[ccp4bb] Postdoc position in San Antonio, TX
*NIH-funded Postdoctoral Associate position in Structural Virology at the University of Texas Health Science Center, San Antonio* The laboratory of Dr. Yogesh Gupta (http://ccri.uthscsa.edu/YGupta.html) is seeking an enthusiastic Postdoctoral Associate to study the structures and specificity of RNA modification enzymes. We will mainly use X-ray crystallography and cryo-EM to study the mechanisms and develop small molecule antiviral candidates. We provide a rich multi-disciplinary environment and access to cutting-edge technologies to our trainees. Further details of our recent work can be found here: Elife. 2022 Jan 21;11:e67150. doi: 10.7554/eLife.67150 <https://elifesciences.org/articles/67150>. Nature Communications. 2021 Jun 2; 12: 3287. PMID: 34078893. doi: 10.1038/s41467-021-23594-y <https://www.nature.com/articles/s41467-021-23594-y> Nature Communications. 2020 Jul 24;11(1):3718. PMID: 32709886. doi: 10.1038/s41467-020-17496-8 <https://www.nature.com/articles/s41467-020-17496-8> Preference will be given to applicants with demonstrated experience in protein purification from insect or mammalian cells, protein/nucleic acid biochemistry, X-ray crystallography, and cryo-EM. Candidates are expected to possess strong organizational and interpersonal skills and the ability to work independently but also effectively and collaboratively as part of a team. An ideal candidate should hold (or soon expect to) a Ph.D. or equivalent degree in biochemistry/biophysics/structural biology. The Greehey Children’s Cancer Research Institute (GCCRI) is a unique, specialized cancer research center of UT Health Science Center San Antonio, focusing on basic and translational research. It occupies a state-of-the-art 100,000 sq. foot research facility on the university’s Greehey Academic and Research Campus. The GCCRI has significant external funding, a large endowment from the state, and links to philanthropic entities. San Antonio is the nation’s seventh-largest city and is located at the edge of the beautiful Texas Hill Country. San Antonio offers a rich, multicultural community, affordable cost of living, excellent weather, and a thriving biomedical industry. There is no state income tax in Texas. Salary is competitive and commensurate with experience. Interested candidates can apply by sending a short description of their research accomplishments and a CV with the contact information of 3 references to Dr. Yogesh Gupta (email: gup...@uthscsa.edu). *All Postdoctoral appointments are designated as security-sensitive positions. UT Health San Antonio is an Equal Opportunity/Affirmative Action Employer, including protected veterans and persons with disabilities.* -- Yogesh Gupta, Ph.D. Associate Professor Department of Biochemistry and Structural Biology University of Texas Health Science Center at San Antonio 8403 Floyd Curl Drive, San Antonio, TX, USA 78229 http://ccri.uthscsa.edu/YGupta.html To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
[ccp4bb] Postdoc position at University of Texas Health Science Center at San Antonio
My research group at UT Health San Antonio <https://gccri.uthscsa.edu/lab/gupta/> (UTHSA) is looking for a creative and self-motivated Postdoctoral Fellow scientist to study the structures and mechanisms of mRNA modification enzymes. We will use X-ray crystallography, cryoEM, and enzyme biochemistry to address basic mechanistic questions and develop strategies for rational drug development. Below are some of our recent publications: eLife. 2022 Jan 21;11. PMID: 35060905. doi.org/10.7554/eLife.67150 Nature Communications. 2021 Jun 2; 12: 3287. PMID: 34078893. doi: 10.1038/s41467-021-23594-y <https://www.nature.com/articles/s41467-021-23594-y> Nature Communications. 2020 Jul 24;11(1):3718. PMID: 32709886. doi: 10.1038/s41467-020-17496-8 <https://www.nature.com/articles/s41467-020-17496-8> The Greehey Children’s Cancer Research Institute (GCCRI) is a unique, specialized cancer research center focusing on basic and translational research in childhood cancer and occupies a state-of-the-art 100,000 sq. foot research facility on the UTHSA’s Greehey Academic Campus. San Antonio is the nation’s seventh-largest city and is located at the edge of the beautiful Texas Hill Country. The city offers a rich, multi-cultural community, affordable cost of living, excellent weather, and a thriving biomedical industry. Applicants with prior experience in macromolecular crystallography and a strong interest in cryoEM studies are encouraged to apply. Interested candidates can submit their application (CV, a brief description of their accomplishments, and contacts of 3 references) directly by email to me (Email: gup...@uthscsa.edu). Yogesh Yogesh Gupta, PhD Associate Professor University of Texas Health Science Center at San Antonio Department of Biochemistry & Structural Biology Greehey Children’s Cancer Research Institute 8403 Floyd Curl Drive, San Antonio, TX, USA 78229 Email: gup...@uthscsa.edu http://ccri.uthscsa.edu/YGupta.html To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
[ccp4bb] Fwd: Postdoctoral Fellow position at University of Texas Health Science Center San Antonio
My research group at UT Health San Antonio <https://gccri.uthscsa.edu/lab/gupta/> is looking for a creative and self-motivated Postdoctoral Fellow to study the structures and mechanisms of nucleic acid enzymes. We will use X-ray crystallography, cryoEM, and enzyme biochemistry to address basic mechanistic questions and develop drug candidates. Below are some of our recent publications: *eLife. *2022 Jan 21;11. PMID: 35060905. doi.org/10.7554/eLife.67150 *Nature Communications*. 2021 Jun 2; 12: 3287. PMID: 34078893. doi: 10.1038/s41467-021-23594-y <https://www.nature.com/articles/s41467-021-23594-y> *Nature Communications*. 2020 Jul 24;11(1):3718. PMID: 32709886. doi: 10.1038/s41467-020-17496-8 <https://www.nature.com/articles/s41467-020-17496-8> Applicants with prior experience in macromolecular crystallography and a strong interest in cryoEM studies and drug discovery are encouraged to apply. We already have crystals and cryoEM datasets available for an immediate start. The Greehey Children’s Cancer Research Institute (GCCRI) is a unique, specialized cancer research center focusing on basic and translational research in childhood cancer and occupies a state-of-the-art 100,000 sq. foot research facility on the university’s Greehey Academic and Research Campus. The GCCRI has significant external funding, a large endowment from the state, and links to philanthropic entities. San Antonio is the nation’s seventh-largest city and is located at the edge of the beautiful Texas Hill County. San Antonio offers a rich, multi-cultural community, affordable cost of living, excellent weather, and a thriving biomedical industry. Interested candidates can submit their application (CV, a brief description of their accomplishments, and contacts of 3 references) directly by email to me (Email: gup...@uthscsa.edu) Yogesh Yogesh Gupta, PhD Associate Professor of Biochemistry & Structural Biology Greehey Children's Cancer Research Institute University of Texas Health Science Center San Antonio 8403 Floyd Curl Drive, San Antonio, TX, USA 78229 Email: gup...@uthscsa.edu http://ccri.uthscsa.edu/YGupta.html To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
