised recipient of the addressee 
please notify us of receipt by returning the e-mail and do not use, copy, 
retain, distribute or disclose the information in or attached to the e-mail.
Any opinions expressed within this e-mail are those of the individual and not 
necessarily of Diamond Light Source Ltd.
Diamond Light Source Ltd. cannot guarantee that this e-mail or any attachments 
are free from viruses and we cannot accept liability for any damage which you 
may sustain as a result of software viruses which may be transmitted in or with 
the message.
Diamond Light Source Limited (company no. 4375679). Registered in England and 
Wales with its registered office at Diamond House, Harwell Science and 
Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom

Re: [ccp4bb] Detergent and protein oligomerization

[Dr. Isabel De Moraes]

Dear Raji,

The best way to find out is to run a SEC-MALLS  (Size Exclusion Chomatography - 
Multi-Angle Laser Light Scattering) experiment.

Best,
Isabel

-
Dr. Isabel De Moraes, MRSC

Membrane Protein Laboratory Facility Co-ordinator
Membrane Protein Laboratory
Diamond Light Source Ltd,
Chilton, Didcot, Oxfordshire,
OX11 ODE, UK

Tel (direct): 01235 778664
--

From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Jacob Keller 
[j-kell...@fsm.northwestern.edu]
Sent: 21 June 2012 17:11
To: ccp4bb
Subject: Re: [ccp4bb] Detergent and protein oligomerization

First of all, isn't the choice either dimer or trimer, and second, as a 
protein-detergent complex (PDC), it would be very unlikely that a trimer of 99 
kD would run at 100 kD, although all is fair in love, war, and membrane 
proteins.

JPK

On Thu, Jun 21, 2012 at 10:50 AM, Raji Edayathumangalam 
r...@brandeis.edumailto:r...@brandeis.edu wrote:
Hi Everyone,

Sorry for the non-CCP4 post.

I have a very basic question about detergents, critical micelle concentration 
and behavior on gel filtration.

A 33kDa membrane protein was purified by gel filtration in a buffer containing 
0.4%(w/v) beta-NG (CMC: 6.5mM) and 0.046%(w/v)LDAO (CMC: 0.14mM).  So the 
concentrations of beta-NG and LDAO in the gel-filtration buffer are ~2X and 
~14X that of the CMCs of the respective detergents. The elution volume of the 
protein peak (plus detergent) on Superdex200 corresponds to a molecular mass of 
100kDa.

I think that the 100kDa mass above includes contributions from both the protein 
as well as the detergent micelles. If this is correct, is it then accurate to 
try to glean the oligomerization state of the protein (and conclude that it is 
a trimer or tetramer) without taking into account detergent micellar mass and 
its influence on elution volume?

How should one interpret the 100kDa mass estimate from the gel filtration?

Thanks.
Raji





--
Raji Edayathumangalam
Instructor in Neurology, Harvard Medical School
Research Associate, Brigham and Women's Hospital
Visiting Research Scholar, Brandeis University





--
***
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
email: j-kell...@northwestern.edumailto:j-kell...@northwestern.edu
***

[ccp4bb] Practical Membrane Protein Course in Brazil

[Dr. Isabel De Moraes]

Dear All,

The Laboratório Nacional de Biociências (Campinas-Brazil) and The Membrane 
Protein Laboratory (MPL) at Diamond Light Source are organising a Practical 
course in Structure and Function of Membrane Proteins.

The goal of the course is to introduce PhD students and post-docs to the basic 
principles of membranes proteins and the methods for production, structure 
solving and analyses of these proteins.

The course will run at The Laboratório Nacional de Biociências, Campinas-Brazil

The official language is english. 

The course suits attendees who are entirely new to the field as well as those 
who wish to acquire an in-depth understanding of the methodology.

The course will be limited to 20 people from Universities and Research 
Institutes from MERCOSUL.

Deadline for application: 30th June, 2012

For more information:
www2.lnbio.org.br/membraneproteinscourse


Best Wishes,
Isabel 

-
Dr. Isabel De Moraes, MRSC

Membrane Protein Laboratory Facility Co-ordinator
Membrane Protein Laboratory
Diamond Light Source Ltd,
Chilton, Didcot, Oxfordshire,
OX11 ODE, UK

Tel (direct): 01235 778664
--

Re: [ccp4bb] Automating liquid dispensing

[Dr. Isabel De Moraes]

The Liquidator 96 is at my opinion the best in the market. Is a manual system, 
but fast without problems related to cross contamination.
The electronic ones  (from other brands) at some point gives you problems with 
the electroninc system and the tips are not disposable (they take  long time 
between plates due to the tip washes). 
 
Regards,
Isabel
-

Dr. Isabel De Moraes, MRSC

Membrane Protein Laboratory Facilities Co-ordinator/Group Leader
Membrane Protein Laboratory,
Diamond Light Source Ltd,
Harwell Science and Innovation Campus,
Chilton, Didcot, Oxfordshire,
OX11 ODE, UK

Tel: 01235 778664
email: isabel.de-mor...@diamond.ac.uk

http://www.diamond.ac.uk/Science/MPL/default.htm
http://www.rc-harwell.ac.uk/

---



From: CCP4 bulletin board on behalf of Jim Fairman
Sent: Fri 3/11/2011 10:57 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Automating liquid dispensing


We also recently obtained a Liquidator 96 from Rainin.  Working well so far and 
way below the price of any of the competitors.


