[ccp4bb] AutoSol invalid MTZ column_types error

[Mintu Chandra]

Dear all,


I am getting an error message "Invalid MTZ column_types for the given
miller_array." while I try to run AutoSol. The last line in the log is
"Adding array ['N(+)', 'N(-)'] to output file with type II".
Do you have any suggestion how to fix this error?

Thanks a lot in advance. Best,

Mintu

[ccp4bb] relative domain motion calculation...

[Mintu Chandra]

Dear All,
I am working with a protein containing tandem domains, connected with
3-4 residue linker. I want to calculate the relative
motion/orientation of one domain with respect to the hinge region as
well as with respect to the other domain. Both the domains have very
less seq. identity (20%). Please suggest some web server to calculate
the same.

Thanks,

Mintu

[ccp4bb] protocol for seleno-methionine incorporation...

[Mintu Chandra]

Dear all,
Is there any standard protocol to know the number of incorporated
seleno-methionine in a protein ??? I am growing one of the protein in
minimal media for phase determination.
I am using mass spectrometry facility to know the incorporated
seleno-methionine but I wanted to know whether I should use the purified
protein  or  digested protein for mass spectrometry.

Thanks,

Mintu Chandra.

-- 
Mintu Chandra
Senior Research Fellow
IISER BHOPAL
mi...@iiserb.ac.in
+918085288853

[ccp4bb] Align linux version

[Mintu Chandra]

Dear CCP4 users,

We are trying to align two different structures. It would be helpful
for us if anybody has linux compiled align version?

Regards,

Mintu Chandra