[ccp4bb] Postdoctoral Fellow position at University of Texas Health Science Center San Antonio
My research group at UT Health San Antonio <https://gccri.uthscsa.edu/lab/gupta/> is seeking to immediately hire a Postdoctoral Fellow who can work on the structures and mechanisms of human RNA/DNA/chromatin modification complexes. We will use X-ray crystallography, cryo-EM, and enzyme biochemistry to address basic mechanistic questions and develop anticancer agents for the treatment of pediatric tumors. Below are some of our recent publications: 1. eLife. 2022 Jan 21;11. doi: 10.7554/eLife.67150. PubMed PMID: 35060905. doi.org/10.7554/eLife.67150 2. Nature Communications. 2021 Jun 2; 12: 3287. PMID: 34078893. doi: 10.1038/s41467-021-23594-y <https://www.nature.com/articles/s41467-021-23594-y> 3. Nature Communications. 2020 Jul 24;11(1):3718. PMID: 32709886. doi: 10.1038/s41467-020-17496-8 <https://www.nature.com/articles/s41467-020-17496-8> Candidates with demonstrated experience in X-ray crystallography and a strong interest in cryo-EM studies and drug discovery are especially encouraged to apply. We already have crystals and cryo-EM datasets available for an immediate start. The Greehey Children’s Cancer Research Institute (GCCRI) is a unique specialized cancer research center focusing on basic and translational research in childhood cancer and occupies a state-of-the-art 100,000 sq. foot research facility on the university’s Greehey Academic and Research Campus. The GCCRI has significant external funding, a large endowment from the state, and links to philanthropic entities. San Antonio is the nation’s seventh-largest city and is located at the edge of the beautiful Texas Hill County. San Antonio offers a rich, multi-cultural community, affordable cost of living, excellent weather, and a thriving biomedical industry. Interested candidates can submit their application (CV, a brief description of their accomplishments, and contacts of 3 references) directly by email to me (Email: gup...@uthscsa.edu) Thanks for your time. Yogesh -- Yogesh Gupta, PhD Assistant Professor of Biochemistry & Structural Biology PI, Greehey Children's Cancer Research Institute University of Texas Health Science Center 8403 Floyd Curl Drive, San Antonio, Texas, USA 78229 Email: gup...@uthscsa.edu http://ccri.uthscsa.edu/YGupta.html To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
[ccp4bb] Research Scientist position at UT Health San Antonio
My lab is looking for a Research Scientist. Interested candidates can directly apply via this link: https://uthscsa.referrals.selectminds.com/jobs/research-scientist-6174 Informal inquiries can be sent to me (Email: gup...@uthscsa.edu) *Job title: Research Scientist * *Job Summary:* The laboratory of Dr. Yogesh Gupta ( http://ccri.uthscsa.edu/YGupta.html) at Greehey Children’s Cancer Research Institute, the University of Texas Health at San Antonio, TX is seeking an enthusiastic research scientist to study protein-nucleic acid interactions in infectious diseases and pediatric cancers. *Job Duties: * 1. *Primary responsibility*: Large-scale expression and purification of recombinant proteins from insect and mammalian cells using conventional chromatography. 2. Biochemical and biophysical characterization of nucleic acid enzymes and their interaction with small molecules and nucleic acid substrates using isothermal titration calorimetry (ITC), surface plasmon resonance (SPR), and microscale thermophoresis (MST) techniques. 3. Maintain pediatric cancer cell lines and perform phenotypic screening in presence of new anti-cancer agents. Screen small molecule libraries for protein-drug interactions. 4. Assist PI in the preparation of research reports for presentations and publications. Work closely with the PI, other lab staff concerning scientific, research, and administrative matters. *Education: * - Ph.D. in biochemistry or structural biology, cell biology, cancer biology, with relevant experience in pathways and drug discovery. *Experience: * - Two (2) years of related experience is required. -- Yogesh Gupta, PhD Assistant Professor of Biochemistry & Structural Biology Greehey Children's Cancer Research Institute University of Texas Health Science Center at San Antonio 8403 Floyd Curl Drive, San Antonio, Texas, USA 78229 http://ccri.uthscsa.edu/YGupta.html To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
[ccp4bb] NIH funded postdoctoral position at University of Texas Health Science Center San Antonio
My research group at the University of Texas Health Science Center, San Antonio, is seeking to immediately hire a Postdoctoral Fellow to work on an NIH-funded project on SARS-CoV-2. We study the structures and mechanism of RNA modification and motor enzymes that operate during SARS-CoV-2 replication and transcription. We will use X-ray crystallography, cryo-EM, and enzyme biochemistry to address basic mechanistic questions and develop small-molecule antivirals. Further details of our work on SARS-CoV-2 can be found here: 1. Nature Communications. 2021 Jun 2; 12: 3287. PMID: 34078893. doi: 10.1038/s41467-021-23594-y <https://www.nature.com/articles/s41467-021-23594-y> 2. Nature Communications. 2020 Jul 24;11(1):3718. PMID: 32709886. doi: 10.1038/s41467-020-17496-8 <https://www.nature.com/articles/s41467-020-17496-8> Candidates with demonstrated experience in X-ray crystallography and a strong interest in using cryo-EM will be preferred. Candidates with research experience in cryo-EM are especially encouraged to apply. We already have crystals and cryo-EM datasets available for an immediate start. Interested candidates can submit their application (CV and a brief description of their accomplishments) directly by email: gup...@uthscsa.edu For detailed information about this position, please use this link: https://gccri.uthscsa.edu/job/postdoctoral-fellow-gupta-lab/ Thanks for your time. Yogesh -- Yogesh Gupta, PhD Assistant Professor of Biochemistry & Structural Biology Greehey Children's Cancer Research Institute University of Texas Health Science Center 8403 Floyd Curl Drive, San Antonio, Texas, USA 78229 http://ccri.uthscsa.edu/YGupta.html To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
