Re: [ccp4bb] Best (Suitable) Mac Laptop configuration for protein Xtallography
Keep in mind the updated 15 MacBook Pro is slated to come out soon (sounds like early summer). Probably won't have more RAM but is supposed to have a new processor. If you can hold off for a couple months I would suggest waiting as these haven't been updated in almost a year. Rob On Thu, Apr 2, 2015 at 7:03 AM, xaravich ivan xaravich.i...@gmail.com wrote: Hello everyone, I am planing to buy a new Mac laptop (price no bar) which will let me run all xtallographic (CCP4 and Phenix) and reasonable Rosetta Molecular Modelling (1000 to 1 decoys) softwares smoothly. What in your opinion is the best configuration. (RAM, memory, number and speed of processors, graphics card etc.) Also I am buying a Mac display separately so that I have a big screen for easy visualization of models, COOT etc. I know that powerful Mac desktops can make life much easier but here I am specifically interested in Mac laptops only. As always thanks in advance and I will post all the suggestions anonymously for others with same query. ivan
[ccp4bb] Off Topic:crystallization in the presence of glycerol
Dear All, I am working with a protein that requires 10% glycerol throughout the purification to keep it soluble. I have been very worried that having glycerol in my protein solution when I am trying to crystallize it will prevent me from obtaining crystals. I am curious to see if others have successfully obtained crystals of proteins that required glycerol to keep them soluble and if so what concentration of glycerol could you use? Should I be concerned about this or am I worrying too much? Thanks for your input Rob -- Robert J.Gruninger B.Sc. PhD Student Department of Chemistry Biochemistry University of Lethbridge, 4401 University Dr. Lethbridge, AB, Canada, T1K 3M4 Phone:(403)329-2746 Fax:(403)329-2057
Re: [ccp4bb] crystal with precipitation
Hi Shivesh I had something like this recently and found that changing the pH stopped the precipitation and the size of the crystals improved. If the crystals are large enough even if there is ppt present I would still try and collect data on them. This is likely not the case for you or you wouldn't be writing in:) hope this helps Rob Dear all I am trying to crystallize a 7kDa protein using MPD as a precipitant at 16C.I have got small florets at 55-65% of MPD in 3-4 days.The problem is that the drop is precipitating in one day only and the crystals are coming with precipitation.I have added 5% glycerol and 100mM of Nacl as an additive with the mother liquor to avoid precipitation.But,the precipitation is still there.The volume of the mother liquor is 500 microlt.The drop size is 2+2.I welcome all the suggestions regarding avoiding the precipitation.Thanx is advance. Shivesh kumar -- Robert J.Gruninger B.Sc. PhD Student Department of Chemistry Biochemistry University of Lethbridge, 4401 University Dr. Lethbridge, AB, Canada, T1K 3M4 Phone:(403)329-2746 Fax:(403)329-2057
