Hi Everyone,
I am seeing something strange with HKL 3000R with regard to the mosaicity histogram and was wondering if anyone has come across something like it before. From the HKL 2000 manual I understand that the mosaicity histogram should to go through 0 close to 0.5 partiality, to have higher values in the negative zone and to go down to close to 0 at +mosaicity/2. I am working on a Rigaku system (XtaLAB MM003 , AFC11 Partial-χ 4-axis goniometer , PILATUS 200K detector) and it came with HKL 3000R. I have looked at quite a few different crystals from Lysozyme to RNA and in every case the first zone in the histogram is the 0 zone and it is (somewhat) close to 0.5 partiality and the histogram goes down from there to close to 0 at + mosaicity/2. But the histogram has nothing in negative zones. I am trying to understand how that can be and if this indicates a problem of some kind. Or maybe I am simply doing something stupid wrong? I am using a 3D window that covers about twice the mosaicity. I have seen it with data that were collected with 0.25, 0.5 or 1 degree oscillation. I appreciate any advice. Thanks - Annette Annette H. Erbse Department of Chemistry & Biochemistry University of Colorado at Boulder Voice: ++1-303-492-0528 (office) Fax: ++1-303-492-8425 Email: er...@colorado.edu<mailto:er...@colorado.edu> Office: JSCB C316
