[ccp4bb] vacancy structural biologist/biophysicist faculty

[Elias Fernandez]

ASSISTANT/ASSOCIATE PROFESSOR POSITION IN EXPERIMENTAL

BIOPHYSICS/STRUCTURAL BIOLOGY AT THE UNIVERSITY OF TENNESSEE



The Department of Biochemistry & Cellular and Molecular Biology (BCMB) at
the University of Tennessee, Knoxville (UT) is soliciting applications for a
tenure‐track position at the rank of an Assistant or Associate Professor to
begin on August 1, 2018. BCMB (https://bcmb.utk.edu) has 40 active faculty
with core strengths in experimental and computational structural
biochemistry & biophysics, plant/microbial biology, and developmental
genetics. We seek applicants who use experimental structural or biophysical
approaches to study complex cellular, sub‐cellular, or molecular systems.



Assistant professors will be required to develop an innovative and
extramurally funded research program that complements existing areas of
research within the BCMB Department. Associate Professors will be expected
to have established and well-funded research programs. UT is located near
the Oak Ridge National Laboratory (ORNL) and candidates interested in
developing collaborative research programs with ORNL are encouraged to
apply. The successful candidate will benefit greatly from available
computational resources, the on-campus National Institute for Mathematical
and Biological Synthesis (NIMBioS) and the UT-ORNL Joint Institutes for
Computational Sciences, Biological Sciences, Advanced Materials, and Neutron
Sciences.  The Knoxville campus of the University of Tennessee is seeking a
candidate who has the ability to contribute in meaningful ways to the
diversity and intercultural goals of the University.



Applicants must have a doctorate (PhD or equivalent) and postdoctoral
experience in an appropriate discipline with evidence of high quality
research. Also important, is an interest in teaching biochemistry and/or
physical biochemistry at both the undergraduate and graduate levels.



Applicants must send a single document containing a cover letter, CV with a
full list of publications, statements of current and future research
interests, and of teaching philosophy in PDF format to
 experimentalbiophys...@utk.edu.
Applicants must also arrange to have at least three letters of
recommendation sent electronically to  
utkreferen...@utk.edu. The review of applications will begin in October 16,
2017, and will continue until the position is filled.





The University of Tennessee is an EEO/AA/Title VI/Title IX/Section
504/ADA/ADEA institution

in the provision of its education and employment programs and services.  All
qualified applicants will receive equal consideration for employment without
regard to race, color, national origin, religion, sex, pregnancy, marital
status, sexual orientation, gender identity, age, physical or mental
disability, or covered veteran status.

[ccp4bb] over-expression strategies

[Elias Fernandez]

Dear CCP4ers,

We've been struggling with little (nearly none) expression of our protein,
in both E coli and with in vitro transcription/translation methods. It
appears that our mRNA has high 2' structure with a low dG (theoretically
~760kcal/mol). If this is indeed the source of our problem, are there any
potential strategies to either disrupt the mRNA structure chemically (in E
coli or in vitro) or with  thermophile expression systems for expression at
higher temperatures?

Regards,

Elias

[ccp4bb] anti-His6 Ab

[Elias Fernandez]

Dear All, would you be able to recommend a primary anti-His6 Ab for western
blotting? Thus far, the ones we've used are not very specific to the
N-terminal hexahistidines and we pick up lots of background. Also, there's
one (others?) from Sigma that is conjugated to HRP, but costs ~$650. Thanks!
Elias

[ccp4bb] recombinant enterokinase

[Elias Fernandez]

Dear all,

Would anyone know of a source for recombinant enterokinase? 

Best,

Elias

Re: [ccp4bb] recombinant enterokinase

[Elias Fernandez]

Hi All, I misspoke - what I'm looking for is a source for an expression
system for recombinant enterokinase.

Elias

 

Subject: recombinant enterokinase 

 

Dear all,

Would anyone know of a source for recombinant enterokinase? 

Best,

Elias

[ccp4bb] still... problems with COOT and Pymol stereo

[Elias Fernandez]

 

 

Dear CCP4ers,

I'd sent this e-mail out a week or so ago. I'm still struggling with the 3D
kit and 2 monitors. 

I'd purchased a stereo monitor from Sharper Technology with a Planar SA2311W
stereo 3D LCD and the Nvidia 3D vision kit. However, I'm having a problem
with the dual monitor setup.  Everything works fine with Pymol and Coot when
using a single 3D capable monitor (Planar SA2311W) and NVIDIA 3D Vision
system (Quadro 4000But, whenever I plug in the second monitor the 3D image
(Coot and Pymol) becomes unstable (jittery - the molecule actually starts
jiggling). 

