Re: [ccp4bb] Strange Ancient Diffraction Pattern...
-- Dr. Aaron Finke Postdoctoral Fellow Swiss Light Source WSLA/217 CH-5232 Villigen-PSI phone: +41 56 310 5652 e-mail: aaron.fi...@psi.ch On Apr 1, 2015, at 13:03, Keller, Jacob kell...@janelia.hhmi.org wrote: Can anyone index this? It's got mostly split spots and a strange diffuse scattering background JPK *** Jacob Pearson Keller, PhD Looger Lab/HHMI Janelia Research Campus 19700 Helix Dr, Ashburn, VA 20147 email: kell...@janelia.hhmi.org *** roundmatzah.jpg
Re: [ccp4bb] Strange Ancient Diffraction Pattern...
Ah Ha! drum roll A quasicrystal ! On Wed, Apr 1, 2015 at 7:00 AM, Julia Griese gri...@dbb.su.se wrote: This one appears to be of a similar age. It has a most puzzling, but pretty pentagonal pattern (and a backstop). Unfortunately Mosflm doesn't appear to support the image format. /Julia On 01/04/15 13:08, Harry Powell wrote: Hi Jacob I noticed that there's no backstop shadow that might give a clue as to the direct beam position. Do you know what wavelength radiation was used to bake this? On 1 Apr 2015, at 12:03, Keller, Jacob wrote: Can anyone index this? It's got mostly split spots and a strange diffuse scattering background JPK *** Jacob Pearson Keller, PhD Looger Lab/HHMI Janelia Research Campus 19700 Helix Dr, Ashburn, VA 20147 email: kell...@janelia.hhmi.org *** roundmatzah.jpg Harry -- Dr Harry Powell, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge CB2 0QH Chairman of International Union of Crystallography Commission on Crystallographic Computing Chairman of European Crystallographic Association SIG9 (Crystallographic Computing) -- Dr. Julia Griese Postdoctoral Researcher Stockholm Center for Biomembrane Research Department of Biochemistry and Biophysics Stockholm University 106 91 Stockholm Sweden phone: +46-(0)8-162 778 email: gri...@dbb.su.se
Re: [ccp4bb] Strange Ancient Diffraction Pattern...
Have you tried Shake-and-Bake? An old program, but works great when you have mixed real and reciprocal space cake. Keller, Jacob schreef: Can anyone index this? It's got mostly split spots and a strange diffuse scattering background JPK *** Jacob Pearson Keller, PhD Looger Lab/HHMI Janelia Research Campus 19700 Helix Dr, Ashburn, VA 20147 email: kell...@janelia.hhmi.org ***
Re: [ccp4bb] Strange Ancient Diffraction Pattern...
I think we used the same version of Mosflm when we managed to solve this strange pattern. [cid:4EF3709E-41D5-4A38-8CE9-064D75929422@uefad.uef.fi] Helena Taberman, M.Sc. University of Eastern Finland Department of Chemistry Joensuu Campus P.O. Box 111 FI-80101 Joensuu, FINLAND Mobile: +358 50 337 2488 Finke Aaron (PSI) aaron.fi...@psi.chmailto:aaron.fi...@psi.ch kirjoitti 1.4.2015 kello 15.27: I have seen this before, actually. It appears to be multiple lattices, but if you carefully index it with Loxflm (a less well-known version of Mosflm), I was able to get a solution from bagelecular replacement: Bagels-Sesame.jpg Note that the sesame seeds are disordered. Hope that helps, Aaron -- Dr. Aaron Finke Postdoctoral Fellow Swiss Light Source WSLA/217 CH-5232 Villigen-PSI phone: +41 56 310 5652 e-mail: aaron.fi...@psi.chmailto:aaron.fi...@psi.ch On Apr 1, 2015, at 13:03, Keller, Jacob kell...@janelia.hhmi.orgmailto:kell...@janelia.hhmi.org wrote: Can anyone index this? It's got mostly split spots and a strange diffuse scattering background JPK *** Jacob Pearson Keller, PhD Looger Lab/HHMI Janelia Research Campus 19700 Helix Dr, Ashburn, VA 20147 email: kell...@janelia.hhmi.orgmailto:kell...@janelia.hhmi.org *** roundmatzah.jpg
Re: [ccp4bb] Strange Ancient Diffraction Pattern...
