Re: [ccp4bb] IPTG induced protein expression

[Dima Klenchin]

I was wondering if anyone has used regular IPTG (not IPTG-dioxane free, 
special

grade) for protein expression.
Are there any problems using such regular samples (5mg dioxane per Kg of IPTG)
for protein expression?


I have. No problem whatsoever. I belive the whole dioxane-free thing is 
about dioxane being carcinogen and has nothing to do with minute amounts of 
it affecting E.coli in any way.


The amounts of dioxane in regular grade IPTG is nothing in comparison to 
the carcinogenicity of the heavy metals we use for phasing (or even 
cigarette smoking).


Dima

[ccp4bb] IPTG induced protein expression

[nagem]

Dear CCP4bb users,

I was wondering if anyone has used regular IPTG (not IPTG-dioxane free, special
grade) for protein expression.
Are there any problems using such regular samples (5mg dioxane per Kg of IPTG)
for protein expression?

It would be great to have an idea for real problems if using this.

Cheers

Ronaldo.