[ccp4bb] Research Scientist and Postdoctoral Fellow positions available at University of Texas Health Science Center San Antonio
My research group at the University of Texas Health Science Center, San Antonio, is seeking to hire a Research Scientist and a Postdoctoral Fellow to study the structures and function of RNA modification and chromatin remodeling enzymes. We will use X-ray crystallography, cryo-EM, and enzyme biochemistry to address basic mechanistic questions and develop small-molecule antagonists. Candidates applying for the Research Scientist position must submit their application using the university's job portal: https://uthscsa.referrals.selectminds.com/jobs/research-scientist-5175 Candidates applying for the Postdoctoral position can submit their application (CV and brief description of their accomplishments) directly by email: gup...@uthscsa.edu For detailed information about this position, please use this link: https://gccri.uthscsa.edu/job/postdoctoral-fellow-gupta-lab/ Thank you for your time. Yogesh -- Yogesh Gupta, PhD Assistant Professor of Biochemistry & Structural Biology Greehey Children's Cancer Research Institute University of Texas Health Science Center 8403 Floyd Curl Drive, San Antonio, Texas, USA 78229 http://ccri.uthscsa.edu/YGupta.html To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
[ccp4bb] Postdoc position in San Antonio, TX
Dear Colleagues: My laboratory at the University of Texas Health Science Center at San Antonio is seeking an enthusiastic Postdoctoral Fellow to work on an NIH-funded project on SARS-CoV-2 and RNA modification. We will use X-ray crystallography, cryo-EM, and cell-based methods to address basic mechanistic questions and develop novel antivirals. These two papers describe our most recent work on SARS-CoV-2. Nature Communications. 2021 Jun 2; 12: 3287. PMID: 34078893. DOI: 10.1038/s41467-021-23594-y <https://www.nature.com/articles/s41467-021-23594-y> Nature Communications. 2020 Jul 24;11(1):3718. PMID: 32709886. DOI: 10.1038/s41467-020-17496-8 <https://www.nature.com/articles/s41467-020-17496-8> Preference will be given to applicants with demonstrated experience in purification and structural characterization of protein-nucleic acid complexes by X-ray crystallography with a strong interest in learning cryo-EM. An ideal candidate should hold (or soon expect to hold) a Ph.D. or equivalent degree in biochemistry/biophysics/structural biology and have no more than 3 years of postdoc experience. Salary is competitive and commensurate with experience. San Antonio is the nation’s seventh-largest city and is located at the edge of the beautiful Texas Hill County. San Antonio offers a rich, multi-cultural community, affordable cost of living, excellent weather, and a thriving biomedical industry. There is no state income tax in Texas. All Postdoctoral appointments are designated as security-sensitive positions. The University of Texas Health Science Center at San Antonio is an Equal Opportunity/Affirmative Action Employer, including protected veterans and persons with disabilities. If interested in applying, please send a short description of your research accomplishments and a CV with contact information of 3 references to Dr. Yogesh Gupta (email: gup...@uthscsa.edu). Yogesh -- Yogesh Gupta, PhD Assistant Professor of Biochemistry & Structural Biology Greehey Children's Cancer Research Institute University of Texas Health Science Center 8403 Floyd Curl Drive, San Antonio, TX, USA 78229 http://ccri.uthscsa.edu/YGupta.html To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
[ccp4bb] Postdoctoral position at UT Health San Antonio Texas
The laboratory of Dr. Yogesh Gupta (http://ccri.uthscsa.edu/YGupta.html) at the University of Texas Health at San Antonio (UTHSA) is seeking an enthusiastic Postdoctoral Fellow to study structures and function of RNA modification and chromatin remodeling complexes. We use structural biology (X-ray, NMR, cryo-EM) and cell-based methods to address basic mechanistic questions in viral pathogenesis and pediatric cancers. Further details of our most recent work can be found here: 1. Nature Communications. 2021 Jun 2; 12: 3287. PMID: 34078893. doi: 10.1038/s41467-021-23594-y <https://www.nature.com/articles/s41467-021-23594-y> 2. Nature Communications. 2020 Jul 24;11(1):3718. PMID: 32709886. doi: 10.1038/s41467-020-17496-8 <https://www.nature.com/articles/s41467-020-17496-8> Preference will be given to applicants with demonstrated experience in purification and characterization of protein-nucleic acid complexes by X-ray crystallography and strong interest in learning cryo-EM. Candidates are expected to possess strong organizational, interpersonal skills, and ability to work independently but also effectively and collaboratively as part of a team. An ideal candidate should hold (or soon expect to hold) a Ph.D. or equivalent degree in a related discipline and have no more than 2 years of postdoc experience. Our laboratory is located at Greehey Children’s Cancer Research Institute (GCCRI) of UTHSA. The GCCRI is a unique specialized cancer research center focusing on basic and translational research in childhood cancer and occupies a state-of-the-art 100,000 sq. foot research facility. The GCCRI has significant external funding, a large endowment from the state, and links to philanthropic entities. San Antonio is the nation’s seventh largest city and is located at the edge of the beautiful Texas Hill County. San Antonio offers a rich, multi-cultural community, affordable cost of living, excellent weather and a thriving biomedical industry. There is no state income tax in Texas. All Postdoctoral appointments are designated as security sensitive positions. The University of Texas Health Science Center at San Antonio is an Equal Opportunity/Affirmative Action Employer including protected veterans and persons with disabilities. Salary is competitive and commensurate with experience. If interested in applying, please send a short description of your research accomplishments and a CV with contact information of 3 references to Dr. Yogesh Gupta (email: gup...@uthscsa.edu). Yogesh -- Yogesh Gupta, PhD Assistant Professor of Biochemistry & Structural Biology PI, Greehey Children's Cancer Research Institute University of Texas Health Science Center 8403 Floyd Curl Drive, San Antonio, Texas, USA 78229 http://ccri.uthscsa.edu/YGupta.html To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
[ccp4bb] Postdoc position at University of Texas Health, San Antonio, TX, USA
