Dear Wei,
doesnt sound too special to me. DNA in itself is a very soluble
molecule. The part which hangs out of
the core complex drives solubility of the whole thing.
As a drawback crystallizability may also go down...
All the best,
Matthias
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Dr. Matthias Zebisch
Division of Structural Biology,
Wellcome Trust Centre for Human Genetics,
University of Oxford,
Roosevelt Drive,
Oxford OX3 7BN, UK
Phone (+44) 1865 287549;
Fax (+44) 1865 287547
Email matth...@strubi.ox.ac.uk
Website http://www.strubi.ox.ac.uk
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On 10/2/2013 4:24 PM, Wei Huang wrote:
Dear CCP4er,
I have an interesting observation for one of my DNA binding protein--when the
length of DNA flanking both DNA binding site increase, the solubility of
protein-DNA complex increases. And I can get a nice sigmoid curve from protein
solubility versus DNA length. The solubility of protein complex saturated at
the DNA length with additional 12 base pairs of DNA on both sides.
Has anyone had similar observation before? What is the implication for this
DNA-binding protein? Thanks for sharing your opinions!
Best regards,
Wei