no, and that’s why they all needed to be stored at Diamond in case future generations have better programs. And this applies also to those that have already been digested - I mean, processed. Adrian On 01 Apr 2015, at 12:03, Keller, Jacob kell...@janelia.hhmi.org wrote: Can anyone index this? It's got mostly split spots and a strange diffuse scattering background JPK *** Jacob Pearson Keller, PhD Looger Lab/HHMI Janelia Research Campus 19700 Helix Dr, Ashburn, VA 20147 email: kell...@janelia.hhmi.org *** roundmatzah.jpg signature.asc Description: Message signed with OpenPGP using GPGMail
Re: [ccp4bb] Strange Ancient Diffraction Pattern...
Hi Jacob I noticed that there's no backstop shadow that might give a clue as to the direct beam position. Do you know what wavelength radiation was used to bake this? On 1 Apr 2015, at 12:03, Keller, Jacob wrote: Can anyone index this? It's got mostly split spots and a strange diffuse scattering background JPK *** Jacob Pearson Keller, PhD Looger Lab/HHMI Janelia Research Campus 19700 Helix Dr, Ashburn, VA 20147 email: kell...@janelia.hhmi.org *** roundmatzah.jpg Harry -- Dr Harry Powell, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge CB2 0QH Chairman of International Union of Crystallography Commission on Crystallographic Computing Chairman of European Crystallographic Association SIG9 (Crystallographic Computing)
Re: [ccp4bb] Strange Ancient Diffraction Pattern...
This one appears to be of a similar age. It has a most puzzling, but pretty pentagonal pattern (and a backstop). Unfortunately Mosflm doesn't appear to support the image format. /Julia On 01/04/15 13:08, Harry Powell wrote: Hi Jacob I noticed that there's no backstop shadow that might give a clue as to the direct beam position. Do you know what wavelength radiation was used to bake this? On 1 Apr 2015, at 12:03, Keller, Jacob wrote: Can anyone index this? It's got mostly split spots and a strange diffuse scattering background JPK *** Jacob Pearson Keller, PhD Looger Lab/HHMI Janelia Research Campus 19700 Helix Dr, Ashburn, VA 20147 email: kell...@janelia.hhmi.org mailto:kell...@janelia.hhmi.org *** roundmatzah.jpg Harry -- Dr Harry Powell, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge CB2 0QH Chairman of International Union of Crystallography Commission on Crystallographic Computing Chairman of European Crystallographic Association SIG9 (Crystallographic Computing) -- Dr. Julia Griese Postdoctoral Researcher Stockholm Center for Biomembrane Research Department of Biochemistry and Biophysics Stockholm University 106 91 Stockholm Sweden phone: +46-(0)8-162 778 email: gri...@dbb.su.se
Re: [ccp4bb] Strange Ancient Diffraction Pattern...