*Postdoctoral Fellow position at UT Health School of Medicine, San Antonio, Texas* The laboratory of Dr. Yogesh Gupta (http://ccri.uthscsa.edu/YGupta.html) at the University of Texas Health at San Antonio is seeking an enthusiastic Postdoctoral Fellow to study structures and function of RNA modification and chromatin remodeling complexes. We use structural biology (X-ray, NMR, cryo-EM) and cell-based methods to address basic mechanistic questions in viral pathogenesis and pediatric cancers. The laboratory provides a rich multi-disciplinary training environment and access to cutting-edge technologies in structural biology and drug development. Further details of our most recent work can be found here: 1. Nature Communications. 2021 Jun 2; 12: 3287. PMID: 34078893. doi: 10.1038/s41467-021-23594-y <https://www.nature.com/articles/s41467-021-23594-y> 2. Nature Communications. 2020 Jul 24;11(1):3718. PMID: 32709886. doi: 10.1038/s41467-020-17496-8 <https://www.nature.com/articles/s41467-020-17496-8> Preference will be given to applicants with demonstrated experience in protein purification from insect/mammalian cells and structural characterization of protein-nucleic acid complexes by X-ray crystallography or cryo-EM. Candidates are expected to possess strong organizational, interpersonal skills, and ability to work independently but also effectively and collaboratively as part of a team. An ideal candidate should hold (or soon expect to hold) a Ph.D. or equivalent degree in a related discipline and have no more than 2 years of postdoc experience. The Greehey Children’s Cancer Research Institute (GCCRI) is a unique specialized cancer research center focusing on basic and translational research in childhood cancer and occupies a state-of-the-art 100,000 sq. foot research facility on the university’s Greehey Academic and Research Campus. The GCCRI has significant external funding, a large endowment from the state, and links to philanthropic entities. San Antonio is the nation’s seventh largest city and is located at the edge of the beautiful Texas Hill County. San Antonio offers a rich, multi-cultural community, affordable cost of living, excellent weather and a thriving biomedical industry. There is no state income tax in Texas. All Postdoctoral appointments are designated as security sensitive positions. The University of Texas Health Science Center at San Antonio is an Equal Opportunity/Affirmative Action Employer including protected veterans and persons with disabilities. Salary is competitive and commensurate with experience. If interested in applying, please send a short description of your research accomplishments and a CV with contact information of 3 references to Dr. Yogesh Gupta (email: gup...@uthscsa.edu). Yogesh -- Yogesh Gupta, PhD Assistant Professor of Biochemistry & Structural Biology PI, Greehey Children's Cancer Research Institute University of Texas Health Science Center 8403 Floyd Curl Drive, San Antonio, Texas, USA 78229 http://ccri.uthscsa.edu/YGupta.html To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
[ccp4bb] Postdoc position at UT Health Science Center, San Antonio, Texas
The laboratory of Dr. Yogesh Gupta at University of Texas Health Science Center in San Antonio is seeking an enthusiastic Postdoctoral Fellow to study the structure and function of nucleoproteins in infectious diseases and cancer. We combine structural biology tools (X-ray, cryo-EM, NMR) with cell-based methods to address mechanistic questions and exploit this knowledge for drug development. Please visit below websites to learn about our research program – https://www.nature.com/articles/s41467-020-17496-8 *http://ccri.uthscsa.edu/YGupta.html <http://ccri.uthscsa.edu/YGupta.html>* Preference will be given to applicants with demonstrated experience in macromolecular crystallography. Candidates are expected to possess strong organizational, interpersonal skills, and ability to work as part of a team. An ideal candidate should hold (or soon expect to hold) a Ph.D. or equivalent degree in biochemistry/biophysics/structural biology and have no more than 2 years of postdoc experience. Salary is competitive. San Antonio offers affordable cost of living and there is no state income tax in Texas. If interested in applying, please send a short description of your research accomplishments, and CV with a list of 3 references to Dr. Yogesh Gupta (email: *gup...@uthscsa.edu *). The Greehey Children’s Cancer Research Institute is a unique specialized cancer research center focusing on basic and translational research in childhood cancer and occupies a state-of-the-art 100,000 sq. foot research facility on the university’s Greehey Academic and Research Campus. San Antonio is the nation’s seventh largest city and is located at the edge of the beautiful Texas Hill Country. San Antonio offers a rich, multi-cultural community, affordable cost of living, excellent weather and a thriving biomedical industry.* All Postdoctoral appointments are designated as security sensitive positions. The University of Texas Health Science Center at San Antonio is an Equal Opportunity/Affirmative Action Employer including protected veterans and persons with disabilities.* Thank you very much for your time. Yogesh -- Yogesh Gupta, PhD Assistant Professor of Biochemistry & Structural Biology PI, Greehey Children's Cancer Research Institute University of Texas Health Science Center 8403 Floyd Curl Drive, San Antonio, Texas, USA 78229 http://ccri.uthscsa.edu/YGupta.html To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
[ccp4bb] Postdoctoral Fellow Position at University of Texas Health Science Center San Antonio
The laboratory of Dr. Yogesh Gupta at University of Texas Health Science Center in San Antonio (UT Health San Antonio™) is seeking a highly motivated and enthusiastic Postdoctoral Fellow to study protein-nucleic acid interactions in post-transcriptional gene regulation and chromatin biology. We combine various structural biology tools (X-ray, cryo-EM, NMR) with *in silico*, and cell-based methods to address basic mechanistic questions, and exploit this knowledge for drug development. Our well equipped lab is physically located within the Greehey Children’s Cancer Research Institute <http://gccri.uthscsa.edu/> (Greehey CCRI) at UT Health San Antonio. In addition, we have full access to an extensive array of institutional core facilities (Biochemistry core <http://biochem.uthscsa.edu/core_facilities.php>, CIDD <http://utcidd.org/>, Research core labs <http://research.uthscsa.edu/RCL/index.shtml>), and regular access to synchrotron beamlines. Applicants with demonstrated experience in protein biochemistry and structural biology, and keen interest in studying large protein-nucleic acid assemblies by X-ray and cryo-EM are preferred. Candidates are expected to possess strong organizational, interpersonal skills, and ability to work as part of a team. Candidate should hold (or soon expect to hold) a Ph.D. or equivalent degree in a related discipline. Prior experience in X-ray crystallography, biophysical methods (SPR, AUC, ITC, MST etc.), and enzyme kinetics is desirable. Salary is competitive ($52,704 per year plus standard fringe benefits of UT Health San Antonio). San Antonio offers affordable cost of living and there is no state income tax in Texas. Please visit the websites below to learn more about our work and future directions – http://ccri.uthscsa.edu/YGupta.html https://lsom.uthscsa.edu/biochemistry/team-member/yogesh-gupta-phd/ If interested in applying, please send a short description of your research accomplishments, and current CV with a list of three (3) references to Dr. Yogesh Gupta (email: gup...