Re: [ccp4bb] Fabricating hamilton syringe coupler for LCP preparation
Hi Thomas We use a very simple solution. We simply shorten a normal needle to 6.5 mm and push it into another syringe that has *two *of the white PTFE ferrules made by Hamilton. (We salvage the extra ferrule from another needle.) We use a 22 gauge needle (not 22S, where the S stands for *small *inner diameter). Now you can push the two syringes together and make your LCP. It helps if you tighten the knurled nose-piece to compress the PTFE ferrules before you join the syringes. You have to keep a little pressure on the two syringes to hold them together while you mix up the LCP, or use eg tape. (Which is why we've made a little mixer on a rail, but that's another story.) One nice feature is that you hardly waste any LCP and the needle for dispensing it is already attached to the syringe. Let me know if you need more info or you'd like me to send you a short needle. Best wishes, Patrick On 16 December 2014 at 02:14, Thomas Cleveland thomas.clevel...@gmail.com wrote: Hi everyone, Thanks for the advice. I had, in fact, already ordered some commercial couplers, but they haven't come in yet, and there was an experiment I wanted to do today. Here's what I found: 1. The steel ferrules have an orientation, with a tight side and a loose side. They can be removed by sliding in one direction, but not the other. Even then, it's pretty difficult. I had to use pliers, and pieces of needle broke off during the process. The tight side of the ferrule then needed to be reamed open slightly with a steel tool before I was able to slide the ferrule onto the other needle. 2. Soldering stainless steel is really a pain. In the end I got a coupler that seems to work well, but it was a pain, and it's a bit charred looking. Thanks again, Tom On Mon, Dec 15, 2014 at 6:01 PM, Aaron Thompson aaron.a.thomp...@gmail.com wrote: I agree with Jim – purchasing the couplers will get you up and running much quicker. TTP also sells nice couplers: https://www.ttplabtechstore.com/ttp_ecom/cc/ItemDetails.jsp?@where.ItemID@EQ=3072-01050sessionkey=# Aaron On Mon, Dec 15, 2014 at 12:38 PM, Bernhard Rupp hofkristall...@gmail.com wrote: Tried the RN kludge at least I did not get it to work. You cannot tighten the plastic swage lock type sleeves tight enough. On operation the pressure drives the PEEK tubing out of the compression fit. Maybe if you have a jig that holds the 2 syringes in a fixed position so they cannot move apart it can work. Best, BR -Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Daniel Anderson Sent: Monday, December 15, 2014 8:48 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Fabricating hamilton syringe coupler for LCP preparation Here's my addition to Jim Fairman's reply: You could use a pair of RN compression fittings (www.hamilton dot com part number 55751-01) and a segment of HPLC tubing. HPLC tubing within my field of view can have an inside diameter as small as 0.005 inch. hope that helps, Happy Merry, etc., Dan On 12/15/2014 11:09 AM, Thomas Cleveland wrote: Hi all, I'm trying to put together some homemade syringe couplers following the published instructions from the Caffrey group. I'm having a bit of trouble with this part: The stainless steel ferrule of the second needle is removed and placed on the free end of the coupling needle such that the double thumb nut is held symmetrically between the two steel ferrules Has anyone done this, and if so, how did you remove the stainless steel ferrule from the second needle in order to place it over the first? The stainless steel ferrule appears to be firmly attached and I'm having trouble removing it. Thanks, Thomas Cleveland -- patr...@douglas.co.ukDouglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36
[ccp4bb] Fabricating hamilton syringe coupler for LCP preparation
Hi all, I'm trying to put together some homemade syringe couplers following the published instructions from the Caffrey group. I'm having a bit of trouble with this part: The stainless steel ferrule of the second needle is removed and placed on the free end of the coupling needle such that the double thumb nut is held symmetrically between the two steel ferrules Has anyone done this, and if so, how did you remove the stainless steel ferrule from the second needle in order to place it over the first? The stainless steel ferrule appears to be firmly attached and I'm having trouble removing it. Thanks, Thomas Cleveland
