Quite common. And it may be a good sign for crystallizability in those
conditions. As the concentration gradients dissipate from the initially
mixed drop, your protein re-solubilizes.
Roger Rowlett
On Aug 17, 2012 5:31 PM, "Yuri Pompeu" wrote:
> Hello everyone,
> I have just made a curious observation.
> I purified an enzyme domain and concentrated it to around 16 mg/mL without
> much mischief.
> I then proceeded to set-up some drops. After 5 min most of the drops had a
> muddy appearance to them which led me to think I was probably too
> concentrated in either protein and/or precipitant.
> Curiously I went back to look at the drops after an additional 30 min and
> they all look pretty clear with no appreciable precipitation.
> Has anyone encountered this situation or a similar one before?
> Any input/shared experience is welcome.
> Best,
> Yuri
>
