[ccp4bb] Open position for Staff Scientist
All - Just want to draw your attention to a staff scientist position at the Beckman Research Institute at City of Hope. City of Hope has a proven track record for translating our discoveries into life-saving medicines, playing a fundamental role in the development of recombinant insulin, humanization of monoclonal antibodies and CAR T cell therapies (with core patents covering each). The institute has over 27 core facilities with three FDA-approved/CA-licensed GMP facilities (small molecules, biologics and cellular therapies). Over the years, it has built a storn culture of communication between the clinicians and research faculty and a sense of urgency to treat and cure patients with cancer and diabetes. The ideal candidate will be a self-starter that likes to work with other PIs across the institute. Projects that come to the core vary widely from de novo structure determination to protein-protein interactions to fragment screening and rational drug design. X-ray crystallography experience is absolutely required. Outstanding communication skills are also a must. The core houses a Rikagu 007HF, a mosquito crystallization robot, and two Formulatrix's imagers. In addition, the core has a Biacore T200, Water nanoITC, a Beckman XLI, Jasco 810 CD/fluorimeter and a Kinexa3000. All instruments are under vendor service contracts - our focus is medicine, not maintenance. The core also provides protein production using multiple expression systems as well as purification. The candidate will work closely with other core staff as well as other City of Hope cores involved with translational medicine (i.e., computational, HTS, synthetic chemistry, and mass spectrometry cores.) City of Hope is located in Duarte, just a few miles away from Pasadena and close to all the major attractions of Los Angeles and Southern California, yet the campus is less than 10 minutes away from the San Gabriel mountains and numerous hiking trails (one as high as 10,000ft). The salary and benefits are highly competitive, the work is highly disciplinary and the commitment to patient care unites us. Please apply at: https://chm.tbe.taleo.net/chm04/ats/careers/v2/viewRequisition?org=CITYOFHOPE=40=14640 Best, John _ John C. Williams, PhD Professor, Dept. of Molecular Medicine; Co-Director, Drug Discovery and Structural Biology Core; Member, Cancer Immunotherapeutics Program, NCI Designated Comprehensive Cancer Center; Beckman Research Institute at City of Hope 1710 Flower Street Duarte CA 91010 *SECURITY/CONFIDENTIALITY WARNING: This message and any attachments are intended solely for the individual or entity to which they are addressed. This communication may contain information that is privileged, confidential, or exempt from disclosure under applicable law (e.g., personal health information, research data, financial information). Because this e-mail has been sent without encryption, individuals other than the intended recipient may be able to view the information, forward it to others or tamper with the information without the knowledge or consent of the sender. If you are not the intended recipient, or the employee or person responsible for delivering the message to the intended recipient, any dissemination, distribution or copying of the communication is strictly prohibited. If you received the communication in error, please notify the sender immediately by replying to this message and deleting the message and any accompanying files from your system. If, due to the security risks, you do not wish to receive further communications via e-mail, please reply to this message and inform the sender that you do not wish to receive further e-mail from the sender. (LCP301) To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1 To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
[ccp4bb] Postdoctoral Position Open
This is an extraordinary opportunity join the efforts to develop a new class of protein therapeutics to cure Type 1 Diabetes in laboratories of Professors John Williams, Enrique Casimiro, and Bart Roep at the Beckman Research Institute and the Diabetes & Metabolism Research Institute at the City of Hope, a world class institute that played essential roles in the development and translation of recombinant insulin and humanization of monoclonal antibodies and continues to lead in the development CAR T cell and other novel therapeutic approaches. The project will involve the rational design, production and characterization of novel biologics using technologies pioneered in the Williams lab and discoveries made in the Roep/Casimiro Labs. It is anticipated that the successful applicants will have the opportunity to participate in and translate their leads through pre-clinical development (see https://www.cityofhope.org/news/city-of-hope-sets-new-goal-for-type-1-diabetes-cure). Responsibilities: Protein