Re: [ccp4bb] Rhodamine in FPLC

[Artem Evdokimov]

30% acetic acid solution should wash it out (exercise some care, it's quite
a strong vinegar).

Hypochlorite and peroxide will bleach the stain, but technically speaking
the leftover oxidized species will still be stuck in there, just colorless.

Artem

www.harkerbio.com
"making lots of *really tiny* drops... for fun and profit"

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On Fri, Jun 9, 2017 at 6:21 PM, Tomas Malinauskas <
tomas.malinaus...@gmail.com> wrote:

> Dear Reza,
> I used hydrogen peroxide (~0.1-2%?) to remove rhodamine stuck on
> Superdex columns. "Pink" columns turned "white" after this kind of
> treatment.
> Hope that helps,
> Tomas
>
> On Fri, Jun 9, 2017 at 10:43 PM, Reza Khayat 
> wrote:
> > Hi,
> >
> > Sorry for the non crystallography question. We're using Rhodamine for
> labeling some material that we need to run through our FPLC. It seems that
> the Rhodamine is getting stuck to the PEEK tubing. Anyone has experience
> with successfully removing the Rhodamine from the tubing? Thanks.
> >
> > Best wishes,
> > Reza
> >
> > Reza Khayat, PhD
> > Assistant Professor
> > City College of New York
> > Department of Chemistry
> > New York, NY 10031
>

Re: [ccp4bb] Rhodamine in FPLC

[Tomas Malinauskas]

Dear Reza,
I used hydrogen peroxide (~0.1-2%?) to remove rhodamine stuck on
Superdex columns. "Pink" columns turned "white" after this kind of
treatment.
Hope that helps,
Tomas

On Fri, Jun 9, 2017 at 10:43 PM, Reza Khayat  wrote:
> Hi,
>
> Sorry for the non crystallography question. We're using Rhodamine for 
> labeling some material that we need to run through our FPLC. It seems that 
> the Rhodamine is getting stuck to the PEEK tubing. Anyone has experience with 
> successfully removing the Rhodamine from the tubing? Thanks.
>
> Best wishes,
> Reza
>
> Reza Khayat, PhD
> Assistant Professor
> City College of New York
> Department of Chemistry
> New York, NY 10031

[ccp4bb] Rhodamine in FPLC

[Reza Khayat]

Hi,

Sorry for the non crystallography question. We're using Rhodamine for labeling 
some material that we need to run through our FPLC. It seems that the Rhodamine 
is getting stuck to the PEEK tubing. Anyone has experience with successfully 
removing the Rhodamine from the tubing? Thanks.

Best wishes,
Reza

Reza Khayat, PhD
Assistant Professor
City College of New York
Department of Chemistry
New York, NY 10031