@uthscsa.edu). The Greehey Children’s Cancer Research Institute is a unique specialized cancer research center focusing on basic and translational research in childhood cancer and occupies a state-of-the-art 100,000 sq. foot research facility on the university’s Greehey Academic and Research Campus. San Antonio is the nation’s seventh largest city and is located at the edge of the beautiful Texas Hill County. San Antonio offers a rich, multi-cultural community, affordable cost of living, excellent weather and a thriving biomedical industry. *All Postdoctoral appointments are designated as security sensitive positions. The University of Texas Health Science Center at San Antonio is an Equal Opportunity/Affirmative Action Employer including protected veterans and persons with disabilities.* Thanks for you time. Yogesh -- Yogesh Gupta, PhD Assistant Professor of Biochemistry & Structural Biology PI, Greehey Children's Cancer Research Institute University of Texas Health Science Center 8403 Floyd Curl Drive, San Antonio, Texas, USA 78229 http://ccri.uthscsa.edu/YGupta.html email: gup...@uthscsa.edu phone: +1-210-562-9064 To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
[ccp4bb] Postdoc position in San Antonio, Texas
*Postdoctoral Fellow position at UT Health San Antonio, San Antonio, Texas* The laboratory of Dr. Yogesh Gupta (http://ccri.uthscsa.edu/YGupta.html) at University of Texas Health Science Center in San Antonio (UT Health San Antonio™) is seeking a motivated scientist with strong interest in structural/mechanistic studies on protein-nucleic acid assemblies in pediatric cancers. We combine structural/biophyiscal (X-ray, NMR, cryo-EM, AUC, SAXS), and cell-based methods to address basic mechanistic questions, and exploit this knowledge for drug discovery. Our lab is physically located within the Greehey Children’s Cancer Research Institute <http://gccri.uthscsa.edu/> (Greehey CCRI) at UT Health San Antonio. We have full access to an extensive array of core institutional facilities (Biochemistry core <http://biochem.uthscsa.edu/core_facilities.php>, CIDD <http://utcidd.org/>, Research core labs <http://research.uthscsa.edu/RCL/index.shtml>) located within the Department of Biochemistry and Structural Biology, the Joe R. and Teresa Lozano Long School of Medicine, and Greehey CCRI. Applicants with demonstrated experience in protein biochemistry, enzyme kinetics, and structural biology are preferred. Candidates are expected to possess strong organizational, interpersonal skills, and ability to work as part of a team. Candidate should hold (or soon expect to hold) a PhD or equivalent degree in a related discipline. An ideal candidate should not have >2 years of prior postdoc experience. Prior experience in X-ray crystallography is desirable but not required. Salary is competitive. San Antonio offers affordable cost of living and there is no state income tax in Texas. Please visit the websites below to learn more about our work and future directions – http://ccri.uthscsa.edu/YGupta.html If interested in applying, please send a short description of your research accomplishments, current CV with a list of three (3) references to Dr. Yogesh Gupta (email: gup...@uthscsa.edu). The Greehey Children’s Cancer Research Institute is a unique specialized cancer research center focusing on basic and translational research in childhood cancer and occupies a state-of-the-art 100,000 sq. foot research facility on the university’s Greehey Academic and Research Campus. San Antonio is the nation’s seventh largest city and is located at the edge of the beautiful Texas Hill County. San Antonio offers a rich, multi-cultural community, affordable cost of living, excellent weather and a thriving biomedical industry. *All Postdoctoral appointments are designated as security sensitive positions. The University of Texas Health Science Center at San Antonio is an Equal Opportunity/Affirmative Action Employer including protected veterans and persons with disabilities.* -- Yogesh Gupta, PhD Assistant Professor of Biochemistry & Structural Biology PI, Greehey Children's Cancer Research Institute University of Texas Health Science Center 8403 Floyd Curl Drive, San Antonio, Texas, USA 78229 http://ccri.uthscsa.edu/YGupta.html To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
[ccp4bb] Postdoc position at UT Health Science Center at San Antonio, Texas
The laboratory of Dr. Yogesh Gupta at University of Texas Health Science Center in San Antonio (UT Health San Antonio™) is seeking a highly motivated and enthusiastic Postdoctoral Fellow with strong interest in structural and mechanistic studies on protein-nucleic acid complexes implicated in post-transcriptional gene regulation and chromatin biology. We combine various structural biology tools (X-ray, NMR, cryo-EM, AUC, SAXS) with *in silico*, and cell-based methods to address basic mechanistic questions, and exploit this knowledge for drug discovery. Our well equipped lab is physically located within the Greehey Children’s Cancer Research Institute <http://gccri.uthscsa.edu/> (Greehey CCRI) at UT Health San Antonio. In addition, we have full access to an extensive array of institutional core facilities (Biochemistry core <http://biochem.uthscsa.edu/core_facilities.php>, CIDD <http://utcidd.org/>, Research core labs <http://research.uthscsa.edu/RCL/index.shtml>), and regular access to synchrotron beamlines. Applicants with demonstrated experience in protein biochemistry and structural biology, and a keen interest in studying large macro-molecular assemblies are preferred. Candidates are expected to possess strong organizational, interpersonal skills, and ability to work as part of a team. Candidate should hold (or soon expect to hold) a PhD or equivalent degree in a related discipline. Prior experience in X-ray crystallography, biophysical methods (SPR, AUC, ITC etc.), and enzyme kinetics is desirable. Salary is competitive. San Antonio offers affordable cost of living and there is no state income tax in Texas. Please visit the websites below to learn more about our work and future directions – http://ccri.uthscsa.edu/YGupta.html If interested in applying, please send a short description of your research accomplishments, and current CV with a list of three (3) references to Dr. Yogesh Gupta (email: gup...