engineering, production, purification Biophysical characterization (SPR, ITC, AUC, CD) Assay development, interpretation, and troubleshooting Minimum Education and Skills Required for Consideration: PhD in biochemistry, molecular biology, protein chemistry, biochemical engineering or related field. Experience with protein expression and purification Excellent written and verbal communication skills Good problem solving skills Ability to conduct independent research Ability to supervise others and work effectively in a team Industrial experience a plus Apply at: https://chm.tbe.taleo.net/chm04/ats/careers/v2/viewRequisition?org=CITYOFHOPE=49=10137 - -SECURITY/CONFIDENTIALITY WARNING- This message (and any attachments) are intended solely for the individual or entity to which they are addressed. This communication may contain information that is privileged, confidential, or exempt from disclosure under applicable law (e.g., personal health information, research data, financial information). Because this e-mail has been sent without encryption, individuals other than the intended recipient may be able to view the information, forward it to others or tamper with the information without the knowledge or consent of the sender. If you are not the intended recipient, or the employee or person responsible for delivering the message to the intended recipient, any dissemination, distribution or copying of the communication is strictly prohibited. If you received the communication in error, please notify the sender immediately by replying to this message and deleting the message and any accompanying files from your system. If, due to the security risks, you do not wish to receive further communications via e-mail, please reply to this message and inform the sender that you do not wish to receive further e-mail from the sender. (LCP301) -
[ccp4bb] Immediate opening...
Please see: http://chm.tbe.taleo.net/chm04/ats/careers/requisition.jsp?org=CITYOFHOPE=1=7760 We anticipate filling the position immediately. No need to reapply if you have do so already. *** Crystallographer and Computational Modeler Position Summary and Key Responsibilities: This is an extraordinary opportunity to join the efforts to develop a new class of immunotherapeutics in laboratory of Professor John Williams at the Beckman Research Institute at the City of Hope, a world class institute that played essential roles in the development and translation of recombinant insulin and humanization of monoclonal antibodies and continues to lead in the development CAR T cell and other novel therapeutic approaches. The position will be one of five new research scientist positions in Professor Williams' laboratory funded by an innovative biotechnology company focused on the discovery and development of novel best-in-class immunotherapeutics for the treatment of cancer. The breakthrough technology that underpins the research platform was pioneered in the Williams lab. It is anticipated that the successful applicants may have the opportunity to transition into the drug discovery or pre-clinical development organizations of the biotechnology company in the future. Responsibilities: Crystallization Structure determination Modeling and Computation Minimum Education and Skills Required for Consideration: PhD in biophysics, structural biology, biochemistry or related field Protein purification and analytical characterization All aspects of protein crystallography – crystallization, crystallization optimization, data collection (in-house/synchrotron), structure determination and refinement, structure analysis Computational modeling (familiarity with Schrodinger or other packages) Independence Excellent written and verbal communication skills Good problem solving skills Ability to work effectively in a team Post-Doctoral and/or Industrial experience is desirable About City of Hope City of Hope, an innovative biomedical research, treatment and educational institution with over 4000 employees, is dedicated to the prevention and cure of cancer and other life-threatening diseases and guided by a compassionate, patient-centered philosophy. Founded in 1913 and headquartered in Duarte, California, City of Hope is a remarkable non-profit institution, where compassion and advanced care go hand-in-hand with excellence in clinical and scientific research. City of Hope is a National Cancer Institute designated Comprehensive Cancer Center and a founding member of the National Comprehensive Cancer Network, an alliance of the nation’s 20 leading cancer centers that develops and institutes standards of care for cancer treatment. City of Hope is an equal opportunity employer. All qualified applicants will receive consideration for employment without regard to race, religion, color national origin, sex, age, status as a protected veteran, or status as a qualified individual with disability. - *SECURITY/CONFIDENTIALITY