I would try phase improvement, especially since you have
two molecules per a.u. In other words averaging, solvent
flattning, histogram matching. The best way is to start at
low(er) resoltuion and extend to the highest resolution
vailable. The best criterium for success is a improved and
interpretable map.
I had quite some success with this appraoch with MR
solutions, but uninterpretable or difficult to build maps.
Good luck,
Klaus
Dr. Klaus Piontek
Albert-Ludwigs University Freiburg
Institute of Organic Chemistry and Biochemistry, Room 401 H
Albertstrasse 21
D-79104 Freiburg
Germany
Phone: ++49-761-203-6036
Fax: ++49-761-203-8714
Email: klaus.pion...@ocbc.uni-freiburg.de
--- the forwarded message follows ---
---BeginMessage---
Hi all,
I have been attempting to find a MR solution for a low resolution data set
(3.9A), with pretty poor merging stats of a 22 strand membrane beta barrel I'm
working on.
I've created a trimmed poly-alanine from a structure of 17% identity, that
gives a solution with a Tfz of 14.3 with two molecules per asu (llg is around
900). I'm guessing this in a genuine solution, but the map is too poor to build
into.
Does anyone have any advice as to proceed from here? It may be just a case of
needing better resolution data to work with, but would this indicate that
Selenomet derivative crystals won't be needed for this structure?
Cheers,
Rhys---End Message---