@uthscsa.edu). The Greehey Children’s Cancer Research Institute is a unique specialized cancer research center focusing on basic and translational research in childhood cancer and occupies a state-of-the-art 100,000 sq. foot research facility on the university’s Greehey Academic and Research Campus. San Antonio is the nation’s seventh largest city and is located at the edge of the beautiful Texas Hill County. San Antonio offers a rich, multi-cultural community, affordable cost of living, excellent weather and a thriving biomedical industry. *All Postdoctoral appointments are designated as security sensitive positions. The University of Texas Health Science Center at San Antonio is an Equal Opportunity/Affirmative Action Employer including protected veterans and persons with disabilities.* -- Yogesh Gupta, PhD Assistant Professor of Biochemistry & Structural Biology PI, Greehey Children's Cancer Research Institute University of Texas Health Science Center 8403 Floyd Curl Drive, San Antonio, Texas, USA 78229 http://ccri.uthscsa.edu/YGupta.html Tel: +1-210-562-9064 To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
[ccp4bb] Research Scientist position at University of Texas Health Science Center at San Antonio, Texas
*Research Scientist position at UT Health Science Center, San Antonio, TX* *Job Summary: * The laboratory of Dr. Yogesh Gupta (http://ccri.uthscsa.edu/YGupta.html) at Greehey Children’s Cancer Research Institute, University of Texas Health at San Antonio, TX is seeking an enthusiastic scientist to study structure and function of protein-nucleic acid complexes. *Job Duties: * 1. Express human proteins with relevance to cancer from bacterial, insect, and mammalian cells. Scale up the culture volume to produce these potential drug targets and perform their biochemical characterization by various cell enzymatic assays. Perform co-crystallization and determine the structures of protein-nucleic acid complexes using X-ray crystallography and other biophysical methods. 2. Develop, design, and perform cancer cell biology experiments using cell imaging, tissue culture, FACS, mice, Cas/CRISPR knock out methods to characterize new signaling pathways. Perform data analysis and interpret results. Review progress and present results regularly to PI. 3. Assist PI in preparation of research reports, manuscripts, papers and grant proposals for presentation and publication. Research current scientific literature for novel or improved research protocols; work closely with the PI, other lab staff concerning scientific, research and administrative matters *Preferred Qualifications:* Ph.D. in cell biology, cancer biology, biochemistry/structural biology with relevant experience in cancer cell models, pathways and drug discovery. Salary is competitive. San Antonio offers affordable cost of living and there is no state income tax in Texas. *Submit your application to* *https://uthscsa.referrals.selectminds.com/jobs/research-scientist-600 <https://uthscsa.referrals.selectminds.com/jobs/research-scientist-600>* The Greehey Children’s Cancer Research Institute is a unique specialized cancer research center focusing on basic and translational research in childhood cancer and occupies a state-of-the-art 100,000 sq. foot research facility on the university’s Greehey Academic and Research Campus. San Antonio is the nation’s seventh largest city and is located at the edge of the beautiful Texas Hill County. San Antonio offers a rich, multi-cultural community, affordable cost of living, excellent weather and a thriving biomedical industry. *All Postdoctoral appointments are designated as security sensitive positions. The University of Texas Health Science Center at San Antonio is an Equal Opportunity/Affirmative Action Employer including protected veterans and persons with disabilities.* -- Yogesh Gupta, PhD Assistant Professor of Biochemistry & Structural Biology PI, Greehey Children's Cancer Research Institute University of Texas Health Science Center 8403 Floyd Curl Drive, San Antonio, Texas, USA 78229 http://ccri.uthscsa.edu/YGupta.html To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
[ccp4bb] Postdoc position at University of Texas Health Science Center at San Antonio
*Postdoctoral Fellow position at UT Health San Antonio, San Antonio, Texas* The laboratory of Dr. Yogesh Gupta (http://ccri.uthscsa.edu/YGupta.html) at University of Texas Health Science Center in San Antonio (UT Health San Antonio™) is seeking a highly motivated and enthusiastic scientist with strong interest in structural/mechanistic studies on protein-nucleic acid assemblies that play critical roles in leukemia and pediatric sarcoma pathogenesis. We combine various structural and chemical biology tools (X-ray, NMR, cryo-EM, AUC, SAXS) with *in silico*, and cell-based methods to address basic mechanistic questions, and exploit this knowledge for small molecule cancer drug discovery. Our lab is physically located within the Greehey Children’s Cancer Research Institute <http://gccri.uthscsa.edu/> (Greehey CCRI) at UT Health San Antonio. We have full access to an extensive array of core institutional facilities (Biochemistry core <http://biochem.uthscsa.edu/core_facilities.php>, CIDD <http://utcidd.org/>, Research core labs <http://research.uthscsa.edu/RCL/index.shtml>) located within the Department of Biochemistry and Structural Biology, the Joe R. and Teresa Lozano Long School of Medicine, and Greehey CCRI. Applicants with demonstrated experience in protein biochemistry, structural biology, or cancer biology with experience in cell imaging, tissue culture, FACS, mice, Cas/CRISPR knock out, and a keen interest in mechanistic/structural studies are preferred. Candidates are expected to possess strong organizational, interpersonal skills, and ability to work as part of a team. Candidate should hold (or soon expect to hold) a PhD or equivalent degree in a related discipline. Prior experience in X-ray crystallography is desirable but not required. Salary is competitive. San Antonio offers affordable cost of living and there is no state income tax in Texas. Please visit the websites below to learn more about our work and future directions – http://ccri.uthscsa.edu/YGupta.html If interested in applying, please send a short description of your research accomplishments, and current CV with a list of three (3) references to Dr. Yogesh Gupta (email: gup...@uthscsa.edu). The Greehey Children’s Cancer Research Institute is a unique specialized cancer research center focusing on basic and translational research in childhood cancer and occupies a state-of-the-art 100,000 sq. foot research facility on the university’s Greehey Academic and Research Campus. San Antonio is the nation’s seventh largest city and is located at the edge of the beautiful Texas Hill County. San Antonio offers a rich, multi-cultural community, affordable cost of living, excellent weather and a thriving biomedical industry. *All Postdoctoral appointments are designated as security sensitive positions. The University of Texas Health Science Center at San Antonio is an Equal Opportunity/Affirmative Action Employer including protected veterans and persons with disabilities.* -- Yogesh Gupta, PhD Assistant Professor of Biochemistry & Structural Biology PI, Greehey Children's Cancer Research Institute University of Texas Health Science Center 8403 Floyd Curl Drive, San Antonio, Texas, USA 78229 http://ccri.uthscsa.edu/YGupta.html To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