Hi Julia, This was clearly taken at MAXlab, since it's a very distinct Swedish pattern. I would guess I911-2, unless of course it is a very old one from I711. There was a problem once, where the header of the file didn't get written correctly. Can you index it if you manually guess the beam position (somewhere behind the beamstop) and input it to imosflm? It should work, although you might have to try a number of guesses, seing the beamstop is so big. HTH, Folmer 2015-04-01 14:00 GMT+02:00 Julia Griese gri...@dbb.su.se: This one appears to be of a similar age. It has a most puzzling, but pretty pentagonal pattern (and a backstop). Unfortunately Mosflm doesn't appear to support the image format. /Julia On 01/04/15 13:08, Harry Powell wrote: Hi Jacob I noticed that there's no backstop shadow that might give a clue as to the direct beam position. Do you know what wavelength radiation was used to bake this? On 1 Apr 2015, at 12:03, Keller, Jacob wrote: Can anyone index this? It's got mostly split spots and a strange diffuse scattering background JPK *** Jacob Pearson Keller, PhD Looger Lab/HHMI Janelia Research Campus 19700 Helix Dr, Ashburn, VA 20147 email: kell...@janelia.hhmi.org *** roundmatzah.jpg Harry -- Dr Harry Powell, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge CB2 0QH Chairman of International Union of Crystallography Commission on Crystallographic Computing Chairman of European Crystallographic Association SIG9 (Crystallographic Computing) -- Dr. Julia Griese Postdoctoral Researcher Stockholm Center for Biomembrane Research Department of Biochemistry and Biophysics Stockholm University 106 91 Stockholm Sweden phone: +46-(0)8-162 778 email: gri...@dbb.su.se -- Folmer Fredslund
Re: [ccp4bb] Strange Ancient Diffraction Pattern...
This looks like a tough cookie. IMO you'd be crackers to persist with this crystal form. It's certainly not going to be a piece of cake. Dr David C Briggs PhD http://about.me/david_briggs On 1 Apr 2015 12:07, Keller, Jacob kell...@janelia.hhmi.org wrote: Can anyone index this? It's got mostly split spots and a strange diffuse scattering background JPK *** Jacob Pearson Keller, PhD Looger Lab/HHMI Janelia Research Campus 19700 Helix Dr, Ashburn, VA 20147 email: kell...@janelia.hhmi.org ***
Re: [ccp4bb] Strange Ancient Diffraction Pattern...
These last puns just take the biscuit! Derek On 1 Apr 2015, at 16:52, David Briggs drdavidcbri...@gmail.commailto:drdavidcbri...@gmail.com wrote: This looks like a tough cookie. IMO you'd be crackers to persist with this crystal form. It's certainly not going to be a piece of cake. Dr David C Briggs PhD http://about.me/david_briggs On 1 Apr 2015 12:07, Keller, Jacob kell...@janelia.hhmi.orgmailto:kell...@janelia.hhmi.org wrote: Can anyone index this? It's got mostly split spots and a strange diffuse scattering background JPK *** Jacob Pearson Keller, PhD Looger Lab/HHMI Janelia Research Campus 19700 Helix Dr, Ashburn, VA 20147 email: kell...@janelia.hhmi.orgmailto:kell...@janelia.hhmi.org ***
Re: [ccp4bb] Strange Ancient Diffraction Pattern...
These diffraction patterns, called matzo (Hebrew: מַצָּה) have been observed already by the ancient jews, and it is thought that the invention of the millstone (see http://galleryhip.com/millstone.html) is a consequence of early detector technology to produce these images. Andrea. On 01/04/2015 12:03, Keller, Jacob wrote: Can anyone index this? It's got mostly split spots and a strange diffuse scattering background JPK *** Jacob Pearson Keller, PhD Looger Lab/HHMI Janelia Research Campus 19700 Helix Dr, Ashburn, VA 20147 email: kell...@janelia.hhmi.org *** -- Dr Andrea Thorn MRC Laboratory of Molecular Biology Francis Crick Avenue Cambridge Biomedical Campus Cambridge CB2 0QH, U.K. andrea.th...@web.de http://shelx.uni-ac.gwdg.de/~athorn
Re: [ccp4bb] Strange Ancient Diffraction Pattern...