WARNING: This message and any attachments are intended solely for the individual or entity to which they are addressed. This communication may contain information that is privileged, confidential, or exempt from disclosure under applicable law (e.g., personal health information, research data, financial information). Because this e-mail has been sent without encryption, individuals other than the intended recipient may be able to view the information, forward it to others or tamper with the information without the knowledge or consent of the sender. If you are not the intended recipient, or the employee or person responsible for delivering the message to the intended recipient, any dissemination, distribution or copying of the communication is strictly prohibited. If you received the communication in error, please notify the sender immediately by replying to this message and deleting the message and any accompanying files from your system. If, due to the security risks, you do not wish to receive further communications via e-mail, please reply to this message and inform the sender that you do not wish to receive further e-mail from the sender. (fpc5p) -
Re: [ccp4bb] KD of dimerization, off topic
Sedimentation equilibrium or sedimentation velocity experiments by analytical centrifugation is the best method for this. From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] On Behalf Of FOOS Nicolas [nicolas.f...@synchrotron-soleil.fr] Sent: Friday, February 14, 2014 12:25 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] KD of dimerization, off topic I agree with Dave, and i suggest one more method to estimate Kd, The intrinsic fluorescence of proteins thanks to the aromatic chain side. Maybe it's also possible to have an estimation with native gels if you use prot A concentration as fixed and B protein concentration as variable. I am not sure. De : CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] de la part de David Briggs [drdavidcbri...@gmail.com] Envoyé : vendredi 14 février 2014 09:13 À : CCP4BB@JISCMAIL.AC.UK Objet : Re: [ccp4bb] KD of dimerization, off topic Hi Careina, I'm not sure you can assume that the ratio of monomer and dimer will stay constant through the column - as you say, the protein is diluted during the run, the ratio will change, unless you have a super tight dimer - which clearly you do not. Also, as the mass and the molar extinction coefficient will both double in the dimer, the relationship between absorbance and concentration will be unchanged. Typically, such these sorts of questions are answered (at least me) by equilibrium analytical centrifugation. Hth, Dave On 14 Feb 2014 08:03, Careina Edgooms careinaedgo...@yahoo.commailto:careinaedgo...@yahoo.com wrote: Dear CCP4 board I have a protein that exists in equilibrium between monomer and dimer and I'm trying to calculate KD using size exclusion. The problem is that the column dilutes my sample so that if I put 20 uM on to the column, I only recover 0.5 uM in dimer fraction and 2 uM in monomer fraction. I am getting confused as to how to plot my KDs. Do I not regard my initial concentrations at all and work only with the final concentrations that come off the column? I would plot [monomer] squared vs [dimer] and I will assume that the ratio of monomer to dimer will stay constant as the protein passes through the column. (also I would calculate [dimer] using 2x monomer extinction coefficient) Does this seem a reasonable way to calculate KDs and reasonable argument? Also I am looking for good references for calculating Kds when dealing with dimerization Thanks and sorry for off topic question Careina - *SECURITY/CONFIDENTIALITY WARNING: This message and any attachments are intended solely for the individual or entity to which they are addressed. This communication may contain information that is privileged, confidential, or exempt from disclosure under applicable law (e.g., personal health information, research data, financial information). Because this e-mail has been sent without encryption, individuals other than the intended recipient may be able to view the information, forward it to others or tamper with the information without the knowledge or consent of the sender. If you are not the intended recipient, or the employee or person responsible for delivering the message to the intended recipient, any dissemination, distribution or copying of the communication is strictly prohibited. If you received the communication in error, please notify the sender immediately by replying to this message and deleting the message and any accompanying files from your system. If, due to the security risks, you do not wish to receive further communications via e-mail, please reply to this message and inform the sender that you do not wish to receive further e-mail from the sender. (fpc5p) -
[ccp4bb] West Coast Protein Crystallography Workshop 21!