Re: [ccp4bb] Fabricating hamilton syringe coupler for LCP preparation
As a user of many of these coupling devices, I would highly suggest buying your couplers pre-fabricated and avoiding many hours (and possibly days) of headaches trying to manufacture these with your own hands. Rigaku Reagents sells them: https://www.rigakureagents.com/p-516-wizard-cubic-lcp-kit.aspx Formulatrix sells them a bit cheaper: http://formulatrix.com/store/product.php?productid=27cat=4page=1 I have used both products and they both function well even after many uses and cleaning cycles in methanol. Cheers, Jim On Mon, Dec 15, 2014 at 11:09 AM, Thomas Cleveland thomas.clevel...@gmail.com wrote: Hi all, I'm trying to put together some homemade syringe couplers following the published instructions from the Caffrey group. I'm having a bit of trouble with this part: The stainless steel ferrule of the second needle is removed and placed on the free end of the coupling needle such that the double thumb nut is held symmetrically between the two steel ferrules Has anyone done this, and if so, how did you remove the stainless steel ferrule from the second needle in order to place it over the first? The stainless steel ferrule appears to be firmly attached and I'm having trouble removing it. Thanks, Thomas Cleveland -- Jim Fairman, Ph D. Group Leader I - Crystallography Beryllium http://www.be4.com Tel: 206-780-8914 Cell: 240-479-6575 E-mail: fairman@gmail.com jfair...@embios.com
Re: [ccp4bb] Fabricating hamilton syringe coupler for LCP preparation
Here's my addition to Jim Fairman's reply: You could use a pair of RN compression fittings (www.hamilton dot com part number 55751-01) and a segment of HPLC tubing. HPLC tubing within my field of view can have an inside diameter as small as 0.005 inch. hope that helps, Happy Merry, etc., Dan On 12/15/2014 11:09 AM, Thomas Cleveland wrote: Hi all, I'm trying to put together some homemade syringe couplers following the published instructions from the Caffrey group. I'm having a bit of trouble with this part: The stainless steel ferrule of the second needle is removed and placed on the free end of the coupling needle such that the double thumb nut is held symmetrically between the two steel ferrules Has anyone done this, and if so, how did you remove the stainless steel ferrule from the second needle in order to place it over the first? The stainless steel ferrule appears to be firmly attached and I'm having trouble removing it. Thanks, Thomas Cleveland
Re: [ccp4bb] Fabricating hamilton syringe coupler for LCP preparation
Hi Thomas, I have been doing LCP for a while and I am using the teflon ferrules that come with the 250 uL RN syringes instead. So on one side you would have two teflon ferrules and the other side you would have the ferrule that is attached to the needle and another plastic ferrule. I have also had luck using a 100 uL teflon ferrule (you have to hollow it out a bit) for this purpose. We have a machine shop here that can do the ferrule removal. I didn't have much luck doing this on my own. Hope that helps, Jarrod Mousa Graduate Research Assistant University of Florida -Jarrod Mousa On Mon, Dec 15, 2014 at 2:09 PM, Thomas Cleveland thomas.clevel...@gmail.com wrote: Hi all, I'm trying to put together some homemade syringe couplers following the published instructions from the Caffrey group. I'm having a bit of trouble with this part: The stainless steel ferrule of the second needle is removed and placed on the free end of the coupling needle such that the double thumb nut is held symmetrically between the two steel ferrules Has anyone done this, and if so, how did you remove the stainless steel ferrule from the second needle in order to place it over the first? The stainless steel ferrule appears to be firmly attached and I'm having trouble removing it. Thanks, Thomas Cleveland
Re: [ccp4bb] Fabricating hamilton syringe coupler for LCP preparation
correction: www.hamiltoncompany.com addition: The compression fittings at least used to be available from us.vwr.com part number 89187-0002. On 12/15/2014 11:48 AM, Daniel Anderson wrote: Here's my addition to Jim Fairman's reply: You could use a pair of RN compression fittings (www.hamilton dot com part number 55751-01) and a segment of HPLC tubing. HPLC tubing within my field of view can have an inside diameter as small as 0.005 inch. hope that helps, Happy Merry, etc., Dan On 12/15/2014 11:09 AM, Thomas Cleveland wrote: Hi all, I'm trying to put together some homemade syringe couplers following the published instructions from the Caffrey group. I'm having a bit of trouble with this part: The stainless steel ferrule of the second needle is removed and placed on the free end of the coupling needle such that the double thumb nut is held symmetrically between the two steel ferrules Has anyone done this, and if so, how did you remove the stainless steel ferrule from the second needle in order to place it over the first? The stainless steel ferrule appears to be firmly attached and I'm having trouble removing it. Thanks, Thomas Cleveland
