[ccp4bb] Most Abundant Eukaryotic Membrane Protein List?
Dear List, Does anyone know of a source of quantification of membrane proteins in garden-variety eukaryotic cell lines, e.g. HEK or HeLa cells (incidentally, it just occurred to me that probably all HeLa cells are XX--seems right, no?) I am looking for the highest-expressed, particularly, and definitely want to include single-pass proteins as well. Jacob -- *** Jacob Pearson Keller, PhD Looger Lab/HHMI Janelia Farms Research Campus 19700 Helix Dr, Ashburn, VA 20147 email: kell...@janelia.hhmi.org ***
Re: [ccp4bb] Most Abundant Eukaryotic Membrane Protein List?
In the mitocondrial inner membrane (which is very protein-dense) I believe the most abundant protein is the adenine nucleotide transporter. (e.g. pdb1OKC). Single chain or homodimer, but apparently its not very easy to crystallize. Jacob Keller wrote: Dear List, Does anyone know of a source of quantification of membrane proteins in garden-variety eukaryotic cell lines, e.g. HEK or HeLa cells (incidentally, it just occurred to me that probably all HeLa cells are XX--seems right, no?) I am looking for the highest-expressed, particularly, and definitely want to include single-pass proteins as well. Jacob -- *** Jacob Pearson Keller, PhD Looger Lab/HHMI Janelia Farms Research Campus 19700 Helix Dr, Ashburn, VA 20147 email: kell...@janelia.hhmi.org mailto:kell...@janelia.hhmi.org ***
Re: [ccp4bb] Most Abundant Eukaryotic Membrane Protein List?
I don't want to crystallize the protein--I have another reason Jacob On Mon, Mar 18, 2013 at 1:18 PM, Edward A. Berry ber...@upstate.edu wrote: In the mitocondrial inner membrane (which is very protein-dense) I believe the most abundant protein is the adenine nucleotide transporter. (e.g. pdb1OKC). Single chain or homodimer, but apparently its not very easy to crystallize. Jacob Keller wrote: Dear List, Does anyone know of a source of quantification of membrane proteins in garden-variety eukaryotic cell lines, e.g. HEK or HeLa cells (incidentally, it just occurred to me that probably all HeLa cells are XX--seems right, no?) I am looking for the highest-expressed, particularly, and definitely want to include single-pass proteins as well. Jacob -- * Jacob Pearson Keller, PhD Looger Lab/HHMI Janelia Farms Research Campus 19700 Helix Dr, Ashburn, VA 20147 email: kell...@janelia.hhmi.org mailto:kell...@janelia.hhmi.**orgkell...@janelia.hhmi.org * -- *** Jacob Pearson Keller, PhD Looger Lab/HHMI Janelia Farms Research Campus 19700 Helix Dr, Ashburn, VA 20147 email: kell...@janelia.hhmi.org ***
Re: [ccp4bb] Most Abundant Eukaryotic Membrane Protein List?
BLASPHEMY! haha From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Jacob Keller Sent: Monday, March 18, 2013 1:22 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Most Abundant Eukaryotic Membrane Protein List? I don't want to crystallize the protein--I have another reason Jacob On Mon, Mar 18, 2013 at 1:18 PM, Edward A. Berry ber...@upstate.edumailto:ber...@upstate.edu wrote: In the mitocondrial inner membrane (which is very protein-dense) I believe the most abundant protein is the adenine nucleotide transporter. (e.g. pdb1OKC). Single chain or homodimer, but apparently its not very easy to crystallize. Jacob Keller wrote: Dear List, Does anyone know of a source of quantification of membrane proteins in garden-variety eukaryotic cell lines, e.g. HEK or HeLa cells (incidentally, it just occurred to me that probably all HeLa cells are XX--seems right, no?) I am looking for the highest-expressed, particularly, and definitely want to include single-pass proteins as well. Jacob -- *** Jacob Pearson Keller, PhD Looger Lab/HHMI Janelia Farms Research Campus 19700 Helix Dr, Ashburn, VA 20147 email: kell...@janelia.hhmi.orgmailto:kell...@janelia.hhmi.org mailto:kell...@janelia.hhmi.orgmailto:kell...@janelia.hhmi.org *** -- *** Jacob Pearson Keller, PhD Looger Lab/HHMI Janelia Farms Research Campus 19700 Helix Dr, Ashburn, VA 20147 email: kell...@janelia.hhmi.orgmailto:kell...@janelia.hhmi.org *** Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system.
Re: [ccp4bb] Most Abundant Eukaryotic Membrane Protein List?
I wouldn't be so sure that HeLa is just XX, or that it should be called garden variety after what I read today. http://www.nature.com/news/most-popular-human-cell-in-science-gets-sequenced-1.12609#/b1 -- David On 18 March 2013 15:08, Jacob Keller j-kell...@fsm.northwestern.edu wrote: Dear List, Does anyone know of a source of quantification of membrane proteins in garden-variety eukaryotic cell lines, e.g. HEK or HeLa cells (incidentally, it just occurred to me that probably all HeLa cells are XX--seems right, no?) I am looking for the highest-expressed, particularly, and definitely want to include single-pass proteins as well. Jacob -- *** Jacob Pearson Keller, PhD Looger Lab/HHMI Janelia Farms Research Campus 19700 Helix Dr, Ashburn, VA 20147 email: kell...@janelia.hhmi.org ***
