[ccp4bb] Molrep and Refmac5 errors

2013-10-15 Thread Rajiv K Bedi 
Dear all,

I recently started using CCP4i (Windows 8) software for data processing and 
model building but I get the following error messages whenever I use molrep and 
refmac5:

MOLREP
#CCP4I TERMINATION STATUS 0  MOLREP(ccp4):  Error in label assignments in 
LABIN
#CCP4I TERMINATION TIME 08 Oct 2013  16:27:56
#CCP4I MESSAGE Task failed

REFMAC5
#CCP4I TERMINATION STATUS 0  Program received signal 11 (SIGSEGV): Segmentation 
fault.
#CCP4I TERMINATION TIME 08 Oct 2013  16:26:50
#CCP4I MESSAGE Task failed

I get those error with every data set I tried, therefore, I would be really 
appreciative if you could help me with it.

Thanks,
Rajiv

Re: [ccp4bb] Improve diffraction ...any ideas?

2013-05-24 Thread Rajiv K Bedi 
I think you can soak another protein into a protein crystal if it is small 
enough to pass through water channels, I guess.

More info:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2483499/

Re: [ccp4bb] Improve diffraction ...any ideas?

2013-05-23 Thread Rajiv K Bedi 
Dear Umri,

I think the main problem is co-crystallization.

What I would do is crystallize protein and antibody separately and then soak 
protein crystals into reservoir solution containing antibody or vice versa.
And do try to get crystals from different conditions which may alter the space 
group and thereby improve diffraction quality, hopefully.

All the best,
Rajiv