Re: [ccp4bb] Fabricating hamilton syringe coupler for LCP preparation
Tried the RN kludge at least I did not get it to work. You cannot tighten the plastic swage lock type sleeves tight enough. On operation the pressure drives the PEEK tubing out of the compression fit. Maybe if you have a jig that holds the 2 syringes in a fixed position so they cannot move apart it can work. Best, BR -Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Daniel Anderson Sent: Monday, December 15, 2014 8:48 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Fabricating hamilton syringe coupler for LCP preparation Here's my addition to Jim Fairman's reply: You could use a pair of RN compression fittings (www.hamilton dot com part number 55751-01) and a segment of HPLC tubing. HPLC tubing within my field of view can have an inside diameter as small as 0.005 inch. hope that helps, Happy Merry, etc., Dan On 12/15/2014 11:09 AM, Thomas Cleveland wrote: Hi all, I'm trying to put together some homemade syringe couplers following the published instructions from the Caffrey group. I'm having a bit of trouble with this part: The stainless steel ferrule of the second needle is removed and placed on the free end of the coupling needle such that the double thumb nut is held symmetrically between the two steel ferrules Has anyone done this, and if so, how did you remove the stainless steel ferrule from the second needle in order to place it over the first? The stainless steel ferrule appears to be firmly attached and I'm having trouble removing it. Thanks, Thomas Cleveland
Re: [ccp4bb] Fabricating hamilton syringe coupler for LCP preparation
I agree with Jim – purchasing the couplers will get you up and running much quicker. TTP also sells nice couplers: https://www.ttplabtechstore.com/ttp_ecom/cc/ItemDetails.jsp?@where.ItemID@EQ=3072-01050sessionkey=# Aaron On Mon, Dec 15, 2014 at 12:38 PM, Bernhard Rupp hofkristall...@gmail.com wrote: Tried the RN kludge at least I did not get it to work. You cannot tighten the plastic swage lock type sleeves tight enough. On operation the pressure drives the PEEK tubing out of the compression fit. Maybe if you have a jig that holds the 2 syringes in a fixed position so they cannot move apart it can work. Best, BR -Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Daniel Anderson Sent: Monday, December 15, 2014 8:48 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Fabricating hamilton syringe coupler for LCP preparation Here's my addition to Jim Fairman's reply: You could use a pair of RN compression fittings (www.hamilton dot com part number 55751-01) and a segment of HPLC tubing. HPLC tubing within my field of view can have an inside diameter as small as 0.005 inch. hope that helps, Happy Merry, etc., Dan On 12/15/2014 11:09 AM, Thomas Cleveland wrote: Hi all, I'm trying to put together some homemade syringe couplers following the published instructions from the Caffrey group. I'm having a bit of trouble with this part: The stainless steel ferrule of the second needle is removed and placed on the free end of the coupling needle such that the double thumb nut is held symmetrically between the two steel ferrules Has anyone done this, and if so, how did you remove the stainless steel ferrule from the second needle in order to place it over the first? The stainless steel ferrule appears to be firmly attached and I'm having trouble removing it. Thanks, Thomas Cleveland
Re: [ccp4bb] Fabricating hamilton syringe coupler for LCP preparation