On Fri, Mar 11, 2011 at 5:41 PM, Engin Özkan eoz...@stanford.edu wrote:


Dear Stephen,

I did start a similar thread about a year ago. If you want to see the 
posts, check this out and the responses to it:

http://www.mail-archive.com/ccp4bb@jiscmail.ac.uk/msg14758.html

In my case, the winner was the Liquidator 96 from Rainin. It has proven 
to be very useful and too popular, and now it is being utilized for everything 
under the sun.

Best,
Engin

On 3/11/11 2:00 PM, Stephen McMahon wrote:


Hi -
We are in the market for a new liquid handling robot 
specifically for dispensing custom 96 well crystallisation screens.
What are people using these days? Likes / dislikes?
Many thanks,
Stephen




-- 
Engin Özkan
Post-doctoral Scholar
Howard Hughes Medical Institute
Dept of Molecular and Cellular Physiology
279 Campus Drive, Beckman Center B173
Stanford School of Medicine
Stanford, CA 94305
ph: (650)-498-7111





-- 
Jim Fairman, Ph D.
Post-Doctoral Fellow
National Institutes of Health - NIDDK 
The Buchanan Lab http://www-mslmb.niddk.nih.gov/buchanan/index.html 
Lab: 1-301-594-9229
E-mail: fairman@gmail.com james.fair...@nih.gov

Re: [ccp4bb] Liquid nitrogen resistant flooring

[Dr. Isabel De Moraes]

Dear Nick,

We start have the same sort of problem here at MPL.

However, we have already a few solutions to put in practice.

1-The research complex at Harwell has a excellent floor on the Liquid Nitrogen 
Room. The name of the floor tiles are Matlocker and you can find it from this 
site

www.jaymart.co.uk/search.php


2-Imperial College has solved the problem by acquiring a similar type of floor 
: Medium Rib Matting thickness 5 from the following supplier:
Key Industrial Equipment Ltd 
35 Black Moor Road Verwood Wimborne,
BH31 6AT United Kingdom
Fax: 0800 37303


In the case you are around Diamond you are welcome to have a look.

Regards,
Isabel

-

Dr. Isabel De Moraes, MRSC

Membrane Protein Laboratory Facilities Co-ordinator/Group Leader
Membrane Protein Laboratory,
Diamond Light Source Ltd,
Harwell Science and Innovation Campus,
Chilton, Didcot, Oxfordshire,
OX11 ODE, UK

Tel: 01235 778664
email: isabel.de-mor...@diamond.ac.uk

http://www.diamond.ac.uk/Science/MPL/default.htm
http://www.rc-harwell.ac.uk/

---



-Original Message-
From: CCP4 bulletin board on behalf of Nicholas Keep
Sent: Wed 2/16/2011 12:20 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Liquid nitrogen resistant flooring
 
Can anyone recommend a floor coating that passes category 2 containment (ie not 
wood) that is resistant to liquid 
nitrogen.  Ie you can fill dewars on without cracking.  Various solutions our 
estates people have fitted have all proved 
unsatisfactory.
Bets wishes
Nick
-- 

Prof Nicholas H. Keep
Executive Dean of School of Science
Professor of Biomolecular Science
Crystallography, Institute for Structural and Molecular Biology,
Department of Biological Sciences
Birkbeck,  University of London,
Malet Street,
Bloomsbury
LONDON
WC1E 7HX

email n.k...@mail.cryst.bbk.ac.uk
Telephone 020-7631-6852  (Room G57 Office)
   020-7631-6800  (Department Office)
Fax   020-7631-6803
If you want to access me in person you have to come to the crystallography 
entrance
and ring me or the department office from the internal phone by the door

[ccp4bb] Call for applications to the Membrane Protein Lab at Diamond

[Dr. Isabel De Moraes]

Call for applications to the Membrane Protein Lab at Diamond 

 

 

Dear Crystallographers,

 

The Membrane Protein Laboratory (MPL) at Diamond Light Source in
Oxfordshire (UK) is pleased to announce that is now open our call for
proposals. 

 

The MPL is a joint venture between the Diamond Light Source and Imperial
College London. It is a collaboration between Prof. So Iwata at Imperial
College and Dame Prof. Louise Johnson and Dr Gwyndaf Evans at Diamond.

 

The MPL is a state-of-the-art lab, fully equipped with purification
systems and crystallisation robots for membrane proteins.

 

The facility is open to applications from groups anywhere in the world.
Users are able to visit from a few days to a more extended periods
depending on their project.

 

For more information on MPL please see our website:

http://www.diamond.ac.uk/Home/MPL.html
http://www.diamond.ac.uk/Home/MPL.html 

 

and for 

 

information on how to apply and application forms please follow the
link:

http://www.diamond.ac.uk/Home/MPL/users.html
http://www.diamond.ac.uk/Home/MPL/users.html 

 

The deadline for this call is 23:59 on Friday 26th November 2010.  

 

 

If you have any questions or would like to discuss an application,
please contact the MPL group leader Dr Isabel Moraes by email:
i.mor...@imperial.ac.uk 

 

 

With best wishes,

Isabel Moraes