Gloria, I think this one might be modulated, you can clearly distinguish Bragg and satellite reflections. -j. On Wed, 2015-04-01 at 07:06 -0500, Gloria Borgstahl wrote: Ah Ha! drum roll A quasicrystal ! On Wed, Apr 1, 2015 at 7:00 AM, Julia Griese gri...@dbb.su.se wrote: This one appears to be of a similar age. It has a most puzzling, but pretty pentagonal pattern (and a backstop). Unfortunately Mosflm doesn't appear to support the image format. /Julia On 01/04/15 13:08, Harry Powell wrote: Hi Jacob I noticed that there's no backstop shadow that might give a clue as to the direct beam position. Do you know what wavelength radiation was used to bake this? On 1 Apr 2015, at 12:03, Keller, Jacob wrote: Can anyone index this? It's got mostly split spots and a strange diffuse scattering background JPK *** Jacob Pearson Keller, PhD Looger Lab/HHMI Janelia Research Campus 19700 Helix Dr, Ashburn, VA 20147 email: kell...@janelia.hhmi.org *** roundmatzah.jpg Harry -- Dr Harry Powell, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge CB2 0QH Chairman of International Union of Crystallography Commission on Crystallographic Computing Chairman of European Crystallographic Association SIG9 (Crystallographic Computing) -- Dr. Julia Griese Postdoctoral Researcher Stockholm Center for Biomembrane Research Department of Biochemistry and Biophysics Stockholm University 106 91 Stockholm Sweden phone: +46-(0)8-162 778 email: gri...@dbb.su.se
Re: [ccp4bb] Strange Ancient Diffraction Pattern...
Working at the MAX IV Laboratory (as it is called these days) I can indeed confirm it is a Swedish pattern, but I have not yet found a trace of the data itself, so I am not so sure it was collected here... However, as we will open the brightest synchrotron source in the world next year, we can offer beam time and assistance to solve this problem, maybe a good day to test this further would be 1-4-2017 best regards Marjolein On 01 Apr 2015, at 16:01, Folmer Fredslund folm...@gmail.commailto:folm...@gmail.com wrote: Hi Julia, This was clearly taken at MAXlab, since it's a very distinct Swedish pattern. I would guess I911-2, unless of course it is a very old one from I711. There was a problem once, where the header of the file didn't get written correctly. Can you index it if you manually guess the beam position (somewhere behind the beamstop) and input it to imosflm? It should work, although you might have to try a number of guesses, seing the beamstop is so big. HTH, Folmer 2015-04-01 14:00 GMT+02:00 Julia Griese gri...@dbb.su.semailto:gri...@dbb.su.se: This one appears to be of a similar age. It has a most puzzling, but pretty pentagonal pattern (and a backstop). Unfortunately Mosflm doesn't appear to support the image format. /Julia haijgghe..jpeg On 01/04/15 13:08, Harry Powell wrote: Hi Jacob I noticed that there's no backstop shadow that might give a clue as to the direct beam position. Do you know what wavelength radiation was used to bake this? On 1 Apr 2015, at 12:03, Keller, Jacob wrote: Can anyone index this? It's got mostly split spots and a strange diffuse scattering background JPK *** Jacob Pearson Keller, PhD Looger Lab/HHMI Janelia Research Campus 19700 Helix Dr, Ashburn, VA 20147 email: kell...@janelia.hhmi.orgmailto:kell...@janelia.hhmi.org *** roundmatzah.jpg Harry -- Dr Harry Powell, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge CB2 0QH Chairman of International Union of Crystallography Commission on Crystallographic Computing Chairman of European Crystallographic Association SIG9 (Crystallographic Computing) -- Dr. Julia Griese Postdoctoral Researcher Stockholm Center for Biomembrane Research Department of Biochemistry and Biophysics Stockholm University 106 91 Stockholm Sweden phone: +46-(0)8-162 778 email: gri...@dbb.su.semailto:gri...@dbb.su.se -- Folmer Fredslund [cid:3A2194AC-734C-45EB-B5A7-CE7FC0F0F1BC] Dr. Marjolein Thunnissen Science Coordinator Structural Biology MAX IV Laboratory Lund University P.O. Box 118, SE-221 00 Lund, Sweden Visiting address: Ole Römers väg 1, 223 63 Lund Telephone: +46 766 32 04 17 www.maxlab.lu.sehttp://www.maxlab.lu.se/
