We have had some good luck with hydrogen peroxide [for technical details plus validation via a crystal structure see Van der Meeren et al JBC 288(2):1214-1225, (2013)]. best of luck Savvas
On 28 Feb 2013, at 12:14, "McEwan, Paul" <paul.mce...@evotec.com> wrote: > Rather than looking at “anti-DTT” it is more important to set up an > appropriate redox system. This can be a combination of reduced and oxidised > glutathione or cysteine. If you check some of the commercial protein > refolding screens this should give you an idea about relative concentrations. > > Best regards, > Paul.. > > ----------------------------------------------------------------------------------------- > Dr. Paul A. McEwan > Senior Scientist (Structural Biology) > Evotec (UK) Ltd. > 114 Innovation Drive | Milton Park | Abingdon | Oxfordshire | OX14 4SA > > email: paul.mce...@evotec.com > Tel: +44 (0)1235 861561 > Fax:+44 (0)1235 863139 > direct line: +44 (0)1235 838802 > www.evotec.com > > From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Jacob > Keller > Sent: 28 February 2013 11:09 > To: CCP4BB@JISCMAIL.AC.UK > Subject: Re: [ccp4bb] disulfide engineering > > Along these lines, what reagents do people use to promote disuflide bonds, > i.e., the "anti-DTT?" > > JPK > > On Thu, Feb 28, 2013 at 2:06 AM, David Briggs <drdavidcbri...@gmail.com> > wrote: > You might want to try "Disulfide by design" > > http://cptweb.cpt.wayne.edu/DbD2/ > > Cheers > > Dave > > On Feb 28, 2013 6:55 AM, "Careina Edgooms" <careinaedgo...@yahoo.com> wrote: > Dear CCP4 members > > I wish to engineer a disulfide bond at the dimer interface of a protein I am > working with. Does anyone know of any available software to assist with this? > > Best > Careina > > > > -- > ******************************************* > Jacob Pearson Keller, PhD > Postdoctoral Associate > HHMI Janelia Farms Research Campus > email: j-kell...@northwestern.edu > ******************************************* > Evotec (UK) Ltd is a limited company registered in England and Wales. > Registration number:2674265. Registered Office: 114 Milton Park, Abingdon, > Oxfordshire, OX14 4SA, United Kingdom >