[ccp4bb] Research Scientist position at UT Health Science Center, San Antonio, Texas
A *Research Scientist* position is available immediately in my lab at University of Texas Health Science Center San Antonio to pursue mechanistic and structural studies on protein-nucleic acid complexes in pediatric cancers. The successful applicant should hold a PhD, have a strong background in cancer cell biology and have a keen interest in structure-based studies. Here are a few links: 1. More details and instructions for applying - https://www.uthscsajobs.com/postings/14293 2. Our group – http://ccri.uthscsa.edu/YGupta.html Best, Yogesh -- Yogesh Gupta, PhD Assistant Professor of Biochemistry & Structural Biology PI, Greehey Children's Cancer Research Institute University of Texas Health Science Center 8403 Floyd Curl Drive, San Antonio, Texas, USA 78229 http://ccri.uthscsa.edu/YGupta.html To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
[ccp4bb] Postdoctoral position in RNA Epigenetics at UT Health San Antonio, Texas
*Postdoctoral Fellow position in RNA Epigenetics at UT Health San Antonio, San Antonio, Texas* The laboratory of Dr. Yogesh Gupta (http://ccri.uthscsa.edu/YGupta.html) at The University of Texas Health Science Center in San Antonio (UT Health San Antonio™) is seeking a highly motivated and enthusiastic scientist with a strong interest in mechanistic studies on large protein-nucleic acid assemblies that play critical roles in leukemia and pediatric sarcoma pathogenesis. We are particularly interested in understanding the exact mechanisms by which different enzymes and accessory factors cross talk, assemble, and dynamically regulate the covalent chemical modifications on both coding and non-coding RNA transcripts. We employ a powerful combination of structural and chemical biology tools with an array of other biophysical, *in silico*, and cell-based methods to address prevailing questions for the emerging field of RNA epigenetics. Our lab is physically located within the Greehey Children’s Cancer Research Institute <http://gccri.uthscsa.edu/> (Greehey CCRI) at UT Health San Antonio. We have full access to an extensive array of core institutional facilities (Biochemistry core <http://biochem.uthscsa.edu/core_facilities.php>, CIDD <http://utcidd.org/>, Research core labs <http://research.uthscsa.edu/RCL/index.shtml>) located within the Department of Biochemistry and Structural Biology, the Joe R. and Teresa Lozano Long School of Medicine, and Greehey CCRI. Applicants with demonstrated experience in protein biochemistry, sarcoma or leukaemia biology with experience in cell imaging, tissue culture, FACS, mice, Cas/CRISPR knock out, and a keen interest in mechanistic/structural studies are preferred. Candidates are expected to possess strong organizational, interpersonal skills, and ability to work as part of a team. Candidate should hold (or soon expected to hold) a PhD or equivalent degree in a related discipline. Prior experience in X-ray crystallography or NMR is not required. Please visit the websites below to learn more about our work and future directions – http://ccri.uthscsa.edu/YGupta.html If interested in applying, please send a short description of your research accomplishments, and current CV with a list of three (3) references to Dr. Yogesh Gupta (email: gup...@uthscsa.edu). The Greehey Children’s Cancer Research Institute is a unique specialized cancer research center focusing on basic and translational research in childhood cancer, and occupies a state-of-the-art 100,000 sq. foot research facility on the university’s Greehey Academic and Research Campus. San Antonio is the nation’s seventh largest city and is located at the edge of the beautiful Texas Hill County. San Antonio offers a rich, multi-cultural community, affordable cost of living, excellent weather and a thriving biomedical industry. *All Postdoctoral appointments are designated as security sensitive positions. The University of Texas Health Science Center at San Antonio is an Equal Opportunity/Affirmative Action Employer including protected veterans and persons with disabilities.* -- Yogesh Gupta, PhD Assistant Professor of Biochemistry & Structural Biology PI, Greehey Children's Cancer Research Institute University of Texas Health Science Center 8403 Floyd Curl Drive, San Antonio, Texas, USA 78229 http://ccri.uthscsa.edu/YGupta.html To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
[ccp4bb] Postdoctoral position in Structural Biology of RNA Methylation Complexes, UTHSC at San Antonio, Texas
*Postdoctoral Fellow position at The University of Texas Health Science Center at San Antonio (San Antonio, Texas)* The laboratory of Yogesh Gupta (www.guptalabgccri.org) at The University of Texas Health Science Center in San Antonio (UT Health San Antonio™) is seeking a highly motivated and enthusiastic scientist with a strong interest in structural and mechanistic studies on large protein-nucleic acid complexes that play critical roles in childhood cancers. We are particularly interested in understanding the exact mechanisms by which different enzymes and accessory factors cross talk, assemble, and dynamically regulate the covalent chemical modifications on both coding and non-coding RNA transcripts. We employ a powerful combination of structural biology tools (X-ray crystallography, NMR, AUC) with an array of other biophysical, *in silico*, and cell based methods to address prevailing questions on this emerging field of RNA epigenetics. Our lab is physically located within the Greehey Children’s Cancer Research Institute <http://gccri.uthscsa.edu/> (Greehey CCRI) at UT Health San Antonio. We have regular access to an extensive array of core institutional facilities (Biochemistry core <http://biochem.uthscsa.edu/core_facilities.php>, CIDD <http://utcidd.org/>, Research core labs <http://research.uthscsa.edu/RCL/index.shtml>) located within the Department of Biochemistry and Structural Biology, the Joe R. and Teresa Lozano Long School of Medicine, and Greehey CCRI. Applicants with demonstrated experience in protein biochemistry, molecular and structural biology, mammalian cell culture and a keen interest in structures of large macromolecular complexes are preferred. Candidates are expected to possess strong organizational, interpersonal skills, and ability to work as part of a team. Candidate should hold (or soon expected to hold) a PhD or equivalent degree in a related discipline. Prior experience in X-ray crystallography is desired but not required. To learn more about our work and future directions, please visit the website - http://www.guptalabgccri.org <http://www.guptalabgccri.org/> If interested in applying, please send a short description of your research accomplishments, and current CV with a list of three references to Dr. Yogesh Gupta (email: gup...