Dear CCP4bb,
Can anyone point me to good reviews or books which describe how to crystallize protein-protein complexes (or anyone willing to share his/her experience)? In particular I'm interested in: (1) which tests to perform on your complex before you even start thinking about crystallization (2) what should you take in account when you start to crystallize (any other than 'use the Radaev and Sun' screen) (3) how to increase the success rate of crystallization (cross linking? co-expression?) (4) any alternative techniques when crystallization fails (any other than NMR) I would be very grateful! Thanks in advance, Maarten __________________________ Maarten Dewilde - PhD student Laboratory for Pharmaceutical Biology O&N2 - PB 824 - Herestraat 49 3000 Leuven <mailto:[EMAIL PROTECTED]> [EMAIL PROTECTED] tel: +32 16 323434 fax: +32 16 323460 Disclaimer: http://www.kuleuven.be/cwis/email_disclaimer.htm