Oddly, I don't have the same problem with the example stereo pictures and
movies the company sends along. 

I'm using the MS Windows platform and I use the 2nd monitor for simultaneous
word-processing etc.

Any ideas for fixes?

Best,

Elias

[ccp4bb] Problems with COOT and Pymol stereo

[Elias Fernandez]

Dear CCP4ers,

I'd purchased a stereo monitor from Sharper Technology with a Planar SA2311W
stereo 3D LCD and the Nvidia 3D vision kit. However, I'm having a problem
with the dual monitor setup.  Everything works fine with Pymol and Coot when
using a single 3D capable monitor (Planar SA2311W) and NVIDIA 3D Vision
system (Quadro 4000).  However, whenever I plug in the second monitor the 3D
image (Coot and Pymol) becomes unstable (jittery - the molecule actually
starts jiggling). 

Oddly, I don't have the same problem with the example stereo pictures and
movies the company sends along. 

I'm using the MS Windows platform and I use the 2nd monitor for simultaneous
word-processing etc.

Any ideas for fixes?

Best,

Elias

[ccp4bb] Assistant Professor Vacancy

[Elias Fernandez]

ASSISTANT PROFESSOR: The Department of Biochemistry and Cellular and
Molecular Biology (BCMB) at the University of Tennessee, Knoxville seeks to
fill a tenure-track faculty position at the assistant professor level to
begin August 1, 2011 in the following area:

 

EXPERIMENTAL PHYSICAL BIOCHEMIST

 

Working in the area of nucleic acids or membranes. The successful applicant
will be expected to develop a first-class, externally funded research
program, to provide state-of-the-art training for graduate students and
postdoctoral researchers, and to contribute to the teaching mission of the
BCMB department at both the undergraduate and graduate levels.  Required
qualifications include a Ph.D. and postdoctoral experience in experimental
molecular biophysics of nucleic acids, membranes, nucleic acid-protein or
membrane-protein interactions, evidence of significant scientific
productivity, and a commitment to an integrated program of teaching and
research.  Current research strengths of BCMB relevant to this search
include Computational and Molecular Biophysics and Structural Biology. BCMB
also has strength in  Cell Biology, Genetics, Plant Biology, and
Neurobiology. The successful candidate will benefit from interactions with
strong research groups within UTK, access to supercomputing facilities
(Kraken) and Computational and Physical Sciences programs at Oak Ridge
National Laboratories (ORNL), which houses the new Spallation Neutron
Source. 

 

The University welcomes and honors people of all races, creeds, cultures,
and sexual orientations, and values intellectual curiosity, pursuit of
knowledge, and academic freedom and integrity.  Interested candidates should
send a cover letter, a resume, a description of research experience and of
the proposed research program, and arrange for three letters of reference to
be sent to: Engin Serpersu, Chair, Faculty Search Committee, BCMB
Department, M407 WLS, University of Tennessee, Knoxville, TN 37996-0840.
Review of applications will begin on October 1, 2010 and will continue until
the position is filled. 

 

All qualified applicants will receive equal consideration for employment and
admissions without regard to race, color, national origin, religion, sex,
pregnancy, marital status, sexual orientation, gender identity, age,
physical or mental disability, or covered veteran status.

 

Eligibility and other terms and conditions of employment benefits at The
University of Tennessee are governed by laws and regulations of the State of
Tennessee, and this non-discrimination statement is intended to be
consistent with those laws and regulations.   

 

In accordance with the requirements of Title VI of the Civil Rights Act of
1964, Title IX of the Education Amendments of 1972, Section 504 of the
Rehabilitation Act of 1973, and the Americans with Disabilities Act of 1990,
The University of Tennessee affirmatively states that it does not
discriminate on the basis of race, sex, or disability in its education
programs and activities, and this policy extends to employment by the
University.  

 

Inquiries and charges of violation of Title VI (race, color, national
origin), Title IX (sex), Section 504 (disability), ADA (disability), Age
Discrimination in Employment Act (age), sexual orientation, or veteran
status should be directed to the Office of Equity and Diversity (OED), 1840
Melrose Avenue, Knoxville, TN  37996-3560, telephone (865) 974-2498 (V/TTY
available) or 974-2440.  Requests for accommodation of a disability should
be directed to the ADA Coordinator at the Office of Equity and Diversity.

 

 

 

 

Elias J. Fernandez, PhD

Associate Professor

University of Tennessee

Biochemistry, Cellular  Molecular Biology

125 Austin Peay

Knoxville, TN 37996

Phone:(865) 974-4090

Fax:(865) 974-6306

e-mail: elias.fernan...@utk.edu