@uthscsa.edu). The Greehey Children’s Cancer Research Institute is a unique specialized cancer research center focusing on basic and translational research in childhood cancer, and occupies a state-of-the-art 100,000 sq. foot research facility on the university’s Greehey Academic and Research Campus. San Antonio is the nation’s seventh largest city and offers a rich, multi-cultural community with a thriving bioscience industry. It is located at the edge of the beautiful Texas Hill Country. *All Postdoctoral appointments are designated as security sensitive positions. The University of Texas Health Science Center at San Antonio is an Equal Opportunity/Affirmative Action Employer including protected veterans and persons with disabilities.* -- Yogesh Gupta, PhD Assistant Professor Greehey Children's Cancer Research Institute & Dept. of Biochemistry & Structural Biology University of Texas Health Science Center 8403 Floyd Curl Drive, San Antonio, Texas, USA 78023 phone: 210-562-9064 email: gup...@uthscsa.edu http://www.guptalabgccri.org/
[ccp4bb] Cys auxotroph
Does anyone know a commercial source of Cys-auxotroph strain of E. coli to prepare a doubly (Se-Cys + Se-Met) labeled protein? I would appreciate if you can share your experience if you have labeled your protein in this way to improve Se signal for phasing. Thanks, -Yogesh -- -- Yogesh K. Gupta, Ph.D. Dept of Structural Chemical Biology Mount Sinai School of Medicine Icahn Medical Institute 1 Gustave L. Levy Place, Box 1677 New York, NY, USA 10029 Tel:+1 212-659-8639 --
[ccp4bb] f2mtz error: problems reading reflection
Dear Experts, After a new installation on Mac OS 10.6, i am getting this error (related to f2mtz) during the Find SITES step by SHELX in Autosharp. Data line--- LABO H K L FA SIGFA ALPHA Number of columns to be read in: 6 Data line--- CTYP H H H F Q P Data line--- FORM '(3F4.0,2F8.2,F4.0)' $TEXT:Warning: $$ comment $$ WARNING: Format I replaced with F $$ FORMATTED OLD file opened on unit 1 Logical name: HKLIN, Filename: SHELX/1_fa.hkl $TEXT:Warning: $$ comment $$ WARNING: PROJECTNAME not assigned $$ $TEXT:Warning: $$ comment $$ WARNING: CRYSTALNAME not assigned $$ $TEXT:Warning: $$ comment $$ WARNING: DATASETNAME not assigned $$ *** Read error Check FORMAT -- especially the need for REALs f2mtz: problems reading reflection 9 f2mtz: problems reading reflection 9 I am running ccp4-6.1.13. It looks like a FORTRAN error but do not know how to fix this or which file to edit particularly. SHARP otherwise running fine if i exclude SHELX step by feeding known sites. Thanks for your help. Yogesh -- Yogesh K. Gupta, Ph.D. Dept of Structural Chemical Biology Mount Sinai School of Medicine Icahn Medical Institute 1425, Madison Av. (Rm 16-26) New York, NY 10029 Tel:+1 212-659-8639 -
[ccp4bb] column labels for SIRAS/MIRAS
Hello, My aim is to calculate SIRAS/MIRAS phases using PHENIX. Which should be the correct column labels and associated Differences in the derivative data one should select to calculate SIRAS phases? I am supplying both native and derivative data and for the derivative data i am selecting F_HA, SigF_HA, DANO_HA, SigDANO_HA as labels and 'Anomalous' as differences. Should the differences not be 'Isomorphous+Anomalous' in case of SIRAS? If so, which column label is the correct one if i choose Iso+Ano as differences? Running SIRAS in SHARP requires Fs,DANOs, and ISYM for the derivative. It seems that columns F_HA(+),SigF_HA(+),F_HA(-), and SigF(-) can also be used for SIRAS as mentioned below, which confused me a little bit. http://www.ccp4.ac.uk/HAPPy/docshow.php?doc=design/input.html#input Could someone please clarify? Thanks for your time and help. Yogesh
Re: [ccp4bb] pH-ing Jeffamine ED-2001
Hi Amit, Changing the pH of Jeffamine is not so easy . I would keep the pH of Jeffamine constant and rather change the pH of buffer. Anyway if Jeffamine concentration is too low as compare to the buffer you are using, it would not change the final pH much. This is what my experience with Jeffamine is. Cheers, Yogesh On Mon, Apr 21, 2008 at 9:20 AM, amit sharma [EMAIL PROTECTED] wrote: Dear CCP4ers, Apologies for a non-CCP4 query. I intend to set up an optimization grid around one of the crystallization conditions (containing Jeffamine ED-2001pH 7.0 and HEPES pH 7.0) by varying the buffer pH from 5.5 to 7.5. In such a case, is it recommmended that the pH of Jeffamine ED-2001(pH 7.0) also be changed to match the different buffer pHs(5.5-7.5)? Cheers, Amit -- Yogesh K. Gupta, Ph.D. Post Doctoral Fellow Molecular Physiology Biophysics Mount Sinai School of Medicine 1425, Madison Av. (East Bldg. 16-26) Box 1677, New York, NY 10029-6574 Tel:+1 212-659-8639 Fax:+1 212-849-2456 E-mail: [EMAIL PROTECTED] -
[ccp4bb] TLS refinement in REFMAC
Dear CCP4 users, I refined my final model (protein-RNA complex) using TLS refinement in Refmac but when i am validating the coordinates I am having an issue with Bond Distances and Bond Angles of some of the bases of RNA, these values for some of the bases are higher ( 6 times) then the standard values. I figured that it happened only after REFMAC refinement. The coordinates before the REFMAC (the last CNS refined coordinates) were found to be acceptable by the same validation program. So my guess is something wierd is happening with RNAs only during REFMAC refinement. Although, Rfree improved by almost 1.5% with REFMAC. In an attempt to fix this, I ommitted both chains of the RNAs during the REFMAC (.tls file contains only protein monomers) but that did not resolve the isssue either. Is there any way to fix this problem or a way to restrain particular RNA bases/chains during TLS refinement? Thanks for your time and help. Happy Holidays Yogesh -- Yogesh K. Gupta, Ph.D. Post Doctoral Fellow Molecular Physiology Biophysics Mount Sinai School of Medicine 1425, Madison Av. (East Bldg. 16-26) Box 1677, New York, NY 10029-6574 Tel:+1 212-659-8639 Fax:+1 212-849-2456 E-mail: [EMAIL PROTECTED] -