Hi everyone, Thanks for the advice. I had, in fact, already ordered some commercial couplers, but they haven't come in yet, and there was an experiment I wanted to do today. Here's what I found: 1. The steel ferrules have an orientation, with a tight side and a loose side. They can be removed by sliding in one direction, but not the other. Even then, it's pretty difficult. I had to use pliers, and pieces of needle broke off during the process. The tight side of the ferrule then needed to be reamed open slightly with a steel tool before I was able to slide the ferrule onto the other needle. 2. Soldering stainless steel is really a pain. In the end I got a coupler that seems to work well, but it was a pain, and it's a bit charred looking. Thanks again, Tom On Mon, Dec 15, 2014 at 6:01 PM, Aaron Thompson aaron.a.thomp...@gmail.com wrote: I agree with Jim – purchasing the couplers will get you up and running much quicker. TTP also sells nice couplers: https://www.ttplabtechstore.com/ttp_ecom/cc/ItemDetails.jsp?@where.ItemID@EQ=3072-01050sessionkey=# Aaron On Mon, Dec 15, 2014 at 12:38 PM, Bernhard Rupp hofkristall...@gmail.com wrote: Tried the RN kludge at least I did not get it to work. You cannot tighten the plastic swage lock type sleeves tight enough. On operation the pressure drives the PEEK tubing out of the compression fit. Maybe if you have a jig that holds the 2 syringes in a fixed position so they cannot move apart it can work. Best, BR -Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Daniel Anderson Sent: Monday, December 15, 2014 8:48 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Fabricating hamilton syringe coupler for LCP preparation Here's my addition to Jim Fairman's reply: You could use a pair of RN compression fittings (www.hamilton dot com part number 55751-01) and a segment of HPLC tubing. HPLC tubing within my field of view can have an inside diameter as small as 0.005 inch. hope that helps, Happy Merry, etc., Dan On 12/15/2014 11:09 AM, Thomas Cleveland wrote: Hi all, I'm trying to put together some homemade syringe couplers following the published instructions from the Caffrey group. I'm having a bit of trouble with this part: The stainless steel ferrule of the second needle is removed and placed on the free end of the coupling needle such that the double thumb nut is held symmetrically between the two steel ferrules Has anyone done this, and if so, how did you remove the stainless steel ferrule from the second needle in order to place it over the first? The stainless steel ferrule appears to be firmly attached and I'm having trouble removing it. Thanks, Thomas Cleveland
Re: [ccp4bb] Fabricating hamilton syringe coupler for LCP preparation
Art Robbins Instruments also sells a zero-length coupler and it works well. BR From: Aaron Thompson [mailto:aaron.a.thomp...@gmail.com] Sent: Tuesday, December 16, 2014 12:02 AM To: b...@hofkristallamt.org Cc: CCP4BB@jiscmail.ac.uk Subject: Re: [ccp4bb] Fabricating hamilton syringe coupler for LCP preparation I agree with Jim – purchasing the couplers will get you up and running much quicker. TTP also sells nice couplers: https://www.ttplabtechstore.com/ttp_ecom/cc/ItemDetails.jsp?@where.ItemID@EQ=3072-01050sessionkey= https://www.ttplabtechstore.com/ttp_ecom/cc/ItemDetails.jsp?@where.ItemID@EQ=3072-01050sessionkey=# Aaron On Mon, Dec 15, 2014 at 12:38 PM, Bernhard Rupp hofkristall...@gmail.com wrote: Tried the RN kludge at least I did not get it to work. You cannot tighten the plastic swage lock type sleeves tight enough. On operation the pressure drives the PEEK tubing out of the compression fit. Maybe if you have a jig that holds the 2 syringes in a fixed position so they cannot move apart it can work. Best, BR -Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Daniel Anderson Sent: Monday, December 15, 2014 8:48 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Fabricating hamilton syringe coupler for LCP preparation Here's my addition to Jim Fairman's reply: You could use a pair of RN compression fittings (www.hamilton dot com part number 55751-01) and a segment of HPLC tubing. HPLC tubing within my field of view can have an inside diameter as small as 0.005 inch. hope that helps, Happy Merry, etc., Dan On 12/15/2014 11:09 AM, Thomas Cleveland wrote: Hi all, I'm trying to put together some homemade syringe couplers following the published instructions from the Caffrey group. I'm having a bit of trouble with this part: The stainless steel ferrule of the second needle is removed and placed on the free end of the coupling needle such that the double thumb nut is held symmetrically between the two steel ferrules Has anyone done this, and if so, how did you remove the stainless steel ferrule from the second needle in order to place it over the first? The stainless steel ferrule appears to be firmly attached and I'm having trouble removing it. Thanks, Thomas Cleveland