All, The West Coast Protein Crystallography Workshop is fast approaching. We have a pretty full house with an outstanding line up of talks and posters! If you would still like to participate we can accommodate more people (see wcpcw.org). That said, we absolutely need your abstract first thing tomorrow morning to get it in the program. Only posters presentations are still available. We will be distributing the schedule tomorrow. Looking forward to seeing everybody. Best, John WCPCW 21 Organizer - *SECURITY/CONFIDENTIALITY WARNING: This message and any attachments are intended solely for the individual or entity to which they are addressed. This communication may contain information that is privileged, confidential, or exempt from disclosure under applicable law (e.g., personal health information, research data, financial information). Because this e-mail has been sent without encryption, individuals other than the intended recipient may be able to view the information, forward it to others or tamper with the information without the knowledge or consent of the sender. If you are not the intended recipient, or the employee or person responsible for delivering the message to the intended recipient, any dissemination, distribution or copying of the communication is strictly prohibited. If you received the communication in error, please notify the sender immediately by replying to this message and deleting the message and any accompanying files from your system. If, due to the security risks, you do not wish to receive further communications via e-mail, please reply to this message and inform the sender that you do not wish to receive further e-mail from the sender. (fpc5p) -
[ccp4bb] West Coast Protein Crystallography Workshop 21
All, We will be finalizing the program for the workshop on Monday, Feb 25th. The Monterey Plaza and Spa has confirmed that we can accommodate a few more people. So, if you are interested in attending and would like to be considered for an oral presentation or would like to present a poster, please register and submit an abstract no later than Monday, 12 noon (PST). The meeting will be March 17th-20th with Wayne Hendrickson as the Keystone speaker! Best, John wcpcw.org - *SECURITY/CONFIDENTIALITY WARNING: This message and any attachments are intended solely for the individual or entity to which they are addressed. This communication may contain information that is privileged, confidential, or exempt from disclosure under applicable law (e.g., personal health information, research data, financial information). Because this e-mail has been sent without encryption, individuals other than the intended recipient may be able to view the information, forward it to others or tamper with the information without the knowledge or consent of the sender. If you are not the intended recipient, or the employee or person responsible for delivering the message to the intended recipient, any dissemination, distribution or copying of the communication is strictly prohibited. If you received the communication in error, please notify the sender immediately by replying to this message and deleting the message and any accompanying files from your system. If, due to the security risks, you do not wish to receive further communications via e-mail, please reply to this message and inform the sender that you do not wish to receive further e-mail from the sender. (fpc5p) -
[ccp4bb] West Coast Protein Crystallography Workshop
Dear All, The West Coast Protein Crystallography Workshop is coming up fast. Consistent with previous WCPCW meetings, there will be ample discussion of methods, structural analyses and new structures. The keynote speaker will be Wayne Hendrickson. He will be presenting some of his recent work on SAD phasing and its application to important biological problems including membrane proteins. Andy Karplus will also give a talk on his and Kay Diederichs' CC1/2. That said, the focus will be on postdoctoral and predoctoral presentations. The meeting will be held at the Monterey Plaza and Spa, March 17th to 20th and the registration is all-inclusive (e.g., meeting fees, room, board and social hours are included). There is still space available for those who are interested in attending. See www.wcpcw.org or contact me directly. For those who have already registered, please send your abstract asap if you have yet to do so. We need to compile the program, assign speaking slots, etc... Best, John John C. Williams, PhD Assoc. Professor, Dept. of Molecular Medicine, Beckman Research Institute; Co-Director, Drug Discovery and Structural Biology Core, NCI Comprehensive Cancer Center; City of Hope 1710 Flower St., Duarte, CA 91016 (626) 256 4673 (x60227) For messages and meetings: Ariadno Bello, Administrative Assistant abe...@coh.org (626) 256 4673 (x60226) - *SECURITY/CONFIDENTIALITY WARNING: This message and any attachments are intended solely for the individual or entity to which they are addressed. This communication may contain information that is privileged, confidential, or exempt from disclosure under applicable law (e.g., personal health information, research data, financial information). Because this e-mail has been sent without encryption, individuals other than the intended recipient may be able to view the information, forward it to others or tamper with the information without the knowledge or consent of the sender. If you are not the intended recipient, or the employee or person responsible for delivering the message to the intended recipient, any dissemination, distribution or copying of the communication is strictly prohibited. If you received the communication in error, please notify the sender immediately by replying to this message and deleting the message and any accompanying files from your system. If, due to the security risks, you do not wish to receive further communications via e-mail, please reply to this message and inform the sender that you do not wish to receive further e-mail from the sender. (fpc5p) -
