Re: [ccp4bb] Poor baculovirus stability at 4C?
Hi, I agree with others here. We produce virus in homemade ISFM and supplement with 2% serum, then store at 4 C protected from light. We don't titer. I have noticed no difference in expression over 1-2 years. -Tom Cleveland On Sep 27, 2012 8:19 PM, Vitali Stanevich stanev...@wisc.edu wrote: Alexander, We produce baculovirus at Gibco SF900-II + 10% heated FBS + 1xPSG and then store at 4C routinely. And it seems to be quite stable. We have a virus that had no change for more than 2 years: we judge by the volume of it needed to infect_cells/produce_protein, no titer measurements. We also avoid exposure to light: definitely cover it with foil if it's transparent door fridge. Vitali On Thu, Sep 27, 2012 at 5:42 PM, aaleshin aales...@burnham.org wrote: Sorry for an off-topic...
[ccp4bb] Poor baculovirus stability at 4C?
Sorry for an off-topic question. We began experiencing a sudden reduction in stability of baculovirus stock stored at 4C, like the titer drops more than 5 fold in 3-5 month. The only difference compared with previous preparations is a switch from Gibco SF900-II to a media from Lonza (Insect-XPRESS). Could it be due to the media switch, or something else? What is the best way to store baculovirus? Thank you Alexander Aleshin Sanford-Burnham Medical Research Institute Infectious Inflammatory Disease Center 10901 North Torrey Pines Road La Jolla, California 92037
Re: [ccp4bb] Poor baculovirus stability at 4C?
We began experiencing a sudden reduction in stability of baculovirus stock stored at 4C, like the titer drops more than 5 fold in 3-5 month. The only difference compared with previous preparations is a switch from Gibco SF900-II to a media from Lonza (Insect-XPRESS). Could it be due to the media switch, or something else? What is the best way to store baculovirus? Absolutely could be. For long term storage, add 10% FBS. Helps A LOT. I've used 2 years old stocks stored in serum-containing medium and they worked fine. We don't titer (because it's kind of pointless) but the optimal virus to culture volume ratio has not changed more than twoi-fold. - Dima
Re: [ccp4bb] Poor baculovirus stability at 4C?
Alexander, We produce baculovirus at Gibco SF900-II + 10% heated FBS + 1xPSG and then store at 4C routinely. And it seems to be quite stable. We have a virus that had no change for more than 2 years: we judge by the volume of it needed to infect_cells/produce_protein, no titer measurements. We also avoid exposure to light: definitely cover it with foil if it's transparent door fridge. Vitali On Thu, Sep 27, 2012 at 5:42 PM, aaleshin aales...@burnham.org wrote: Sorry for an off-topic question. We began experiencing a sudden reduction in stability of baculovirus stock stored at 4C, like the titer drops more than 5 fold in 3-5 month. The only difference compared with previous preparations is a switch from Gibco SF900-II to a media from Lonza (Insect-XPRESS). Could it be due to the media switch, or something else? What is the best way to store baculovirus? Thank you Alexander Aleshin Sanford-Burnham Medical Research Institute Infectious Inflammatory Disease Center 10901 North Torrey Pines Road La Jolla, California 92037
