[ccp4bb] Off-topic: heterologous co-expression in yeast

[Andre Luis Berteli Ambrosio]

Hi,
We have been thinking of implementing recombinant protein expression in S. 
cerevisiae in our lab, and I would like to ask if there is a choice of vector 
designed for co-expression of any two intracellular binding partners, similarly 
to the pETduet-1 vector for E. coli.
If you feel this is not of broad interest, please reply directly to me.
I thank you for your input,

 Andre LB Ambrosio
Laboratório Nacional de Biociências - LNBio
CNPEM, Brazil

Re: [ccp4bb] Off-topic: heterologous co-expression in yeast

[Pascal Egea]

Hi Andre,

There is set of plasmids allowing coexpression in yeast
they are described in the following article

Constructionand characterization of bidirectional expression

vectors in Saccharomyces cerevisiae

Aimin Li1,2, Zengshan Liu1, Qianxue Li2, Lu Yu1, Dacheng Wang2  Xuming Deng
1,2


in FEMS Yeast Res 8 (2008) 6–9 !c


Those plasmids are designed for coexpression and use two different
promoters a constitutive one (GPD) and a strong inducible one (GAL). You
have several selection markers and they are shuttle vectors for easy
manipulation and amplification in E. coli.


Hope this helps.


Best of luck



-- 
Pascal F. Egea, PhD
Assistant Professor
UCLA, David Geffen School of Medicine
Department of Biological Chemistry
Boyer Hall room 356
611 Charles E Young Drive East
Los Angeles CA 90095
office (310)-983-3515
lab  (310)-983-3516
email pe...@mednet.ucla.edu