Next MX-proposal application deadline: March 01, 2017 is approaching http://www.helmholtz-berlin.de/user/beamtime/proposals/index_en.html
Hereby we would like to invite the submission of new proposals for MX-beamtime at the HZB-MX beamlines for the next beam time period (08/2017-01/2018). In order to apply for beamtime, please register at the HZB on-line access tool "GATE" (https://www.helmholtz-berlin.de/pubbin/hzbgate) and submit a new beam time application proposal. What's new: In situ-screening in crystallization plates on BL14.1 will be reinstalled from March 2017 onwards! HZB provides MX-beamtime at the three MX-beamlines BL14.1, BL14.2 and BL14.3. The three beamlines are equipped with state-of-the-art instrumentation and are currently the most productive MX-stations in Germany with over 300 PDB depositions annually. Beamtime is granted based on the reviewed proposals and on reports from previous research activities. Please make sure to include them if available. Experimental setup: BL14.1: - Photon flux: 1.4x10¹¹ Phot/sx100mAx0.05%BW at sample position (0.1-1 sec exposure time per frame) - User defined beam shaping from 50µm-100µm diameter possible - Pilatus 6M detector, 141mm-680mm max. distance from the sample - Microdiffractometer (MD2) with Mini-kappa goniometer MK3 - Automatic sample changer (CATS), 90 sample storage capacity (SPINE-Pin & EMBL sample magazine compatibility) - 32-core XEON-CPU server, with 10Gb uplink to Pilatus 6M - Data collection control via MXCuBE - EDNA - Common MX-software installed including XDS, iMOSFLM, CCP4, Phenix, SHELXC-E, etc. - Automated data processing with XDSAPP - Remotely controlled cryo-shutter for crystal annealing - Pressure chamber for noble gas derivatization (Xe, Kr available upon request) - AMPTEK-XRF detector and XFEPLOT software available We are also offering the hard- and software environment for carrying out: - UV-RIP experiments at BL14.1. For further information, please visit: http://www.helmholtz-berlin.de/forschung/funkma/soft-matter/forschung/bessy-mx/ancillary-facilities/uvrip_en.html BL14.2: - Photon flux: 1.9x10¹¹ Phot/sx100mAx0.05%BW at sample position (0.1-1 sec exposure time per frame) - Pilatus 3S-2M detector with 1000 micron Si sensor thickness, 55mm-600mm distance from the sample - Nanodiffractometer (DESY P11 design) and on-axis sample microscope - User defined beam shaping from 30µm-150µm diameter possible - GROB sample changer for SPINE and UNIPUCK support - 60-core XEON-CPU server, with fibre channel SAN up-link data processing environment - Common MX-software installed including XDS, iMOSFLM, CCP4, Phenix, SHELXC-E, etc. - Automated data processing with XDSAPP - Amptek XRF detector - Pressure chamber for noble gas derivatization (Xe, Kr available upon request) - Ultra high performance stereo microscope Leica M205A, 20-255x zoom, 8 Mpixel CCD-camera - UV-Microsprectrophotometer offline setup available - AMPTEK-XRF detector and XFEPLOT software available BL14.3: - Photon flux: 4x10exp10 Phot/sx100mAx0.05%BW at sample position (3-20 sec exposure time per frame) - Rayonix MX-225 X-ray detector, 45mm-380mm distance from the sample, 30 deg 2-Theta possible - MARdtb goniometer - 60-core XEON-CPU server, with fibre channel SAN up-link data processing environment - EDNA installed and available - Common MX software installed including XDS, iMOSFLM, CCP4, Phenix, SHELXC-E, etc. - Automated data processing using XDSAPP - Remotely controlled cryo-shutter for crystal annealing - HC1c dehydration device installed (please specify HC1-beamtime in your proposal if needed) - Pressure chamber for noble gas derivatization (Xe, Kr available upon request) - Ultra high performance stereo microscope Leica M205A, 20-255x zoom, 8 Mpixel CCD-camera S1-biolab facilities (separate registration required): - Protein production and purification - Nanoliter 96 well crystallization plate formulation and storage at 5 °C and 20 °C - Biophysical characterization with real time PCR (thermofluor assay) The HZB-MX group is also providing expert assistance as well as access to a library of fragments for carrying out crystallographic fragment-screening experiments. For more information please contact us at mswe...@helmholtz-berlin.de. Please visit our web page www.helmholtz-berlin.de/bessy-mx to obtain updated information about our experimental setup and other requirements. Manfred Weiss and the HZB-MX group -- Dr. Manfred. S. Weiss Helmholtz-Zentrum Berlin für Materialien und Energie Macromolecular Crystallography (HZB-MX) Albert-Einstein-Str. 15 D-12489 Berlin GERMANY Fon: +49-30-806213149 Fax: +49-30-806214975 Web: http://www.helmholtz-berlin.de/bessy-mx Email: mswe...@helmholtz-berlin.de ________________________________ Helmholtz-Zentrum Berlin für Materialien und Energie GmbH Mitglied der Hermann von Helmholtz-Gemeinschaft Deutscher Forschungszentren e.V. Aufsichtsrat: Vorsitzender Dr. Karl Eugen Huthmacher, stv. Vorsitzende Dr. Jutta Koch-Unterseher Geschäftsführung: Prof. Dr. Anke Rita Kaysser-Pyzalla, Thomas Frederking Sitz Berlin, AG Charlottenburg, 89 HRB 5583 Postadresse: Hahn-Meitner-Platz 1 D-14109 Berlin http://www.helmholtz-berlin.de