Dear Zhang yu,

I also found that same problem recently. I think is a small bug from Coot.
It will only copy NCS if the molecule's name you want to copy is molecule A.
So maybe will work if you rename your built chain as A.

Good luck!

Javier

On 31/03/11 00:16, zhang yu wrote:
Dear all,

I have two fold NCS in ASU. I could find phase by molecular replacement, but I have to build other chains by myself, since the model is not complete. During model building in coot, I built one chain in one NCS, and I merged this chain into the previous molecule. Now it is the "chain M'.

 When I tried to directly copy this chain to its NCS mate, It didn't work.

I followed the instruction posted before"http://www.mail-archive.com/coot@jiscmail.ac.uk/msg01984.html";.

What I did is
1. Change the master chain to chain M "Draw->NCS ghost control->change to chain M" (The terminal said 'there are no ghosts" when I change the master chain to chain M) 2. go to "Extension->NCS->copy chain->", coot ask to fill the master chain ID, and I typed "M"

Nothing happened. Could someone tell me what is the correct procedure to do it ?

Bye the way, for those chains present in both NCS maps, I modified one chain and applied the change to its NCS mate by using the same above procedure, and it worked.

Thanks

--
Yu Zhang
HHMI associate
Waksman Institute, Rutgers University
190 Frelinghuysen Rd.
Piscataway, NJ, 08904


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